Recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit epsilon(IKBKE)

Code CSB-YP619863HU
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Source Yeast
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Code CSB-EP619863HU
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Source E.coli
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Code CSB-EP619863HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619863HU
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Source Baculovirus
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Code CSB-MP619863HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names IKBKE
Uniprot No. Q14164
Alternative Names I kappa B kinase epsilon; I-kappa-B kinase epsilon; IkBKE; IKK related kinase epsilon; IKK-E; IKK-epsilon; IKK-i; IKKE; IKKE_HUMAN; IKKepsilon; IKKI; Inducible I kappa B kinase; Inducible I kappa-B kinase; Inducible IkappaB kinase; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; KIAA0151; MGC125294; MGC125295; MGC125297
Species Homo sapiens (Human)
Expression Region 1-716
Target Protein Sequence MQSTANYLWH TDDLLGQGAT ASVYKARNKK SGELVAVKVF NTTSYLRPRE VQVREFEVLR KLNHQNIVKL FAVEETGGSR QKVLVMEYCS SGSLLSVLES PENAFGLPED EFLVVLRCVV AGMNHLRENG IVHRDIKPGN IMRLVGEEGQ SIYKLTDFGA ARELDDDEKF VSVYGTEEYL HPDMYERAVL RKPQQKAFGV TVDLWSIGVT LYHAATGSLP FIPFGGPRRN KEIMYRITTE KPAGAIAGAQ RRENGPLEWS YTLPITCQLS LGLQSQLVPI LANILEVEQA KCWGFDQFFA ETSDILQRVV VHVFSLSQAV LHHIYIHAHN TIAIFQEAVH KQTSVAPRHQ EYLFEGHLCV LEPSVSAQHI AHTTASSPLT LFSTAIPKGL AFRDPALDVP KFVPKVDLQA DYNTAKGVLG AGYQALRLAR ALLDGQELMF RGLHWVMEVL QATCRRTLEV ARTSLLYLSS SLGTERFSSV AGTPEIQELK AAAELRSRLR TLAEVLSRCS QNITETQESL SSLNRELVKS RDQVHEDRSI QQIQCCLDKM NFIYKQFKKS RMRPGLGYNE EQIHKLDKVN FSHLAKRLLQ VFQEECVQKY QASLVTHGKR MRVVHETRNH LRLVGCSVAA CNTEAQGVQE SLSKLLEELS HQLLQDRAKG AQASPPPIAP YPSPTRKDLL LHMQELCEGM KLLASDLLDN NRIIERLNRV PAPPDV
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.
Gene References into Functions
  1. fascin1 constitutively interacts with IkappaB kinase (IKK) in the RIG-I signaling pathway. In summary, we have identified fascin1 as a suppressor of the RIG-I signaling pathway associating with IkappaB kinase in DLD-1 colon cancer cells to suppress immune responses to viral infection. PMID: 29496994
  2. Single-Nucleotide Polymorphism in IKBKE gene had a strong association with resistance or susceptibility to dengue disease. PMID: 30332343
  3. These findings indicated that microRNA-98 could promote apoptosis of glioma cells via inhibiting inhibitor of kappa B kinase epsilon/nuclear factor-kappa B signaling and presented a novel regulatory pathway of microRNA-98 by direct suppression of inhibitor of kappa B kinase epsilon/nuclear factor-kappa B expression in glioma cells. PMID: 29333957
  4. In conclusion, the spindle cell morphology should be induced by RelA activation (p-RelA S468) by IKKepsilon upregulation in human herpesvirus 8 vFLIP-expressing EA hy926 cells. PMID: 30029010
  5. Studied association between nuclear factor kappa beta1/IKK epsilon (NF-kappaB1/IKKepsilon) gene expression and clinical activity in rheumatoid arthritis (RA). Found there were significant differences in NF-kappaB1 and IKKepsilon gene expression (P PMID: 29069487
  6. IKBKE plays a pivotal role in regulating cell proliferation, invasion and epithelial-mesenchymal transition of malignant glioma cells in vitro and in vivo by impacting on the Hippo pathway. PMID: 28548934
  7. Inverse association between IKKepsilon and lymph node metastasis in breast cancer. PMID: 28532474
  8. YPEL5 silencing enhanced the induction of IFNB1 by pattern recognition receptors and phosphorylation of TBK1/IKBKE kinases, whereas co-immunoprecipitation experiments revealed that YPEL5 interacted physically with IKBKE. PMID: 27705791
