Recombinant Human M-phase inducer phosphatase 2 (CDC25B)

Code CSB-YP004995HU
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Source Yeast
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Code CSB-EP004995HU
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Source E.coli
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Code CSB-EP004995HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004995HU
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Source Baculovirus
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Code CSB-MP004995HU
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
AI604853 ; Cdc 25B; Cdc25b; CDC25HU2; Cdc25m2; Cell division cycle 25 homolog B; Cell division cycle 25B; Cell division cycle 25B isoform 1; Cell division cycle 25B isoform 2; Cell division cycle 25B isoform 3; Cell division cycle 25B isoform 4; Cell division cycle 25B isoform 5; Dual specificity phosphatase Cdc25B; EC; M phase inducer phosphatase 2; M-phase inducer phosphatase 2; MPIP2_HUMAN
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Protein Length
Full length protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Gene References into Functions
  1. miR-152 was a tumor suppressor in EC that inhibited proliferation of human endometrial cancer cells via inducing G2/M phase arrest by suppressing CDC25B expression. PMID: 29353204
  2. High CDC25B expression is associated with non-small cell lung cancer metastasis. PMID: 28656293
  3. YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells. PMID: 27863420
  4. While the low expression level of DUSP7 was restricted to patients with positive rheumatoid factor and anti-citrullinated protein antibodies, the altered expression of CDC25B correlated with the activity of early arthritis. PMID: 28253537
  5. Conformational flexibility of the complete catalytic domain of Cdc25B phosphatase has been demonstrated. PMID: 27410025
  6. High CDC25B expression is associated with esophageal carcinoma. PMID: 25775393
  7. Solution NMR studies reveal no global flexibility in the structure of CDC25b catalytic domain PMID: 24740794
  8. For the first time, we demonstrate that miRNA-211 is a direct negative regulator of CDC25B expression in TNBC cells, alters other related target proteins CCNB1 and FOXM1, and then inhibits breast cancer cells growth, migration, and invasion PMID: 25680404
  9. FK-3000 exerts its antiproliferative effect through G2/M cell cycle arrest via downregulation of cyclin B and phospho-CDC2 by p38 MAPK phosphorylation and CDC25B dephosphorylation . PMID: 25384584
  10. RSK promotes G2/M transition in mammalian cells through activating phosphorylation of Cdc25A and Cdc25B. PMID: 23708659
  11. Cdc25B upregulation and 14-3-3sigma downregulation might promote development of bladder cancer and suggested a poor prognosis. PMID: 24234332
  12. Positive expression of CDC25B in astrocytoma affects the prognosis in an adverse manner. PMID: 24344030
  13. CDC25B is a pro-influenza A virus host factor. PMID: 24109234
  14. CDC25B, through activation of a centrosomal pool of CDK2, stabilises the local pool of Mps1 which in turn regulates the level of centrin 2 at the centrosome. PMID: 23840880
  15. Phosphorylation and inhibition of cdc25B causes delayed progression from G2 into mitosis in melanoma cells. PMID: 23908401
  16. Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal ke PMID: 22721997
  17. downregulation of Cdc25B resulted in slower growth, more G2/M cells, weaker capacity for migration and invasion and induction of apoptosis in renal carcinoma cell line 769-P transfectants; findings suggest an important role of Cdc25B in renal cell carcinoma development PMID: 22631674
  18. Transcriptional repression mediated by IER5 regulates Cdc25B expression levels via the release of NF-YB and p300 in acute myeloid leukemia. PMID: 22132193
  19. We identified CDC25B as novel miR-148a target which may confer a proliferative advantage in pancreatic ductal adenocarcinomas. PMID: 21709669
  20. Ser169 phosphorylation can disrupt 14-3-3 binding to Ser151 activating Cdc25B3, providing a mechanism for regulating Cdc25B3 activation without dephosphorylation of the 14-3-3 binding sites. PMID: 21558810
  21. there are 13 sites phosphorylated by PLK1 in CDC25B.This study illustrates the complexity of the phosphorylation pattern and of the subsequent regulation of CDC25B activity. PMID: 21640712
  22. Deletion and mutation analyses of the Cdc25B promoter revealed that downregulation by p53 is dependent on the presence of functional Sp1/Sp3 and NF-Y binding sites PMID: 21242964
