Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

HAVCR1

Uniprot No.

Q96D42

Research Area

Immunology

Alternative Names

CD365; HAVCR 1 ; HAVCR; HAVcr-1; Havcr1; Hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM 1; KIM-1; T cell immunoglobin domain and mucin domain protein 1; T cell immunoglobulin mucin family member 1; T cell immunoglobulin mucin receptor 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell membrane protein 1; TIM; TIM-1; TIM1; TIMD 1; TIMD-1; TIMD1; TIMD1_HUMAN

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

21-290aa

Target Protein Sequence

SVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLLGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKITVSLEIVPPKVTTTPIVTTVPTVTTVRTSTTVPTTTTVPMTTVPTTTVPTTMSIPTTTTVLTTMTVSTTTSVPTTTSIPTTTSVPVTTTVSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAIRREPTSSPLYSYTTDGNDTVTESSDGLWNNNQTQLFLEHSLLTA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

59.1

Protein Length

Partial

Tag Info

C-terminal hFc1-Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To express the recombinant Human HAVCR1 protein in mammalian cells, a DNA fragment encoding the Human HAVCR1 protein (21-290aa) is inserted into a plasmid vector and transferred to the mammalian cells. Cells containing the plasmid are screened, cultured, and induced to express the HAVCR1 protein. The protein carries a C-terminal hFc-Myc tag. Lysing the cells allows for the collection of the recombinant Human HAVCR1 protein, which is purified through affinity purification and then identified through SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the recombinant Human HAVCR1 protein obtained is greater than 85%.

Target Background

Function

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.; (Microbial infection) Acts as a receptor for Hepatitis A virus.; (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane. Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP.; (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane.; (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E.

