Recombinant Human Lysosomal Pro-X carboxypeptidase(PRCP)

Code CSB-YP018636HU
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Source Yeast
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Code CSB-EP018636HU
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Source E.coli
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Code CSB-EP018636HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018636HU
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Source Baculovirus
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Code CSB-MP018636HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names PRCP
Uniprot No. P42785
Alternative Names Angiotensinase C; HUMPCP ; Lysosomal carboxypeptidase C; Lysosomal Pro X carboxypeptidase; Lysosomal Pro-X carboxypeptidase; MGC2202; PCP ; PCP_HUMAN; PRCP; Proline carboxypeptidase; Prolylcarboxypeptidase (angiotensinase C); Prolylcarboxypeptidase; prolylcarboxypeptidase isoform 1 preproprotein
Species Homo sapiens (Human)
Expression Region 46-496
Target Protein Sequence KNYSV LYFQQKVDHF GFNTVKTFNQ RYLVADKYWK KNGGSILFYT GNEGDIIWFC NNTGFMWDVA EELKAMLVFA EHRYYGESLP FGDNSFKDSR HLNFLTSEQA LADFAELIKH LKRTIPGAEN QPVIAIGGSY GGMLAAWFRM KYPHMVVGAL AASAPIWQFE DLVPCGVFMK IVTTDFRKSG PHCSESIHRS WDAINRLSNT GSGLQWLTGA LHLCSPLTSQ DIQHLKDWIS ETWVNLAMVD YPYASNFLQP LPAWPIKVVC QYLKNPNVSD SLLLQNIFQA LNVYYNYSGQ VKCLNISETA TSSLGTLGWS YQACTEVVMP FCTNGVDDMF EPHSWNLKEL SDDCFQQWGV RPRPSWITTM YGGKNISSHT NIVFSNGELD PWSGGGVTKD ITDTLVAVTI SEGAHHLDLR TKNALDPMSV LLARSLEVRH MKNWIRDFYD SAGKQH
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
Gene References into Functions
  1. For the first time, PRCP is identified as an apelin-cleaving enzyme. PMID: 27449720
  2. The decrease in PRCP levels in the first 24 h after stroke onset is associated with stroke severity and an unfavourable short-term stroke outcome PMID: 25370794
  3. PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation. PMID: 25324000
  4. The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population. PMID: 22679278
  5. PRCP regulates cell growth, angiogenesis, and the response to vascular injury PMID: 23744584
  6. analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors PMID: 22079761
  7. PRCP as a resistance factor for 4OHTAM resistance in estrogen receptor-positive breast cancer cells. PMID: 21087932
  8. A single peptide, with the sequence YPRPIHPA, as a novel substrate for PRCP in human cerebrospinal fluid. PMID: 20517885
  9. Purified PrCP yielded crystals belonging to space group R32, with unit-cell parameters a = b = 181.14, c = 240.13 A, that diffracted to better than 2.8 A resolution. PMID: 20516604
  10. A structure-based alignment with the previously undescribed structure of DPP7 illuminates the mechanism of orthogonal substrate specificity of PRCP and DPP7. PMID: 20540760
  11. Data suggest that the E112D polymorphism in the PRCP gene may be a useful genetic marker to predict the antihypertensive effect of short-term benazepril treatment in hypertensive patients. PMID: 20079160
  12. PRCP appears to be a HUVEC-associated prekallikrein activator. PMID: 11830581
  13. Identification of prolylcarboxypeptidase as the cell matrix-associated prekallikrein activator. PMID: 12123826
  14. recombinant protein, identical to enzyme from human umbilical vein endothelial cells, is a prekallikrein activator. PMID: 14996700
  15. Prolylcarboxypepdiase E112D (rs2298668)D allele alone and jointly with chronic hypertension were associated with a significantly increased risk of preeclampsia PMID: 16681991
  16. role in regulation cardiovascular tone; proinflammatory agent PMID: 18396440
  17. These investigations showed that the C-terminal region of the rPRCP(40) contributes to PRCP's catalytic function, and provided additional experimental evidence for this suggestion. PMID: 18656443

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Subcellular Location Lysosome
Protein Families Peptidase S28 family
Tissue Specificity Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
Database Links

HGNC: 9344

OMIM: 176785

KEGG: hsa:5547

STRING: 9606.ENSP00000377055

UniGene: Hs.523936

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