Product Details

Uniprot No.

P33176

Target Names

KIF5B

Alternative Names

Kinesin-1 heavy chain (Conventional kinesin heavy chain) (Ubiquitous kinesin heavy chain) (UKHC), KIF5B, KNS KNS1

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human KIF5B protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10H1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

KIF5B, also known as kinesin-1 heavy chain or ubiquitous kinesin heavy chain, serves as a critical molecular motor protein responsible for anterograde transport of organelles, vesicles, and protein complexes along microtubules. This ATP-dependent motor plays essential roles in cellular processes including mitochondrial distribution, lysosomal trafficking, and neuronal transport, making it a significant target for researchers investigating intracellular logistics, neurodegenerative mechanisms, and cancer cell biology.

This recombinant monoclonal antibody, clone 10H1, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human KIF5B, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms robust detection in human cell lines including HeLa, A431, and Jurkat, with cross-species reactivity extending to mouse and rat tissues, including brain and heart lysates. The observed band at approximately 120 kDa runs slightly higher than the predicted 110 kDa molecular weight, consistent with post-translational modifications (e.g., N-glycosylation) commonly observed with this protein. Immunohistochemistry performance has been validated in human breast cancer and stomach tissue sections using citrate-based antigen retrieval, while immunofluorescence staining in HepG2 cells reveals characteristic cytoplasmic distribution patterns. Flow cytometry applications have been confirmed using MCF-7 cells with clear positive signal separation.

Whether investigating motor protein dysfunction in neurological disease models, examining organelle trafficking in cancer progression, or characterizing cytoskeletal dynamics, this antibody provides a reliable tool for KIF5B detection across diverse experimental contexts and species.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation. Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells.

