MMP28 Antibody

Datasheet

Code	CSB-PA880950LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: MMP28 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 15 kDa Observed band size: 59 kDa IHC image of CSB-PA880950LA01HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA880950LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA880950LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MMP28 Polyclonal antibody

Uniprot No.

Q9H239

Target Names

MMP28

Alternative Names

Epilysin antibody; Matrix metalloproteinase 28 antibody; Matrix metalloproteinase-28 antibody; MM 28 antibody; MM28 antibody; MMP 25 antibody; MMP 28 antibody; MMP-28 antibody; MMP25 antibody; MMP28 antibody; MMP28_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Matrix metalloproteinase-28 protein (351-475AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MMP28 Antibody (Product code: CSB-PA880950LA01HU) is Non-conjugated. For MMP28 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA880950LB01HU	MMP28 Antibody, HRP conjugated	ELISA
FITC	CSB-PA880950LC01HU	MMP28 Antibody, FITC conjugated
Biotin	CSB-PA880950LD01HU	MMP28 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Can degrade casein. Could play a role in tissues homeostasis and repair.

Gene References into Functions

Circulating MMP-28 was elevated in atrial fibrillation PMID: 29729262
Results suggest a crucial role of MMP9 at the early stage of carcinogenesis in the large intestine. The increase in MMP9 and TIMP1 mRNA concentration and the decrease in MMP28 in the large intestinal tissue may be a confirmation of cancer. PMID: 28293015
These results proved for the first time that epilysin expression was significantly elevated in glioblastoma PMID: 25429835
A decreased level of IL-33 and an elevated concentration of MMP-28 were found in coronary heart disease patients and correlated with disease severity. PMID: 24710352
MMP28 mRNA expression is highest in healthy tissues when compared to diseased periodontal tissues. PMID: 24167355
Data established a seven-gene (AR, ESR2, GATA3, GBX2, KRT16, MMP28 and WNT11) prognostic signature to define a subset of triple-negative breast cancer (TNBC). PMID: 23549873
Over-expression of MMP28 provides protection against apoptosis induced by either serum-deprivation or treatment with a protein kinase inhibitor. PMID: 22040290
Gene expression of MMP28 in the intervertebral disk is not regulated by inflammatory mechanisms, is donor-dependent and cannot be positively or negatively linked to the grade of degeneration and only weakly to the occurrence of trauma. PMID: 21801383
basal expression of MMP-2, MMP-9, MMP-28, and Filaggrin was evaluated in oral keratinocytes to collect information about ability of cigarette smoke to modify basal expression pattern of these key enzymes in the absence of clinical signs in oral epithelium PMID: 21723775
MMP28 is frequently overexpressed during progression of gastric carcinoma, and contributes to tumor cell invasion and metastasis. PMID: 21615884
Findings presented here show the first documentation of intervertebral disc expression and production of MMP28 PMID: 20003223
MMP28 gene expression is regulated by Sp1 transcription factor acetylation. PMID: 20144149
Expression of MMP-21 and MMP-28 seems to associate with the Merkel cell carcinoma of lesser malignant potential. PMID: 19921252
Epilysin (MMP-28) expression is associated with cell proliferation during epithelial repair. PMID: 12164918
Results suggest inhibition of MMP-28 may be beneficial under conditions of dysmyelination. PMID: 18778487
Data demonstrate that expression of MMP28 alters keratinocyte phenotype towards a more adhesive, less migratory behaviour. PMID: 19375502
Epilysin (MMP-28) is deposited to the basolateral extracellular matrix of epithelial cells. PMID: 19379669

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Peptidase M10A family

Tissue Specificity

Expressed at high levels in testes and lung. Low levels are detected in kidney, pancreas and skin. Also expressed in fetal lung, brain, skeletal muscle and kidney. Expressed selectively in keratinocytes. Widely expressed in several carcinomas as well. Is

Database Links

HGNC: 14366

OMIM: 608417

KEGG: hsa:79148

STRING: 9606.ENSP00000250144

UniGene: Hs.380710

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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