Recombinant Human Homeobox protein Hox-D10 (HOXD10)

Code CSB-YP010682HU
MSDS
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Source Yeast
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Code CSB-EP010682HU
MSDS
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Source E.coli
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Code CSB-EP010682HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010682HU
MSDS
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Source Baculovirus
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Code CSB-MP010682HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HOXD10
Uniprot No.
Alternative Names
AI385591; AI874987; Homeo box 4D; Homeo box D10 ; Homeobox D10; Homeobox protein Hox-4D; Homeobox protein Hox-4E; Homeobox protein Hox-D10; Hox 4.4; Hox 4.5; Hox 4D ; Hox 4E ; Hox 5.3; HOX4D ; HOX4E; HOXD10; HXD10; HXD10_HUMAN; OTTMUSP00000017845; RP23-313J15.9
Species
Homo sapiens (Human)
Expression Region
1-340
Target Protein Sequence
MSFPNSSPAA NTFLVDSLIS ACRSDSFYSS SASMYMPPPS ADMGTYGMQT CGLLPSLAKR EVNHQNMGMN VHPYIPQVDS WTDPNRSCRI EQPVTQQVPT CSFTTNIKEE SNCCMYSDKR NKLISAEVPS YQRLVPESCP VENPEVPVPG YFRLSQTYAT GKTQEYNNSP EGSSTVMLQL NPRGAAKPQL SAAQLQMEKK MNEPVSGQEP TKVSQVESPE AKGGLPEERS CLAEVSVSSP EVQEKESKEE IKSDTPTSNW LTAKSGRKKR CPYTKHQTLE LEKEFLFNMY LTRERRLEIS KSVNLTDRQV KIWFQNRRMK LKKMSRENRI RELTANLTFS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Gene References into Functions
  1. Results show that HOXD10 promoter is hypermethylated in papillary thyroid cancer (PTC) tissues and its mRNA expression decreased. Moreover, the hypermethylation of HOXD10 was associated with invasion of the primary tumor. Further data provide evidence that the epigenetic suppression of the HOXD10 gene may play a role in the tumorigenesis of PTC. PMID: 29115628
  2. Results show that HOXD10 is frequently methylated in human hepatocellular carcinoma (HCC), and the expression of HOXD10 is regulated by promoter region methylation. HOXD10 suppresses HCC cell growth both in vitro and in vivo. HOXD10 suppresses human HCC by inhibiting ERK signaling. PMID: 29075359
  3. our data suggest that the loss of HoxD10 function is common and may thus result in a progressive phenotype in PCa. HoxD10 may function as a biomarker that differentiates patients with BCR disease from the ones that are not after radical prostatectomy, implicating its potential as a therapeutic target. PMID: 28231752
  4. Knock down of the dickkopf WNT signaling pathway inhibitor 2 (DKK2) resulted in a significant suppression of HOXD10, HOXD11 and HOXD13 while over-expression of DKK2 and stimulation with factors of the WNT signaling pathway. PMID: 27363011
  5. By treating LECs with VEGF-C156S and analyzing subsequent changes in gene expression, we identified several 'immediate early' transcription factors that showed a rapid transient upregulation VEGFR-3 stimulation. these results reveal an important and unanticipated role of HOXD10 in the regulation of VEGFR-3 signaling in lymphatic endothelial cells, and in the control of lymphangiogenesis and permeability. PMID: 27199372
  6. Knockdown of HOXD10 in renal cells also resulted in increased resistance to colistin cytotoxicity. PMID: 28335481
  7. High expression of HOXD10 is associated with laryngeal squamous cell carcinoma. PMID: 27658780
  8. Low HOXD10 promotes migration and invasion in gastric cancer. PMID: 26311318
  9. These results suggested that HOXD10 may be a putative suppressor gene and can act as a prognostic marker and potentially a novel therapeutic target for cholangiocellular carcinoma. PMID: 26260613
  10. miR-23a was upregulated in glioma. This overexpression promoted glioma cell invasion, probably by modulating MMP-14 via directly inhibiting the expression of HOXD10. PMID: 24305689
  11. POU2F1 activity regulates HOXD10 and HOXD11 gene expression in head and neck squamous cell carcinoma, promoting a proliferative and invasive phenotype. PMID: 25301728
  12. Downregulation of the HOXD10 gene expression was associated with breast cancer. PMID: 25081374
  13. KLF4 and HOXD10 were identified as direct targets of miR-10b in bladder cancer cells PMID: 24573354
  14. It is a member of the HOX gene family. HOX genes are the main regulatory genes that directly influence organogenesis and maintain the function of differentiated tissues PMID: 24219032
  15. MicroRNA-10b promotes nucleus pulposus cell proliferation through RhoC-Akt pathway by targeting HOXD10 in intervetebral disc degeneration. PMID: 24376640
  16. HOXD10 expression varies by stage of disease and produces differential effects in head and neck squamous cell carcinoma. PMID: 25010866
  17. TBX1 can alter TGF-beta/BMP, an important signaling pathway, through interacting with HOXD10. Above findings may shed light on the mechanism of TBX1 mutations leading to renal malformations found in patients carrying a 22q11 deletion. PMID: 22842189
  18. downregulation of HOXD10 expression by miR-10b overexpression may induce an increase of pro-metastatic gene products, such as MMP14 and RHOC, and contribute to the acquisition of metastatic phenotypes in epithelial ovarian cancer cells PMID: 23670532
  19. HoxD10 potentially functions as a tumor suppressor that is inactivated through promoter hypermethylation in gastric cancer. PMID: 22160393
  20. Suggest that miR-10b can stimulate the upregulation of RhoC and AKT phosphorylation through targeting HOXD10, thus promoting cell invasion in gastric tumors. PMID: 22293682
  21. miR-10b induced glioma cell invasion by modulating tumor invasion factors MMP-14 and uPAR expression via the direct target HOXD10 PMID: 21419107
  22. results indicate a role for HoxD10 in maintaining a nonangiogenic state in the endothelium PMID: 12466126
  23. Missense Mutation in HOXD10 is associated with congenital vertical talus and Charcot-Marie-Tooth disease PMID: 15146389
  24. An autosomal-dominant-inherited mutation in a HOXD10 gene with complete penetrance is found in all members of a pedigree with congenital vertical talus. PMID: 15368082
  25. No evidence was found of linkage near the HOXD gene cluster on chromosome 2q, suggesting genes other than HOXD10 are responsible for idiopathic clubfoot. PMID: 17417092
  26. Pak1 is a target of miR-7 and that HoxD10 plays a regulatory role in modifying the expression of miR-7. PMID: 18922890

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Involvement in disease
Vertical talus, congenital (CVT)
Subcellular Location
Nucleus.
Protein Families
Abd-B homeobox family
Tissue Specificity
Strongly expressed in the adult male and female urogenital tracts.
Database Links

HGNC: 5133

OMIM: 142984

KEGG: hsa:3236

STRING: 9606.ENSP00000249501

UniGene: Hs.123070

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