Gene References into Functions

The KIF5B-MET variant was demonstrated to have an oncogenic function in cancer cells. PMID: 30015159
the discovery of kinesore, a small molecule that in vitro inhibits kinesin-1 interactions with short linear peptide motifs found in organelle-specific cargo adaptors, yet activates kinesin-1's function of controlling microtubule dynamics in cells, demonstrating that these functions are mechanistically coupled. PMID: 29229862
HIV-1 did not stimulate widespread FEZ1 phosphorylation but, instead, bound microtubule (MT) affinity-regulating kinase 2 (MARK2) to stimulate FEZ1 phosphorylation on viral cores. PMID: 28930676
Study demonstrate that the kinesin and kinase domains of KIF5B-RET act together to establish an emergent microtubule and RAB-vesicle-dependent RET-SRC-EGFR-FGFR signaling hub. Study demonstrate that drugs designed to inhibit RET alone work poorly in KIF5B-RET-transformed cells. PMID: 28877471
In particular, counteracting forces between minus-end-directed kinesin-14 and plus-end-directed kinesin-5 motors have recently been implicated in the regulation of microtubule nucleation. PMID: 28668932
The studies describing the interplay between kinesin-1 and cytoplasmic dynein in the translocation of microtubules are discussed. PMID: 28284467
Utilizing novel electron cryomicroscopy methods, the authors solved structures of microtubule-attached, dimeric kinesin bound to an ATP analog. They find that under these conditions, the kinesin dimer can attach to the microtubule with either one or two motor domains. PMID: 28504639
cargo recognition and concurrent activation of kinesin-1 proceed in hierarchical stepwise fashion driven by a dynamic network of inter- and intra-molecular interactions. PMID: 28302907
BORC and Arl8 function upstream of two structurally distinct kinesin types: kinesin-1 (KIF5B) and kinesin-3 (KIF1Bbeta and KIF1A). PMID: 27851960
KIF5B gene rearrangement plays a role in the pathogenesis of papillary thyroid cancer. PMID: 28911147
compared to those of brain, on MCF7 microtubules, kinesin-1's processivity is significantly reduced although its velocity is only slightly altered PMID: 25450690
these observations identify a previously unappreciated role for KIF5B in mediating the Nup358 dependent nuclear import of the HIV-1 viral genome during infection. PMID: 27327622
For Fat3, the Kif5-ID is regulated by alternative splicing, and the timecourse of splicing suggests that the distribution of Fat3 may switch between early and later stages of retinal development. In contrast, P75NTR binding to Kif5B is enhanced by tyrosine phosphorylation and thus has the potential to be dynamically regulated on a more rapid time scale PMID: 27788242
Data suggest that light-chain subunits/fragments of kinesin-1 inhibit the pathological aggregation of amyloid(beta). PMID: 26991731
In this work, we performed normal mode analysis with elastic network model using different conformation of kinesin to determine the effect of tail binding. PMID: 25676575
KIF5B is essential for Ostm1 intracellular dispersion. PMID: 26598607
differences between the structural kinetics of Eg5 and kinesin-1 yield insights into how these two motors adapt their enzymologies for their distinct functions PMID: 26627252
data suggest one-step screening platform for KIF5B-RET as well as EGFR, K-RAS, ALK oncogenic mutations be necessary for lung adenocarcinoma patients PMID: 26268359
Kinesin-1 is primarily front-head gated, and its neck linker length is tuned to enhance unidirectional processivity and velocity. PMID: 25902401
the front head of a kinesin dimer is gated by the backward orientation of its neck linker until the rear head releases from the microtubule PMID: 25818289
Myo1c significantly increases the frequency of kinesin-1-driven microtubule-based runs that begin at actin/microtubule intersections. The actin-binding protein tropomyosin 2 abolishes Myo1c-specific effects on both run initiation and run termination. PMID: 25660542
A novel role of KIF5B was shown in the spatial regulation of Cdo-BNIP-2-p38MAPK signaling and discloses a previously unappreciated linkage between the intracellular transporting system and myogenesis regulation. PMID: 25378581
This study has provided a comprehensive structural and dynamic picture of kinesin's major ATPase states. PMID: 25537000
nesprin-dependent recruitment of kinesin-1 to the nuclear envelope through the interaction of a conserved LEWD motif with kinesin light chain might be a general mechanism for cell-type-specific nuclear positioning during development. PMID: 25516977
Authors find that pharmacological or small interfering RNA (siRNA)-mediated inhibition of cytoplasmic dynein or the kinesin 1 heavy chain KIF5B delays HIV-1 uncoating. PMID: 25231297
Our data suggest that KIF5B-RET promotes the cell growth and tumorigenicity of non-small cell lung cancers through multilevel activation of STAT3 signaling, providing possible strategies for the treatment of KIF5B-RET positive lung cancers. PMID: 25047660
Kinesin-1 transports c-MYC for proteasomal degradation in the cytoplasm. PMID: 24821626
Anti-oncogenic miR-203 had a pivotal role in melanoma through reducing melanosome transport and promoting melanogenesis by targeting kif5b and through negative regulation of the CREB1/MITF/Rab27a pathway. PMID: 23884313
when cargo is transported by both dynein and phosphorylated kinesin, a common occurrence in the cell, there may be a bias that favors motion toward the minus-end of microtubules. PMID: 24072715
Patients without KIF5B-RET fusions had a better prognosis than those with KIF5B-RET fusions (median survival, 52.6 months vs 21.0 months). PMID: 23378251
Study found the secretion of calu-1/2-EGFP required microtubule integrity, and that calu-1/2-EGFP-containing vesicles were transported by the motor proteins Kif5b and cytoplasmic dynein. PMID: 22514732
Nesca directly binds KIF5B, kinesin light-chain and syntaxin-1 PMID: 22404429
Expression of exogenous KIF5B-RET, induced morphological transformation and anchorage-independent growth of NIH3T3 fibroblasts PMID: 22327624
Data indicate that mutant KIF5B kinesin-1 binds ATP similarly in the presence of either metal ion, but its ATP hydrolysis activity is greatly diminished in the presence of Mg(2+). PMID: 22198464
Arl8 and SKIP are required for lysosomes to distribute away from the microtubule-organizing center. We identify two kinesin light chain binding motifs in SKIP that are required for lysosomes to accumulate kinesin-1 and redistribute to the cell periphery. PMID: 22172677
Kinesin-1-mediated capsid disassembly and disruption of the nuclear pore complex promote virus infection. PMID: 21925109
findings show a bipartite tryptophan-based motif in vaccinia virus A36 is required for kinesin-1-dependent transport of the virus; bioinformatic analysis reveals that related bipartite tryptophan-based motifs are present in over 450 human proteins PMID: 21915095
The conventional kinesin-microtubule binding free energy is dominated by van der Waals interactions and electrostatic interactions. PMID: 21910419
The discovery of the novel KIF5B-ALK variant further consolidated the role of aberrant anaplastic lymphoma kinase signaling in lung carcinogenesis. PMID: 21656749
the interaction between the kinesin-1 head and its regulatory tail domain PMID: 19348763
a kinesin-1 tail fragment associates with microtubules with submicromolar affinity and binding is largely electrostatic PMID: 20071331
This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events. PMID: 19913510
Na,K-ATPase traffic toward the plasma membrane in alveolar epithelial cells is driven by kinesin-1, where KLC2 is the isoform regulating this process. PMID: 19773350
links neurofibromin and merlin in a common cellular pathway of neurofibromatosis PMID: 12191989
With residues 814-963 of human ubiquitous kinesin heavy chain (uKHC) used as bait, a synaptosome-associated protein of 25 kDa (SNAP25) has been identified as a kinesin-binding protein in a human brain cDNA library. PMID: 12475239
Results identify the ribosome receptor, p180, as a binding partner of the kinesin heavy chain isoform KIF5B. PMID: 15184079
CRMP-2 transports the Sra-1/WAVE1 complex to axons in a kinesin-1-dependent manner and thereby regulates axon outgrowth and formation PMID: 16260607
Demonstrates the utility of screening for PDGFRA kinase domain overexpression in patients with IHES and has identified KIF5B as a third PDGFRA fusion partner in chronic myeloproliferative disorders. PMID: 16498388
binding of the kinesin-binding domain of RanBP2 to KIF5B and KIF5C determines mitochondria localization and function PMID: 17887960
We demonstrate that KIF5B mediates post-Golgi transport of an apical protein in epithelial cells, but only after polarity has developed. PMID: 17925227

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Subcellular Location

Cytoplasm, cytoskeleton. Cytolytic granule membrane. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin subfamily

Database Links

HGNC: 6324

OMIM: 602809

KEGG: hsa:3799

STRING: 9606.ENSP00000307078

UniGene: Hs.327736

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Code	CSB-RA266593A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), A431 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg), Mouse heart tissue lysate(30µg) All lanes:KIF5B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 110 kDa Observed band size: 120 kDa Exposure time：2min IHC image of CSB-RA266593A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA266593A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA266593A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA266593A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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KIF5B Recombinant Monoclonal Antibody

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