Recombinant Human Killer cell immunoglobulin-like receptor 3DS1 (KIR3DS1), partial

Code CSB-YP614817HU
MSDS
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Source Yeast
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Code CSB-EP614817HU
MSDS
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Source E.coli
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Code CSB-EP614817HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP614817HU
MSDS
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Source Baculovirus
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Code CSB-MP614817HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIR3DS1
Uniprot No.
Alternative Names
KIR3DS1; NKAT10Killer cell immunoglobulin-like receptor 3DS1; MHC class I NK cell receptor; Natural killer-associated transcript 10; NKAT-10
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor on natural killer (NK) cells for MHC class I molecules. Upon interaction with peptide-free HLA-F open conformer, triggers NK cell degranulation and anti-viral cytokine production.
Gene References into Functions
  1. interactions between KIR3DS1 and HLA-F contribute to NK cell-mediated control of HCV. PMID: 30031767
  2. The combination of KIR3DS1 with homozygosity for HLA-Bw4 alleles (the putative ligand for KIR3DS1) was significantly less frequent in the HIV-1 infected group than in the control group PMID: 29664957
  3. Furthermore, the KIR3DS1 + HLA-Bw4, KIR3DS1 + HLA-Bw4 (Iso80) , and KIR3DS1 + HLA-A Bw4 genotypes were significantly more common in recovered individuals than both healthy control and patient groups. PMID: 29032460
  4. KIR3DS1*01301, 3DL1*00401, and 3DL1*00402 showed susceptibility and elevated mRNA expression and is associated with antibody mediated acute rejection and chronic rejection. PMID: 28942035
  5. Study assessed which of the TB01 encoded KIR gene products contributed to Natural Killer cell responsiveness; of the TB01 KIR gene products examined, only KIR3DS1 conferred responsiveness to HLA-null stimulation, demonstrating its ligation can activate ex vivo Natural Killer cells. PMID: 28938026
  6. genetic polymorphism is not related to acute myeloid leukemia in Chinese population PMID: 25636577
  7. The data from this study contribute novel insight into how KIR3DS1-specific polymorphisms in the extracellular region impact KIR3DL1 surface expression, ligand binding, and inhibitory function. PMID: 26109640
  8. Data suggest that low level of activating KIR3DS1 and its combination with HLA-B Bw4Ile80 ligand might have an influence on the susceptibility to tuberculosis (TB) in Lur population of Iran. PMID: 22426166
  9. KIR3DS1, in addition to HLA-B27, may play an important independent role in the pathogenesis of ankylosing spondylitis in the Chinese population. PMID: 22744805
  10. Results show that the KIR3DS1 genotype has a positive effect on HCV viral clearance during the first weeks of Peg-IFN/RBV treatment in HCV/HCV co-infected patients bearing genotype 1, and higher RVR and SVR rates. PMID: 23613999
  11. results suggest that the sole presence of KIR3DS1 could have a protective role in HIV-1 infection in highly exposed and persistently seronegative individuals PMID: 23789883
  12. There is evidence of the association of activating KIR genotypes with an increased risk for autoimmune disease PMID: 21779711
  13. Our results that KIR2DL5 and KIR3DS1 may have a predisposing role in multiple sclerosis PMID: 21665278
  14. These data provide the first evidence for the direct binding of KIR3DS1(+) cells to HLA-Bw4 and highlight the key role for position 138 in determining ligand specificity of KIR3DS1 PMID: 21804024
  15. The most significant difference in PFS observed in multiple myeloma patients after autologous stem cell transplantation was with those with GR KIR3DS1(+) in whom HLA-Bw4, the ligand for the corresponding inhibitory receptor KIR3DL1, was missing PMID: 20562327
  16. The increased frequency of the KIR3DS1*013 allele in patients with amnkylosing spondylitis is clearly independent of the presence of the HLA-Bw4I80 epitope. PMID: 20131260
  17. Compared with KIR3DS1(-) donors, donor KIR3DS1 was associated with lower-grade II-IV acute graft-versus-host disease. PMID: 20124216
  18. Carriage of activating 3ds1 was increased in patients with herpes virus RDs. PMID: 17592337
  19. Sequencing analysis identified a variant with a complex deletion/substitution mutation in exon 4 (which encodes the D1 extracellular domain), resulting in a premature stop codon and might affect its expression and activating capacity PMID: 17687550
  20. Variation at the KIR locus influences the effectiveness of NK cell activity in the containment of viral replication. PMID: 18025129
  21. We observed that possessing KIR3DS1 was associated with higher NK cell effector functions in early HIV-1 disease, despite the absence of HLA Bw4Ile80, a putative ligand of KIR3DS1. PMID: 18305035
  22. KIR3DS1 expression on NK cells can be induced after exposure to stimulator cells PMID: 19454667

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Immunoglobulin superfamily
Tissue Specificity
Expressed in NK and T-cell lines but not in B-lymphoblastoid cell lines or in a colon carcinoma cell line.
Database Links

HGNC: 6340

OMIM: 604946

KEGG: hsa:3813

STRING: 9606.ENSP00000375608

UniGene: Hs.645228

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