Recombinant Human Kinesin-like protein KIF1C (KIF1C), partial

Code CSB-YP012320HU
MSDS
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Source Yeast
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Code CSB-EP012320HU
MSDS
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Source E.coli
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Code CSB-EP012320HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012320HU
MSDS
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Source Baculovirus
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Code CSB-MP012320HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIF1C
Uniprot No.
Alternative Names
Kif1c; KIF1C_HUMAN; Kinesin family member 1C; Kinesin-like protein KIF1C; LTXS1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Motor required for the retrograde transport of Golgi vesicles to the endoplasmic reticulum. Has a microtubule plus end-directed motility.
Gene References into Functions
  1. Hereditary spastic paraplegia (HSP) patients with KIF1C mutations may present with cerebellar signs and pyramidal findings may emerge later, therefore complicated HSP should be considered in the differential diagnosis of unidentified cases with cerebellar dysfunction. PMID: 29544888
  2. Rab6A binding to KIF1C's motor domain represents an entirely new mode of regulation for a kinesin motor, and likely has important consequences for KIF1C's cellular functions. PMID: 25821985
  3. KIF1C translocation to the cell periphery intensifies and KIF1C accumulates both in the proximity of peripheral microtubules that show enrichment for the plus-tip-associated proteins CLASPs and around podosomes. PMID: 25344256
  4. Microtubule acetylation influences the subcellular distribution of vesicles associated with the kinesin KIF1C, as well as their directionality, velocity and run length. PMID: 25151635
  5. Nonsense and missense mutations in the KIF1C gene associated with hereditary spastic paraparesis and cerebellar dysfunction. PMID: 24319291
  6. Identification of KIF1C as an hereditary spastic paraplegia gene supports the key role of intracellular trafficking processes in the pathogenesis of hereditary axonopathies. PMID: 24808017
  7. findings show that the microtubule motor Kif1C contributes to persistent cell migration primarily through stabilization of an extended cell rearKif1C-mediated transport of alpha5beta1-integrins is required for the proper maturation of trailing focal adhesions and resistance to tail retraction PMID: 23237952
  8. Hairpin RNA-based depletion of KIF1C resulted in decreased podosome dynamics and ultimately in podosome deficiency. PMID: 16554367

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Involvement in disease
Spastic ataxia 2, autosomal recessive (SPAX2)
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Unc-104 subfamily
Tissue Specificity
Expressed in all tissues examined, with most abundant expression in heart and skeletal muscle.
Database Links

HGNC: 6317

OMIM: 603060

KEGG: hsa:10749

STRING: 9606.ENSP00000320821

UniGene: Hs.435120

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