Gene References into Functions

neither the inflammatory biomarker calprotectin, nor the tubular biomarkers NGAL and KIM-1, provide robust prognostic information on the loss of renal function in a heterogeneous CKD population. All of them, however, are candidate prognostic biomarkers in primarily inflammatory renal diseases. PMID: 30078006
High KIM1 expression is associated with Clear Cell Renal Cell Carcinoma. PMID: 29295730
Human oligodendrocytes undergo apoptosis when exposed to Sema4A and take up H-ferritin for meeting iron requirements and that these functions are mediated via the Tim-1 receptor. H-ferritin can block Sema4A-mediated cytotoxicity. Sema4A is detectable in the CSF of multiple sclerosis patients and HIV-seropositive persons and can induce oligodendrocyte cell death. PMID: 29457657
Elevated plasma NT-proBNP and KIM-1 in patients with stage 5 chronic kidney disease (CKD5) undergoing maintenance hemodialysis (HD) and HF (+) (heart failure)CKD5-HD patients suggest that natriuretic peptides and KIM-1 may contribute to the pathogenesis of HF in CKD5-HD patients. PMID: 28990414
These findings suggest that the TIMD4-HAVCR1 variants may be the genetic risk factors for coronary heart disease and ischemic strok PMID: 29208769
These studies provide evidence that, in the absence of a functional alpha-dystroglycan, TIM-1 mediates the entry of Lassa virus pseudoviral particles through interactions of virions with the IgV PtdSer-binding pocket of TIM-1. PMID: 29875238
total flavone of Desmodium styracifolium inhibited HK-2 cell apoptosis and autophagy by regulating KIM-1 via p38/MAPK pathway. PMID: 29071538
Polymorphism +4259A>C in exon 3 of the TIM-3 gene is associated with susceptibility to multiple sclerosis but polymorphism -1637C>T in the promoter region of TIM-1 is not. PMID: 29141799
High KIM1 expression is associated with decreased proximal tubular epithelial cell function. PMID: 29045706
findings show that TIM1 is not an essential cellular receptor for hepatitis A virus entry into cultured cells PMID: 28874468
Urinary excretion of KIM-1 correlates with blood lead levels better than other traditional renal injury biomarkers, including N-acetyl-beta-(D)-glucosaminidase, alpha1-microglobulin, and beta2-microglobulin. PMID: 27966578
promising results were obtained for circulating KIM-1 prediction of renal outcomes in type 1 diabetes--{REVIEW} PMID: 27771693
data show that in addition to the phospholipid-binding function of HAVCR1, the enhancement of HCV infection involves other determinants in the IgV domain of HAVCR1. These findings expand the repertoire of molecules that HCV uses for cell entry, adding to the already complex mechanism of HCV infection and pathogenesis. PMID: 29321304
Review/Meta-analysis: suggest that TIM-1-416G[C single nucleotide polymorphism is associated with the risk of asthma for the Asian ethnicity in the codominant (G/G vs. G/C) and dominant (G/G vs. G/C + C/C) genetic models. PMID: 28434117
Urinary NGAL is a good predictor of mortality in established pediatric acute kidney injury (AKI) of heterogeneous etiology and outperforms calprotectin and KIM-1. Urinary calprotectin and KIM-1 predict renal replacement therapy requirement in pediatric AKI. PMID: 28409285
A panel consisting of IGFBP1, KIM1, GCLC and GSTM1 genes could be used in combination for early screening of CKDu, whereas these genes in addition with FN1, IGFBP3 and KLK1 could be used to monitor progression of CKDu. The regulation of these genes has to be studied on larger populations to validate their efficiency for further clinical use. PMID: 28103909
Quantification of TIM-3 and KIM-1 mRNA expressions, along with KIM-1 protein measurements in urine and blood could be employed as promising tools for noninvasive diagnosis of allograft dysfunction. PMID: 28757398
Report detection of drug-induced acute kidney injury in humans by combining the sensitivity of urinary KIM-1 along with urinary miR-21, -200c, and -423. PMID: 27122240
measurement of urinary and renal KIM-1 level may be helpful to evaluate severity of renal pathological damage and prognosis in adult Henoch-Schonlein purpura patients with nephritis PMID: 29122357
Urine KIM-1 level in acute kidney injury patients was significantly higher than that in the healthy controls. PMID: 28212617
PCNSL is characterized by frequent Tim-1 expression, and its soluble form in CSF may become a useful biomarker for PCNSL. PMID: 27709813
TIM-1 is a unique marker for the identification of a human IL-10(+) Breg subpopulation which is partially superimposed with transitional B cells PMID: 28103916
in the current meta-analysis, based on ten prospective studies involving 29366 participants, we evaluated the role of urinary tubular injury markers (NGAL, KIM-1 and NAG) in predicting clinical outcomes including CKD stage 3, end stage renal disease and mortality. PMID: 27907168
Urinary KIM-1 levels in the acute kidney injury (AKI) preterm infant group were not significantly higher than in no-AKI group on day of life 1, 3 and 7. PMID: 27020372
We conclude that KIM-1 might serve as a sensitive biomarker to screen children for kidney damage induced by environmental toxic agents, such as chromium and arsenic PMID: 27431456
These results suggest that a 6-amino acid deletion polymorphism in the mucin domain of TIM-1 protects from HIV-1 infection with a recessive effect. PMID: 27652980
The present study shows that serum and urine levels of NGAL and KIM-1 are higher in patients with acute kidney injury than in those without acute kidney injury, and that serum NGAL and the presence of chronic kidney disease are significant predictors of acute kidney injury in scrub typhus. PMID: 28419138
KIM-1 increases albumin endocytosis in renal tubule epithelial cells. PMID: 26332568
preliminary data indicates that KIM-1 expression may be associated with stage in Wilms Tumor PMID: 27562389
Urinary KIM-1 and NAG levels were positively correlated. PMID: 27272162
TLR2, Tim1, Tim3, TNF-alpha, IgE and EOS play a role in Mycoplasma pneumoniae Pneumonia-related wheezing in children. PMID: 27185658
The results of our study showed that there is no significant association between TIM-1 5383-5397ins/del and Multiple sclerosis (MS) (P = 0.38); however, the frequency of CT genotype of TIM-3 -1541C>T in patient group was significantly higher than the control group, and there was a significant association between CT genotype and MS PMID: 27091308
Study emphasizes a strong association of the tubular biomarker KIM-1 with chronic kidney disease progression in chronic heart failure and suggests usefulness as cardiorenal marker. PMID: 26422793
KIM-1 expression also enhances ERK MAPK activation, and the modulatory effect of KIM-1 on cellular repair process is likely mediated via ERK MAPK signaling pathway. PMID: 27084535
KIM-1 may mediate high glucose-induced autophagy and apoptosis in renal tubular epithelial cells. PMID: 27310004
Study suggests that TIM1 -416G>C and -1454G>A single nucleotide polymorphisms are associated with dilated cardiomyopathy susceptibility and prognosis in this Han Chinese population. PMID: 27818477
elevated baseline plasma KIM-1 strongly associated with risk of early progressive renal decline in type 1 diabetes PMID: 26509588
Urinary KIM-1 levels increased significantly in patients with contrast-induced nephropathy. PMID: 26474236
Data show that kidney injury molecule (KIM)-1 and neutrophil gelatinase-associated lipocalin (NGAL), were significantly elevated in welding workers post-exposure. PMID: 26673824
High NGAL and KIM-1 levels may indicate early diabetic kidney injury; however, we did not observe any relationship between these markers and diabetic indices PMID: 26393328
Kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin excretion rates correlated with excretion rates of other tubular damage markers and predicted outcome in patients with idiopathic membranous nephropathy PMID: 25762211
The aim of this review article is to summarize the literature data concerning KIM-1 as a potential novel marker in the early diagnosis and prediction of clinical outcome of certain cardiovascular diseases. PMID: 26697493
Activation of TIM1 by exposing the cells to TIM4 significantly increased the frequency of apoptotic colon cancer cells. The expression of FasL was increased in the cancer cells after treating by TIM4. PMID: 26921445
Urine KIM1 level was higher in kidney stone patients before lithotripsy than in healthy controls. PMID: 26407192
Urinary levels of NGAL are more sensitive than uKIM-1 and uL-FABP levels in predicting renal scarring in vesicoureteral reflux. PMID: 26324091
TIM-1 ligands increase the amount of cell surface protein, preventing its traffic to the immune synapse PMID: 26332704
Urinary KIM-1 measured 24 h after the start of drug infusion has the potential to detect early AKI in pediatric patients treated with MTX or platinum-class drugs PMID: 26248472
Urinary KIM-1 is a significant predictor of death in extremely low-birth-weight neonates. PMID: 26107391
Urinary KIM1 is significantly higher in patients with clear cell renal carcinoma than in controls. Urinary KIM1 decreases after nephrectomy in clear cell renal carcinoma patients. PMID: 27295912
decreased serum TIM-1 and TIM-3 levels and association of TIM-3 with nephritis suggest their possible role in the development and pathogenesis of systemic lupus erythematosus. PMID: 27049082

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily, TIM family

Tissue Specificity

Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.

Database Links

HGNC: 17866

OMIM: 606518

KEGG: hsa:26762

STRING: 9606.ENSP00000344844

UniGene: Hs.129711

Code	CSB-MP010144HU
Abbreviation	Recombinant Human HAVCR1 protein, partial
MSDS	MSDS Datasheet
Size	US$500
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Hepatitis A virus cellular receptor 1 (HAVCR1), partial

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