Code | CSB-EP010242HU1 |
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The preparation of this recombinant Human HDAC6 protein was to use gene recombination DNA technology to obtain a recombinant vector connected with a HDAC6 fragment (489-840aa) that could be translated into the HDAC6 protein and then transferred it into E.coli cells to express the recombinant HDAC6 protein molecule. In order to get the target protein with high purity, N-terminal 6xHis tag was used in the production. The purity is 90% determined by SDS-PAGE.
HDAC6, a member of class IIb, localizes largely in the cytoplasm and is the only HDAC isoform with two tandem catalytic domains. In addition, HDAC6 has a hydrolase-like zinc finger domain, that binds and transports polyubiquitinated protein aggregates, and a domain that regulates through protein–protein interactions the cytoskeleton protein tau, IIp45, and EGFR. Histone deacetylases (HDACs) are often involved in transcriptional regulation, cell cycle progression, and developmental events. Targets of its deacetylase activity include tubulin, cortactin, HSP (heat shock protein), and Bruchpilot proteins. In various models of neurodegenerative diseases, pharmacological inhibition of HDAC6 restores alpha-tubulin acetylation and mitochondrial transport. In addition, HDAC6 inhibitors (HDAC6i) facilitate the degradation of protein aggregates and/or protection from neuronal oxidative stress. Therefore, it plays a central role in the epigenetic regulation of CNS function, with emphasis on development, neurodegenerative diseases, and various mental disorders.
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