Recombinant Human Inhibitor of growth protein 5 (ING5)

Code CSB-YP820185HU
MSDS
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Source Yeast
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Code CSB-EP820185HU
MSDS
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Source E.coli
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Code CSB-EP820185HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP820185HU
MSDS
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Source Baculovirus
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Code CSB-MP820185HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ING5
Uniprot No.
Alternative Names
1700001C14Rik; 1700027H23Rik; 1810018M11Rik; FLJ23842; ING 5; Ing5; ING5_HUMAN; Inhibitor of growth family member 5; Inhibitor of growth protein 5; p28 ING 5; p28 ING5; p28ING5
Species
Homo sapiens (Human)
Expression Region
1-240
Target Protein Sequence
MATAMYLEHY LDSIENLPCE LQRNFQLMRE LDQRTEDKKA EIDILAAEYI STVKTLSPDQ RVERLQKIQN AYSKCKEYSD DKVQLAMQTY EMVDKHIRRL DADLARFEAD LKDKMEGSDF ESSGGRGLKK GRGQKEKRGS RGRGRRTSEE DTPKKKKHKG GSEFTDTILS VHPSDVLDMP VDPNEPTYCL CHQVSYGEMI GCDNPDCPIE WFHFACVDLT TKPKGKWFCP RCVQEKRKKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the HBO1 complex, which specifically mediates acetylation of histone H3 at 'Lys-14' (H3K14ac) and, to a lower extent, acetylation of histone H4. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. Inhibits cell growth, induces a delay in S-phase progression and enhances Fas-induced apoptosis in an INCA1-dependent manner.
Gene References into Functions
  1. our results demonstrate, for the first time that overexpression of ING5 in osteosarcoma cells induces apoptosis PMID: 29528777
  2. ING5 promotes brain tumor initiating cells stemness by maintaining intracellular calcium levels and activating the follicle stimulating hormone pathway. PMID: 28925404
  3. Nucleocytoplasmic translocation of ING5 protein occurs in breast cancer, and the high expression of nuclear ING5 is inversely related to some poor clinicopathological behaviors of breast cancer. PMID: 29595458
  4. Overexpression of ING5 inhibited cell proliferation, neoplasm invasion and epithelial-mesenchymal transition of thyroid cancer cells. PMID: 29272787
  5. We revealed that miR-24 had the opposite effects to those of ING5 on breast cancer cells and could accelerate xenografted tumor growth in vivo. Our findings uncover the tumor-suppressive role of ING5 and the regulatory pathway of ING5 in breast cancer and may provide insights into the molecular mechanisms of breast carcinogenesis PMID: 28490335
  6. ING5 inhibits cell proliferation and invasion in esophageal squamous cell carcinoma through regulation of the Akt/NF-kappaB/MMP-9 signaling pathway. PMID: 29326045
  7. Data suggest that inhibitor of growth protein 5 (ING5) downregulation might involved in carcinogenesis, growth, and invasion of lung cancer and could be considered as a promising marker to gauge the aggressiveness of lung cancer. PMID: 27409347
  8. miR-1307 could promote ovarian cancer chemoresistance by targeting the ING5 expression and miR-1307 might serve as a therapeutic target for ovarian cancer. PMID: 28086946
  9. miR-331-3p is upregulated by HBV and promotes proliferation of hepatocellular carcinoma cells though repression of ING5 expression. PMID: 26497554
  10. Suggest that ING5 expression might be a good marker for gastric carcinogenesis and its subsequent progression by inhibiting proliferation, growth, migration, invasion and metastasis. PMID: 25980581
  11. Loss of ING5 expression is associated with lung cancer. PMID: 25938545
  12. Identified ING5 as a novel CDK2 substrate. ING5 is phosphorylated at a single site, threonine 152, by cyclin E/CDK2 and cyclin A/CDK2. This site is also phosphorylated in cells in a cell cycle dependent manner, consistent with it being a CDK2 substrate. PMID: 25860957
  13. Results showed that ING5 gene expression is inhibited by miR-193a-3p and is instrumental in miR-193a-3p's role in activating BCa chemoresistance. PMID: 25991669
  14. Data indicate that ING5 associates with Tip60 (KAT5) to form a complex with p53. PMID: 23576563
  15. ING5 down-regulation promotes bone mesenchymal stem cell proliferation. PMID: 22384930
  16. Results indicate that ING5 is a growth suppressor with suppressed expression in AML whose functions depend on its interaction with INCA1. PMID: 21750715
  17. EBNA3C negatively regulate p53-mediated functions by interacting with ING4 and ING5. PMID: 21177815
  18. Aberrant inhibitor of growth 5 expression may contribute to pathogenesis, growth, and invasion of colorectal carcinomas. PMID: 21193223
  19. ). Survival analysis indicated that nuclear ING5 was closely linked to favorable prognosis of carcinoma patients (P < .05), albeit not independent. PMID: 21062663
  20. Our study indicates for the first time, that oncogenic ras and loss of Smad signaling cooperate to upregulate EGFR and erbB2, which plays a role in promoting invasion. PMID: 20131318
  21. ING5 may function as a tumor suppressor gene or oncogene tightly linked with p53 status, and may play an important role in the prognosis of head and neck squamous cell carcinoma (HNSCC) patients. PMID: 20182888
  22. Tumor-specific mutation and down-regulation of ING5 mRNA suggested it as a tumor suppressor gene in oral squamous cell carcinoma PMID: 20131318
  23. p29ING4 and p28ING5 may be significant modulators of p53 function. PMID: 12750254
  24. the PHD domain of ING5 has the characteristic tertiary fold of a zinc finger. PMID: 18623064

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Subcellular Location
Nucleus. Chromosome.
Protein Families
ING family
Tissue Specificity
Down-regulated in bone marrow cells in acute myeloid leukemia patients as compared with normal bone marrow cells.
Database Links

HGNC: 19421

OMIM: 608525

KEGG: hsa:84289

STRING: 9606.ENSP00000322142

UniGene: Hs.529172

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