Recombinant Human Matrix metalloproteinase-28 (MMP28)

Code CSB-YP880950HU
MSDS
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Source Yeast
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Code CSB-EP880950HU
MSDS
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Source E.coli
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Code CSB-EP880950HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880950HU
MSDS
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Source Baculovirus
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Code CSB-MP880950HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MMP28
Uniprot No.
Alternative Names
Epilysin; Matrix metalloproteinase 28; Matrix metalloproteinase-28; MM 28; MM28; MMP 25; MMP 28; MMP-28; MMP25; MMP28; MMP28_HUMAN
Species
Homo sapiens (Human)
Expression Region
123-520
Target Protein Sequence
FAKQGNKW YKQHLSYRLV NWPEHLPEPA VRGAVRAAFQ LWSNVSALEF WEAPATGPAD IRLTFFQGDH NDGLGNAFDG PGGALAHAFL PRRGEAHFDQ DERWSLSRRR GRNLFVVLAH EIGHTLGLTH SPAPRALMAP YYKRLGRDAL LSWDDVLAVQ SLYGKPLGGS VAVQLPGKLF TDFETWDSYS PQGRRPETQG PKYCHSSFDA ITVDRQQQLY IFKGSHFWEV AADGNVSEPR PLQERWVGLP PNIEAAAVSL NDGDFYFFKG GRCWRFRGPK PVWGLPQLCR AGGLPRHPDA ALFFPPLRRL ILFKGARYYV LARGGLQVEP YYPRSLQDWG GIPEEVSGAL PRPDGSIIFF RDDRYWRLDQ AKLQATTSGR WATELPWMGC WHANSGSALF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Can degrade casein. Could play a role in tissues homeostasis and repair.
Gene References into Functions
  1. Circulating MMP-28 was elevated in atrial fibrillation PMID: 29729262
  2. Results suggest a crucial role of MMP9 at the early stage of carcinogenesis in the large intestine. The increase in MMP9 and TIMP1 mRNA concentration and the decrease in MMP28 in the large intestinal tissue may be a confirmation of cancer. PMID: 28293015
  3. These results proved for the first time that epilysin expression was significantly elevated in glioblastoma PMID: 25429835
  4. A decreased level of IL-33 and an elevated concentration of MMP-28 were found in coronary heart disease patients and correlated with disease severity. PMID: 24710352
  5. MMP28 mRNA expression is highest in healthy tissues when compared to diseased periodontal tissues. PMID: 24167355
  6. Data established a seven-gene (AR, ESR2, GATA3, GBX2, KRT16, MMP28 and WNT11) prognostic signature to define a subset of triple-negative breast cancer (TNBC). PMID: 23549873
  7. Over-expression of MMP28 provides protection against apoptosis induced by either serum-deprivation or treatment with a protein kinase inhibitor. PMID: 22040290
  8. Gene expression of MMP28 in the intervertebral disk is not regulated by inflammatory mechanisms, is donor-dependent and cannot be positively or negatively linked to the grade of degeneration and only weakly to the occurrence of trauma. PMID: 21801383
  9. basal expression of MMP-2, MMP-9, MMP-28, and Filaggrin was evaluated in oral keratinocytes to collect information about ability of cigarette smoke to modify basal expression pattern of these key enzymes in the absence of clinical signs in oral epithelium PMID: 21723775
  10. MMP28 is frequently overexpressed during progression of gastric carcinoma, and contributes to tumor cell invasion and metastasis. PMID: 21615884
  11. Findings presented here show the first documentation of intervertebral disc expression and production of MMP28 PMID: 20003223
  12. MMP28 gene expression is regulated by Sp1 transcription factor acetylation. PMID: 20144149
  13. Expression of MMP-21 and MMP-28 seems to associate with the Merkel cell carcinoma of lesser malignant potential. PMID: 19921252
  14. Epilysin (MMP-28) expression is associated with cell proliferation during epithelial repair. PMID: 12164918
  15. Results suggest inhibition of MMP-28 may be beneficial under conditions of dysmyelination. PMID: 18778487
  16. Data demonstrate that expression of MMP28 alters keratinocyte phenotype towards a more adhesive, less migratory behaviour. PMID: 19375502
  17. Epilysin (MMP-28) is deposited to the basolateral extracellular matrix of epithelial cells. PMID: 19379669

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Tissue Specificity
Expressed at high levels in testes and lung. Low levels are detected in kidney, pancreas and skin. Also expressed in fetal lung, brain, skeletal muscle and kidney. Expressed selectively in keratinocytes. Widely expressed in several carcinomas as well. Is
Database Links

HGNC: 14366

OMIM: 608417

KEGG: hsa:79148

STRING: 9606.ENSP00000250144

UniGene: Hs.380710

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