Recombinant Human Keratin, type I cytoskeletal 10(KRT10)

Code CSB-EP012504HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP012504HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KRT10.

  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP012504HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KRT10.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names KRT10
Uniprot No. P13645
Research Area Signal Transduction
Alternative Names BCIE; BIE; CK 10; CK-10; Cytokeratin-10; EHK; K10; K1C10_HUMAN; Keratin 10; Keratin 10 type I; Keratin; Keratin type i cytoskeletal 10; Keratin type I cytoskeletal 59 kDa ; Keratin-10; Keratin10; KPP; KRT10; type I cytoskeletal 10
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-584aa
Target Protein Sequence MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGIFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 62.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Gene References into Functions
  1. The molecular diagnostics by the whole exome sequencing showed a novel de novo (c.1374-2A>C) mutation in the KRT10 gene responsible for the development of IWC (KRT10 defect was confirmed by immunofluorescent study). Concurrently, the m.14484T>C mutation in mitochondrial MTND6 gene (characteristic for Leber's hereditary optic neuropathy or LHON) was detected in patient, his mother and brother PMID: 28944608
  2. KRT10 gene mutation was present in all of the affected individuals, but absent in the five unaffected and 100 ethnically-matched healthy controls. PMID: 27212473
  3. Mutations in the highly conserved helix initiation motif of K10 were associated with mild or severe form of epidermolytic ichthyosis PMID: 26373619
  4. Report genetic/clinical spectrum of KRT10 mutations in keratinopathic ichthyosis. PMID: 26581228
  5. We present an autosomal dominant pedigree with epidermolytic ichthyosis resulting from a new heterozygous missense mutation in keratin 10. PMID: 26338057
  6. recombinant adenovirus-mediated overexpression of KRT10 and PTEN may improve the cisplatin resistance of ovarian cancer in vitro and in vivo PMID: 26125866
  7. findings provide structural insights into phenotypic variation in epidermolytic ichthyosis due to KRT10 mutations PMID: 26176760
  8. The diagnosis of ichthyosis with confetti was confirmed by the identification of 2 previously unreported mutations in intron 6 and exon 7 of KRT10. PMID: 24626314
  9. We present the spectrum of clinical variability of ichthyosis with confetti in 6 patients with confirmed mutations in KRT10. PMID: 25210931
  10. Case Report: extensive postzygotic mosaicism for a novel keratin 10 mutation in epidermolytic ichthyosis. PMID: 24096702
  11. Data indicate that KRT10 is a downstream molecule of PTEN which improves cisplatin-resistance of ovarian cancer cells. PMID: 24434152
  12. Most of the orthokeratotic dysplasia constituent cells were immunopositive for keratin 10, but not for keratins 13, 17 or 19. PMID: 22734720
  13. Molecular mimicry between HSP 65 of Mycobacterium leprae and cytokeratin 10 of the host keratin play the role in pathogenesis of leprosy. PMID: 23121977
  14. Pneumococcal ligands K10, laminin receptor and platelet-activating factor receptor are elevated in aged lungs and contribute to the enhanced susceptibility to pneumonia. PMID: 21615674
  15. mapping and identifying disease-causing mutations in gene encoding KRT10 in ichthyosis with confetti; high frequency of somatic reversion suggests revertant stem cell clones are under positive selection and/or rate of mitotic recombination is elevated PMID: 20798280
  16. Data show that TIMP-1 and cytokeratin-10 were identified as 2 newly synthesized secreted proteins in PMID: 19904223
  17. Expression was altered in oral lichen planus lesions PMID: 19776502
  18. Mutations in codon 156 of K10, i.e. R156S, R156P, R156H were found in epidermolytic hyperkeratosis. PMID: 12234709
  19. cytokeratin 10 molecules were shown to be exposed on the surface of both desquamated nasal epithelial cells and keratinocytes; Staphylococcus aureus clumping factor B (ClfB) was shown to bind to cytokeratin K10 from the desquamated nasal epithelial cells PMID: 12427098
  20. Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha is observed in transgenic mice expressing keratin K10 in the basal layer of the epidermis. PMID: 12566451
  21. normal cornified cell envelope is formed during the process of human epidermal keratinization, even if the suprabasal keratin filament network is disrupted as with this particular K10 mutation, M150T in BCIE PMID: 14705805
  22. cytokeratin 10 may have a role in progression of head and neck squamous cell carcinoma. PMID: 15254692
  23. We demonstrate that a recessive mutation in KRT10 leading to a complete human K10 knockout can cause EHK PMID: 16505000
  24. Candidate autoantigen in chronic, antibiotic-resistant Lyme arthritis. PMID: 16888010
  25. Squamous cell carcinoma (SCC) in mature cystic teratoma (MCT) expressed CK10 less frequently, but CK18 more frequently. SCC in MCT may be derived from metaplastic squamous epithelium. PMID: 17542994
  26. Mild recessive bullous congenital ichthyosiform erythroderma due to a previously unidentified homozygous keratin 10 nonsense mutation. PMID: 18219278
  27. siRNA for PRKD1 resulted in reduction of mRNA levels of PKD1, altered cell phenotype and promotion of keratinocyte differentiation, demonstrated by increased expression of involucrin and K10 mRNAs PMID: 18259765
  28. CK10 alone, or in combination with CK19, can be a novel predictor for poor prognosis of hepatocellular carcinoma patients after curative resection. PMID: 18559605
  29. EHK is an autosomal dominant disorder of keratinization, caused by mutations in either the KRT1 or KRT10 genes. Our patient presents EHK with palmoplantar involvement and KRT10 mutation. PMID: 19443303
  30. premature termination codon of KRT10 maybe involved in recessive epidermolytic hyperkeratosis PMID: 19474805
  31. Infection by HPV may alter the differentiation status of the epidermis, leading to delayed or absent expression of cytokeratin 10. PMID: 19515043

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Involvement in disease Epidermolytic hyperkeratosis (EHK); Ichthyosis annular epidermolytic (AEI); Erythroderma, ichthyosiform, congenital reticular (CRIE)
Protein Families Intermediate filament family
Tissue Specificity Seen in all suprabasal cell layers including stratum corneum.
Database Links

HGNC: 6413

OMIM: 113800

KEGG: hsa:3858

STRING: 9606.ENSP00000269576

UniGene: Hs.99936

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