  9. Virus-activated kinase IKKepsilon phosphorylated YAP at Ser403 and thereby triggered degradation of YAP in lysosomes and, consequently, relief of YAP-mediated inhibition of the cellular antiviral response. PMID: 28481329
  10. We then determined that YAP1 negatively regulates miR-let-7b/i by overexpressing and silencing YAP1 expression. In addition, miR-let-7b/i feedback decreases the expression of IKBKE and YAP1 and suppresses the transportation of YAP1 located in the nucleus. Therefore, the regulatory feedback circuit of IKBKE upward arrow-->YAP1 upward arrow-->miR-let-7b/i downward arrow-->IKBKE upward arrow dictates glioblastoma progress... PMID: 28677425
  11. our findings highlight the functional importance of IKBKE in pancreatic cancer PMID: 28069799
  12. we define a pathobiologic function for IKKepsilon in reprogramming glucose metabolism and driving progression in pancreatic ductal adenocarcinoma PMID: 27923829
  13. demonstrate a key role of TBK1/IKKepsilon in the survival and proliferation of HTLV-1-transformed T cells PMID: 27123832
  14. Data suggest that changes in inhibitor of nuclear factor kappa B kinase subunit epsilon (IKKepsilon) and TANK-binding kinase 1 (TBK1) expression may be involved in the development of intestinal-type gastric cancer. PMID: 27145266
  15. Live NiV infection, but not a recombinant NiV lacking the M protein, reduced the levels of endogenous TRIM6 protein expression. To our knowledge, matrix proteins of paramyxoviruses have never been reported to be involved in innate immune antagonism. We report here a novel mechanism of viral innate immune evasion by targeting TRIM6, IKKepsilon and unanchored polyubiquitin chains. PMID: 27622505
  16. These findings collectively support the conclusion that IKK modulates innate immune signaling cascades via phosphorylating the RIG-I cytosolic sensor, providing a feedback regulatory mechanism. PMID: 26354181
  17. Blunting of NOX2-dependent IKKepsilon oncogenic kinase expression reduces proliferation and viability of human breast cancer cells. PMID: 26177467
  18. The P proteins from the rabies virus street strains 1088 and HCM-9 significantly inhibited I-kappa B kinase (IKK)-inducible IRF-3-dependent IFN-beta promoter activity. PMID: 26647356
  19. IKBKE Upregulation is Positively Associated with Squamous Cell Carcinoma of the Lung In Vivo and Malignant Transformation of Human Bronchial Epithelial Cells In Vitro. PMID: 26025939
  20. DC-SIGN directs adaptive T helper cell type-2immunity to fucose-expressing pathogens via an IKKepsilon-CYLD-dependent signalling pathway leading to Bcl3 activation. PMID: 24867235
  21. these findings reveal that IKBKE-associated cytokine signaling promotes tumorigenicity of immune-driven TNBC and identify a potential therapeutic strategy using clinically available compounds. PMID: 25365225
  22. A role for APPL1 in TLR3/4-dependent TBK1 and IKKepsilon activation in macrophages. PMID: 25780039
  23. The results highlight the importance of the C-terminal region in the functional activity of IKKepsilon in innate immune response.Interestingly, corresponding region and residues are not required for activation of downstream signaling by TBK1. PMID: 24722368
  24. The above-mentioned data suggest that let-7b/i inhibit the invasive ability of glioma cells by directly downregulating IKBKE and indirectly upregulating E-cadherin. PMID: 25656572
  25. IKBKE is a novel regulator that limits chronic inflammation during metabolic disease and atherosclerosis. The pathogenic relevance of IKBKE was indicated by the colocalization with macrophages in tissues and in atherosclerotic plaques. PMID: 25540417
  26. HTLV-1 Tax is recruited into the TBK1/IKKvarepsilon complexes as a scaffolding-adaptor protein that enhances IFN-beta gene expression. PMID: 25531185
  27. Authors identified polymorphisms in the IkBepsilon gene associated with spontaneous hepatitis C resolution in two independent cohorts. PMID: 24224717
