Recombinant Human Kinesin-1 heavy chain (KIF5B), partial

Code CSB-EP012342HU2
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Target Names
KIF5B
Uniprot No.
Research Area
Cancer
Alternative Names
(Conventional kinesin heavy chain)(Ubiquitous kinesin heavy chain)(UKHC)
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-431aa
Target Protein Sequence
MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
54.9 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation. Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells.
Gene References into Functions
  1. The KIF5B-MET variant was demonstrated to have an oncogenic function in cancer cells. PMID: 30015159
  2. the discovery of kinesore, a small molecule that in vitro inhibits kinesin-1 interactions with short linear peptide motifs found in organelle-specific cargo adaptors, yet activates kinesin-1's function of controlling microtubule dynamics in cells, demonstrating that these functions are mechanistically coupled. PMID: 29229862
  3. HIV-1 did not stimulate widespread FEZ1 phosphorylation but, instead, bound microtubule (MT) affinity-regulating kinase 2 (MARK2) to stimulate FEZ1 phosphorylation on viral cores. PMID: 28930676
  4. Study demonstrate that the kinesin and kinase domains of KIF5B-RET act together to establish an emergent microtubule and RAB-vesicle-dependent RET-SRC-EGFR-FGFR signaling hub. Study demonstrate that drugs designed to inhibit RET alone work poorly in KIF5B-RET-transformed cells. PMID: 28877471
  5. In particular, counteracting forces between minus-end-directed kinesin-14 and plus-end-directed kinesin-5 motors have recently been implicated in the regulation of microtubule nucleation. PMID: 28668932
  6. The studies describing the interplay between kinesin-1 and cytoplasmic dynein in the translocation of microtubules are discussed. PMID: 28284467
  7. Utilizing novel electron cryomicroscopy methods, the authors solved structures of microtubule-attached, dimeric kinesin bound to an ATP analog. They find that under these conditions, the kinesin dimer can attach to the microtubule with either one or two motor domains. PMID: 28504639
  8. cargo recognition and concurrent activation of kinesin-1 proceed in hierarchical stepwise fashion driven by a dynamic network of inter- and intra-molecular interactions. PMID: 28302907
  9. BORC and Arl8 function upstream of two structurally distinct kinesin types: kinesin-1 (KIF5B) and kinesin-3 (KIF1Bbeta and KIF1A). PMID: 27851960
  10. KIF5B gene rearrangement plays a role in the pathogenesis of papillary thyroid cancer. PMID: 28911147
  11. compared to those of brain, on MCF7 microtubules, kinesin-1's processivity is significantly reduced although its velocity is only slightly altered PMID: 25450690
  12. these observations identify a previously unappreciated role for KIF5B in mediating the Nup358 dependent nuclear import of the HIV-1 viral genome during infection. PMID: 27327622
  13. For Fat3, the Kif5-ID is regulated by alternative splicing, and the timecourse of splicing suggests that the distribution of Fat3 may switch between early and later stages of retinal development. In contrast, P75NTR binding to Kif5B is enhanced by tyrosine phosphorylation and thus has the potential to be dynamically regulated on a more rapid time scale PMID: 27788242
  14. Data suggest that light-chain subunits/fragments of kinesin-1 inhibit the pathological aggregation of amyloid(beta). PMID: 26991731
  15. In this work, we performed normal mode analysis with elastic network model using different conformation of kinesin to determine the effect of tail binding. PMID: 25676575
  16. KIF5B is essential for Ostm1 intracellular dispersion. PMID: 26598607
  17. differences between the structural kinetics of Eg5 and kinesin-1 yield insights into how these two motors adapt their enzymologies for their distinct functions PMID: 26627252
  18. data suggest one-step screening platform for KIF5B-RET as well as EGFR, K-RAS, ALK oncogenic mutations be necessary for lung adenocarcinoma patients PMID: 26268359
  19. Kinesin-1 is primarily front-head gated, and its neck linker length is tuned to enhance unidirectional processivity and velocity. PMID: 25902401
  20. the front head of a kinesin dimer is gated by the backward orientation of its neck linker until the rear head releases from the microtubule PMID: 25818289
  21. Myo1c significantly increases the frequency of kinesin-1-driven microtubule-based runs that begin at actin/microtubule intersections. The actin-binding protein tropomyosin 2 abolishes Myo1c-specific effects on both run initiation and run termination. PMID: 25660542
  22. A novel role of KIF5B was shown in the spatial regulation of Cdo-BNIP-2-p38MAPK signaling and discloses a previously unappreciated linkage between the intracellular transporting system and myogenesis regulation. PMID: 25378581
  23. This study has provided a comprehensive structural and dynamic picture of kinesin's major ATPase states. PMID: 25537000
