Product Details

Purity

>85% (SDS-PAGE)

Target Names

IL4I1

Uniprot No.

Q96RQ9

Alternative Names

IL4I1; FIG1; UNQ636/PRO1265; L-amino-acid oxidase; LAAO; LAO; EC 1.4.3.2; Interleukin-4-induced protein 1; IL4-induced protein 1; Protein Fig-1; hFIG1

Species

Homo sapiens (Human)

Expression Region

22-567

Target Protein Sequence

QDWKAERSQ DPFEKCMQDP DYEQLLKVVT WGLNRTLKPQ RVIVVGAGVA GLVAAKVLSD AGHKVTILEA DNRIGGRIFT YRDQNTGWIG ELGAMRMPSS HRILHKLCQG LGLNLTKFTQ YDKNTWTEVH EVKLRNYVVE KVPEKLGYAL RPQEKGHSPE DIYQMALNQA LKDLKALGCR KAMKKFERHT LLEYLLGEGN LSRPAVQLLG DVMSEDGFFY LSFAEALRAH SCLSDRLQYS RIVGGWDLLP RALLSSLSGL VLLNAPVVAM TQGPHDVHVQ IETSPPARNL KVLKADVVLL TASGPAVKRI TFSPPLPRHM QEALRRLHYV PATKVFLSFR RPFWREEHIE GGHSNTDRPS RMIFYPPPRE GALLLASYTW SDAAAAFAGL SREEALRLAL DDVAALHGPV VRQLWDGTGV VKRWAEDQHS QGGFVVQPPA LWQTEKDDWT VPYGRIYFAG EHTAYPHGWV ETAVKSALRA AIKINSRKGP ASDTASPEGH ASDMEGQGHV HGVASSPSHD LAKEEGSHPP VQGQLSLQNT THTRTSH

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.

Gene References into Functions

the presence of IL4I1 during T-cell activation decreases early signaling events downstream of t-cell receptor stimulation, resulting in global T-cell inhibition which is more pronounced when there is CD28 costimulation. PMID: 28891065
Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
reviewed SNPs of the IL4I1 isoform 1, which is expressed in lymphoid tissue; the N92D SNP leads to a hyperactive enzyme, while the R102G mutation is hypomorphic; show that IL4I1 activity is not only directed against phenylalanine, as initially described, but also at a lower level against arginine PMID: 26673964
The immunosuppressive enzyme IL4I1 expressed differentially in human induced Aiolos+, but not natural Helios+, FOXP3+ Treg cells. PMID: 25446972
IL4I1 upregulation in human Th17 cells limits their TCR-mediated expansion not only by blocking the molecular pathway involved in the activation of the IL-2 promoter, but also by maintaining high levels of Tob1, which impairs entry into the cell cycle. PMID: 24307243
IL4I1 plays a distinct role compared to other antibacterial enzymes produced by mononuclear phagocytes. PMID: 23355881
participates in the downregulation of Th1 inflammation PMID: 20683900
genomic structure, cDNA sequence, chromosome location and RNA expression in immune tissues PMID: 12031486
is activated in primary mediastinal large B-cell lymphoma, which might be due to a constitutive activation of a cytokine signaling pathway PMID: 12446450
hIL4I1 inhibited the proliferation of CD3-stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells. In contrast, memory T cells were more strongly affected by hIL4I1 and its catabolite H(2)O(2) than naive T cells PMID: 17356132
strong IL4I1 expression is associated with B-cell lymphomas. PMID: 19436310

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Subcellular Location

Secreted. Lysosome. Cytoplasmic vesicle, secretory vesicle, acrosome.

Protein Families

Flavin monoamine oxidase family, FIG1 subfamily

Tissue Specificity

Primarily found in immune tissues, with the highest expression in lymph nodes and spleen. Present in germinal center macrophages and inflammatory myeloid cells and antigen-presenting cells (at protein level). Also present in spermatozoa (at protein level)

Database Links

HGNC: 19094

OMIM: 609742

KEGG: hsa:259307

STRING: 9606.ENSP00000342557

UniGene: Hs.574492

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Recombinant Human L-amino-acid oxidase (IL4I1)

Product Details

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Target Background

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