Recombinant Human L-xylulose reductase (DCXR)

Code CSB-EP772022HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
DCXR
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Carbonyl reductase 2; Carbonyl reductase II; DCR; Dcxr; DCXR_HUMAN; Dicarbonyl and L xylulose reductase; Dicarbonyl L xylulose reductase; Dicarbonyl/ L xylulose reductase; Dicarbonyl/L-xylulose reductase; HCR2; HCRII; Human carbonyl reductase 2; kiDCR; Kidney dicarbonyl reductase; L xylulose reductase; L-xylulose reductase; P34H; PNTSU; SDR20C1; Short chain dehydrogenase/reductase family 20C member 1; Sperm surface protein P34H; XR
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-244aa
Target Protein Sequence
MELFLAGRRVLVTGAGKGIGRGTVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALGSVGPVDLLVNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGAIVNVSSQCSQRAVTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPTVVMTSMGQATWSDPHKAKTMLNRIPLGKFAEVEHVVNAILFLLSDRSGMTTGSTLPVEGGFWAC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
52.9kDa
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
Gene References into Functions
  1. Ureaplasma urealyticum infection may affect the level of P34H protein expression on spermatozoa PMID: 28012250
  2. identification of DCXR as an enzyme activity mediating chemical redox cycling suggests that it may be important in generating cytotoxic reactive oxygen species in the lung PMID: 28595002
  3. Low expression of DCXR is associated with hepatocellular carcinoma. PMID: 27658779
  4. EGCG is a potential inhibitor to human DCXR. PMID: 23661708
  5. combined frequency of the two mutant DCXR alleles in 1,067 Ashkenazi Jewish controls was 0.0173, suggesting a pentosuria frequency of approximately one in 3,300 in this population PMID: 22042873
  6. highly expressed in kidney and liver PMID: 11882650
  7. crystallization and preliminary crystallographic analysis PMID: 12136162
  8. structure of the tetrameric form of human L-Xylulose reductase PMID: 15906319
  9. Our results demonstrate that the proportion of positive P34H cases that produced embryos in vitro clearly differs from cases with undetectable levels of P34H (P<.001). PMID: 16616743
  10. These results suggested that altered HCR2 expression might play roles in the carcinogenesis and progression of hepatocellular carcinoma. PMID: 16847567
  11. A significant proportion of men investigated for male infertility may be epididymal protein P34H deficient. PMID: 17434498
  12. Decreased membranous expression of DCXR with altered subcellular localization appears to be associated with malignant progression of melanocytic lesions in human skin. PMID: 17576332
  13. DCXR trranfected into mice may function in the removal of renal alpha-dicarbonyl compounds under oxidative circumstances, but it is not sufficient to suppress acute renal fibrosis. PMID: 18079483
  14. DCXR overexpression has the potential to be an additional useful biomarker for prostate cancer. PMID: 18086765
  15. The action of human XR may be regulated by cellular redox conditions through reversible disulfide-bond formation and by S-cysteinylation. PMID: 19337691

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Involvement in disease
Pentosuria (PNTSU)
Subcellular Location
Membrane; Peripheral membrane protein.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family
Tissue Specificity
Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis.
Database Links

HGNC: 18985

OMIM: 260800

KEGG: hsa:51181

STRING: 9606.ENSP00000303356

UniGene: Hs.9857

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