  28. in this work, we identified TPCA-1 as a direct dual inhibitor for both IKKs and STAT3, whereas treatment targeting EGFR only could not sufficiently repress NF-kappaB and STAT3 pathways for lung cancers harboring mutant EGFR. PMID: 24401319
  29. probe F2 facilitated the identification of the target spectrum of the two inhibitors confirming many of the previously identified (off-) targets such as AURKA, FLT4-VEGFR3, IKBKE and PDGFRbeta. PMID: 24184958
  30. Using interaction assays, authors found that dengue virus NS2B/3 interacts with the cellular IkappaB kinase epsilon (IKKepsilon). PMID: 24173023
  31. Through interaction with TBK1, phlebovirus nonstructural protein sequester the IKKepsilon and IRF3 into viral inclusion bodies. PMID: 24335286
  32. IKK-epsilon phosphorylated the transcription factor IFN regulatory factor 1 (IRF-1). PMID: 24396068
  33. study provides convinced morphologic evidence that VMAT2 is not present in beta cells PMID: 24155403
  34. we summarize the roles of IKKepsilon and TBK1 in different diseases and outline therapeutic options for modulation of these kinases PMID: 23157677
  35. IKKepsilon increases ERalpha-36 expression and is involved in ERalpha-36 mediated non-genomic estrogen signaling. PMID: 23816933
  36. Ten IKBKE SNVs were associated with systemic lupus erythematosus. PMID: 23535865
  37. IKBKE regulates FOXO3a primarily through phosphorylation of SerS644 PMID: 23691078
  38. Sp1 is required for IL-15 induction by both poly(I:C) and respiratory syncytial virus, a response that also requires NFkappaB2 and IKKepsilon. PMID: 23873932
  39. DDX3 is a scaffolding adaptor that directly facilitates phosphorylation of IRF3 by IKK-epsilon. PMID: 23478265
  40. IkappaBepsilon may be an effective target for HIV latency reactivation in T-cell and macrophage lineages. PMID: 23365428
  41. These experiments demonstrated direct physical interactions of the IKKepsilon and TBK1 kinases with hepatitis C virus NS2. PMID: 23096996
  42. IKBKE is a direct target of STAT3 and is induced by tobacco carcinogens through STAT3 pathway. PMID: 22330135
  43. This work defines TRAF2 phosphorylation to be one key effector of IKKE-induced mammary epithelial cell transformation. PMID: 23007157
  44. these data provide evidence that IKK-epsilon is a key coordinator of invasion and metastasis programs in ovarian cancer PMID: 22942254
  45. NP-IKKepsilon interaction likely plays a crucial role in arenavirus-host interaction. PMID: 22532683
  46. phosphorylation of IRF7 on Ser477 and Ser479 by IKKepsilon or TBK1 is inhibited by KSHV ORF45 PMID: 22787218
  47. deregulation of IKKepsilon plays a pivotal role in the uncontrolled proliferation and malignant invasion of glioma cells PMID: 22552702
  48. identify FOXO3 as a new IKK-epsilon-controlled check-point of IRF activation and regulation of IFN-beta expression, providing new insight into the role of FOXO3 in immune response control. PMID: 22531926
  49. IKBKE and gene expression patterns for other members of the NF-kappaB pathway were not associated with survival, suggesting that IKBKE gene expression may be an independent marker of variation in overall survival PMID: 22266464
  50. Inhibitor of kappaB kinase epsilon (IKK(epsilon)), STAT1, and IFIT2 proteins define novel innate immune effector pathway against West Nile virus infection. PMID: 22065572

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Subcellular Location Cytoplasm. Nucleus. Nucleus, PML body.
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
Tissue Specificity Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
Database Links

HGNC: 14552

OMIM: 605048

KEGG: hsa:9641

STRING: 9606.ENSP00000356087

UniGene: Hs.321045

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