  23. CDC25B forms a close association with Ctn-2 proteins at the centrosome. PMID: 21091437
  24. Our findings reveal the existence of a previously unrecognized CDC25B isoform that operates specifically in the nucleus to reinitiate G2/M transition after DNA damage PMID: 21363925
  25. The mitotic phosphorylation of Ser(321) acts to maintain full activation of Cdc25B by disrupting 14-3-3 binding to Ser(323) and enhancing the dephosphorylation of Ser(323) to block 14-3-3 binding to this site. PMID: 20801879
  26. CDC25B a minor role in the pathogenesis and/or progression of vulvar squamous cell carcinomas. PMID: 20500813
  27. A moderate and unscheduled increase in CDC25B level, as observed in a number of human tumours, is sufficient to overcome the S-phase checkpoint efficiency thus leading to replicative stress and genomic instability. PMID: 20128929
  28. A new mechanism of CDC25B regulation in response to stress. PMID: 20176018
  29. Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B phosphatases. PMID: 20025242
  30. nuclear localization and serine 146 integrity required for induction of mitosis PMID: 12107172
  31. pEg3 kinase is able to specifically phosphorylate CDC25B in vitro. One phosphorylation site was identified and corresponded to serine 323 PMID: 12400006
  32. we demonstrate that phosphorylation of CDC25B by protein kinase CK2 increases the catalytic activity of the phosphatase in vitro as well as in vivo.CDC25B interacts with CK2, and this interaction, mediated by the CK2beta regulatory subunit PMID: 12527891
  33. upregulation of Cdc25B in human prostate cancer and its interplay with androgen receptor may contribute to prostate cancer development PMID: 12569365
  34. Data show that FHL3 (human four-and-a-half LIM-only protein 3) interacts with human phosphatase CDC25B in the cell nucleus. PMID: 12681290
  35. Cdc25B may play important roles in the development and progression of endometrioid endometrial carcinoma and clear cell carcinoma and uterine pappillary serous carcinoma by different mechanism. PMID: 14559803
  36. cdc25B plays a role in the early phase of thyroid lymphoma possibly including the malignant transformation from chronic thyroiditis PMID: 14767575
  37. Identification of a nuclear export signal in CDC25B. PMID: 15003534
  38. Phosphorylation of CDC25B by AURA at the centrosome contributes to the G2-M transition. PMID: 15128871
  39. 14-3-3 subtypes can control the subcellular localization of CDC25B by binding to a specific site. PMID: 15173315
  40. Cdc25B localisation and nuclear export changes during the cell cycle and in response to stress PMID: 15456846
  41. Results suggest that cdc25B is important especially in the early phase of breast carcinoma progression. PMID: 15550849
  42. These results support a model in which pEg3 contributes to the control of progression through mitosis by phosphorylation of the CDC25 phosphatases. PMID: 15908796
  43. Aurora-A-mediated phosphorylation of CDC25B at the centrosome is an important step contributing to the earliest events inducing mitosis, upstream of CDK1-cyclin B1 activation PMID: 16082213
  44. MAPKAP kinase-2 phosphorylates CDC25B on multiple sites including S169, S323, S353 and S375, while p38SAPK phosphorylates CDC25B on S249. PMID: 16861915
  45. Results show under normal cell cycle conditions and in the absence of DNA damage, CDC25B is constitutively phosphorylated by CHK1 during interphase and thus prevents the premature initiation of mitosis. PMID: 17003105
  46. Chk1 functions to coordinate mitotic events through regulation of Cdc25B PMID: 17106257
  47. The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells. PMID: 17336969
  48. p38 regulates the timing of mitotic entry via modulation of Cdc25B activity under normal nonstress conditions PMID: 17548358
  49. Cdc25B, but not Cdc25C, is capable of inhibiting cellular proliferation in a manner dependent upon its catalytic activity PMID: 17591782
  50. CDC25B splice variants have differential mitotic stabilities, a feature that is likely to have major consequences on the local control of cyclin-dependent kinase-cyclin activities during mitotic progression. PMID: 17599046

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Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
Protein Families
MPI phosphatase family
Database Links

HGNC: 1726

OMIM: 116949

KEGG: hsa:994

STRING: 9606.ENSP00000245960

UniGene: Hs.153752

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