  24. nesprin-dependent recruitment of kinesin-1 to the nuclear envelope through the interaction of a conserved LEWD motif with kinesin light chain might be a general mechanism for cell-type-specific nuclear positioning during development. PMID: 25516977
  25. Authors find that pharmacological or small interfering RNA (siRNA)-mediated inhibition of cytoplasmic dynein or the kinesin 1 heavy chain KIF5B delays HIV-1 uncoating. PMID: 25231297
  26. Our data suggest that KIF5B-RET promotes the cell growth and tumorigenicity of non-small cell lung cancers through multilevel activation of STAT3 signaling, providing possible strategies for the treatment of KIF5B-RET positive lung cancers. PMID: 25047660
  27. Kinesin-1 transports c-MYC for proteasomal degradation in the cytoplasm. PMID: 24821626
  28. Anti-oncogenic miR-203 had a pivotal role in melanoma through reducing melanosome transport and promoting melanogenesis by targeting kif5b and through negative regulation of the CREB1/MITF/Rab27a pathway. PMID: 23884313
  29. when cargo is transported by both dynein and phosphorylated kinesin, a common occurrence in the cell, there may be a bias that favors motion toward the minus-end of microtubules. PMID: 24072715
  30. Patients without KIF5B-RET fusions had a better prognosis than those with KIF5B-RET fusions (median survival, 52.6 months vs 21.0 months). PMID: 23378251
  31. Study found the secretion of calu-1/2-EGFP required microtubule integrity, and that calu-1/2-EGFP-containing vesicles were transported by the motor proteins Kif5b and cytoplasmic dynein. PMID: 22514732
  32. Nesca directly binds KIF5B, kinesin light-chain and syntaxin-1 PMID: 22404429
  33. Expression of exogenous KIF5B-RET, induced morphological transformation and anchorage-independent growth of NIH3T3 fibroblasts PMID: 22327624
  34. Data indicate that mutant KIF5B kinesin-1 binds ATP similarly in the presence of either metal ion, but its ATP hydrolysis activity is greatly diminished in the presence of Mg(2+). PMID: 22198464
  35. Arl8 and SKIP are required for lysosomes to distribute away from the microtubule-organizing center. We identify two kinesin light chain binding motifs in SKIP that are required for lysosomes to accumulate kinesin-1 and redistribute to the cell periphery. PMID: 22172677
  36. Kinesin-1-mediated capsid disassembly and disruption of the nuclear pore complex promote virus infection. PMID: 21925109
  37. findings show a bipartite tryptophan-based motif in vaccinia virus A36 is required for kinesin-1-dependent transport of the virus; bioinformatic analysis reveals that related bipartite tryptophan-based motifs are present in over 450 human proteins PMID: 21915095
  38. The conventional kinesin-microtubule binding free energy is dominated by van der Waals interactions and electrostatic interactions. PMID: 21910419
  39. The discovery of the novel KIF5B-ALK variant further consolidated the role of aberrant anaplastic lymphoma kinase signaling in lung carcinogenesis. PMID: 21656749
  40. the interaction between the kinesin-1 head and its regulatory tail domain PMID: 19348763
  41. a kinesin-1 tail fragment associates with microtubules with submicromolar affinity and binding is largely electrostatic PMID: 20071331
  42. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events. PMID: 19913510
  43. Na,K-ATPase traffic toward the plasma membrane in alveolar epithelial cells is driven by kinesin-1, where KLC2 is the isoform regulating this process. PMID: 19773350
  44. links neurofibromin and merlin in a common cellular pathway of neurofibromatosis PMID: 12191989
  45. With residues 814-963 of human ubiquitous kinesin heavy chain (uKHC) used as bait, a synaptosome-associated protein of 25 kDa (SNAP25) has been identified as a kinesin-binding protein in a human brain cDNA library. PMID: 12475239
  46. Results identify the ribosome receptor, p180, as a binding partner of the kinesin heavy chain isoform KIF5B. PMID: 15184079
  47. CRMP-2 transports the Sra-1/WAVE1 complex to axons in a kinesin-1-dependent manner and thereby regulates axon outgrowth and formation PMID: 16260607
  48. Demonstrates the utility of screening for PDGFRA kinase domain overexpression in patients with IHES and has identified KIF5B as a third PDGFRA fusion partner in chronic myeloproliferative disorders. PMID: 16498388
  49. binding of the kinesin-binding domain of RanBP2 to KIF5B and KIF5C determines mitochondria localization and function PMID: 17887960
  50. We demonstrate that KIF5B mediates post-Golgi transport of an apical protein in epithelial cells, but only after polarity has developed. PMID: 17925227

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Subcellular Location
Cytoplasm, cytoskeleton. Cytolytic granule membrane. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin subfamily
Database Links

HGNC: 6324

OMIM: 602809

KEGG: hsa:3799

STRING: 9606.ENSP00000307078

UniGene: Hs.327736

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