Name: Recombinant Human Metallothionein-1A(MT1A),partial
Brand: CUSABIO
SKU: CSB-EP015107HU1

Product Details

Purity	Greater than 90% as determined by SDS-PAGE.
Target Names	MT1A
Uniprot No.	P04731
Research Area	Epigenetics and Nuclear Signaling
Alternative Names	CES 1; CES1; Metallothionein 1A; Metallothionein 1S; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein-1A; Metallothionein-IA; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT-1A; MT-IA; MT1; MT1A; MT1A_HUMAN; MT1S; MT2; MT2A; MTC
Species	Homo sapiens (Human)
Source	E.coli
Expression Region	1-59aa
Target Protein Sequence	MDPNCSCATGGSCTCTGSCKCKECKCTSCKKSCCSCCPMSCAKCAQGCICKGASEKCSC Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight	32.9kDa
Protein Length	Partial
Tag Info	N-terminal GST-tagged
Form	Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function (From Uniprot)	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Gene References into Functions	Full-length recombinant MT isoform 1a and it's isolated domain fragments were alkylated, changing protein conformation. PMID: 29518586 MT1A is aberrantly silenced by DNA methylation of 5' MT1A CpG island in melanoma. PMID: 28764861 Using a combination of electrospray ionization mass spectrometry (ESI-MS), circular dichroism (CD), and emission spectroscopy, this study reports that the Cu(i) to human apo-MT1A binding mechanism is highly pH-dependent. PMID: 28466911 Disruption of metallothionein I and II genes in mouse produced viable mice with increased susceptibility to cadmium poisoning. PMID: 8290567 Selective cysteine modification of metal-free human metallothionein 1a and its isolated domain fragments: Solution structural properties revealed via ESI-MS. PMID: 28187517 blockade of metallothioneins 1 and 2 constitutes a promising approach for the treatment of conditions which result in muscle atrophy. PMID: 27956698 Neonatal phthalate ester exposure induced placental MTs, FATP1 and HFABP mRNA expression PMID: 26867681 The present study was undertaken to explore further the interrelationship between p53 and metallothioneins. PMID: 27049123 MTF1 heads a hierarchy of zinc sensors, and through controlling the expression of a raft of metallothioneins and other key proteins involved in controlling intracellular zinc levels (e.g. ZnT1) alters zinc buffering capacity and total cellular zinc content. PMID: 26824222 These results clearly suggest that MT-1 may be involved in AD pathogenesis. PMID: 26836194 Zn(ii) and Cd(ii) metalation of the human MT1a takes place through two distinct pathways. PMID: 26583802 We report on the competitive zinc metalation of apo-carbonic anhydrase [CA; metal-free CA (apo-CA)] in the presence of apo-metallothionein 1A domain fragments to identify domain specific determinants of zinc binding and zinc donation PMID: 26475450 The Zinc and Cadmium exchange kinetics between human MT1A and carbonic anhydrase were examined using time-dependent electrospray ionization mass spectrometry. PMID: 26401817 Modeling of the reactions showed that at both physiological (7.4) and acidic (5.8) pHs, zinc binding and cadmium exchanges occur essentially randomly between two MT1A fragments. PMID: 26167879 Data indicate the calculated equilibrium zinc binding constants of each of the 7 zinc metallothionein 1A species ranged from a high of (log(KF)) 12.5 to a low of 11.8. PMID: 25208334 Low MT1A expression is associateed with lung carcinogenesis. PMID: 23947958 The metal-free, apo-alpha-MT also adopts a folded structure in the presence of the As(3+) even though there is no As(3+) bound. PMID: 24140052 Increased metallothionein expression reflects steroid resistance in renal allograft recipients. PMID: 23763497 Polymorphisms in the MT1A gene may influence excretion of urine uric acid and N-acetyl-beta-D-glucosamine in chronic lead-exposed workers. PMID: 23429061 MT-1A is epigenetically regulated by PU.1 during monocytic differentiation. PMID: 23501100 During the titration with Zn(2+), the electrospray ionization mass spectrometry data show that several metalated species coexist until the fully saturated proteins are formed. PMID: 23506369 MT1A rs11076161 was associated with B-Cd concentrations and Cd-induced kidney toxicity at high exposure levels. PMID: 22995156 A positive correlation between MT and Ki-67 expression was observed for all the studied cases but was even stronger in the metatypic subtype of basal cell carcinoma. PMID: 23042264 Variations in the ability of LAT1/DMT1/MTF1/MT1a to process and transport Hg may not play a significant role in the etiology of autism. PMID: 21798283 Possible role of MT as a marker of cell stress and homeostasis restoration in Graves'disease. PMID: 22090273 The expression of metallothioneins MT1A and MT1X was significantly downregulated during differentiation of Caco-2 cells treated with high levels of zinc. PMID: 21103883 MT-1A, -1F, -1G, -1X and -2A isoforms are significantly down-regulated in proliferating keloid fibroblasts. PMID: 20812968 Data show that MT-I + II and megalin are significantly altered in CNS lymphoma relative to controls. PMID: 20038220 Metallothionein, potential interaction partner for ECRG2, might be involved in regulation of cell proliferation and apoptosis and in various physiological processes. PMID: 12970870 metallothionein measured in renal specimens from cadaver kidneys was restricted to tubular cells with no differences between controls and patients with death due to chronic diseases PMID: 15812196 The overexpression of human MT1A gene dynamically affected cell viability, and the effect was influenced by zinc and cadmium ions. PMID: 16087360 MT-II mRNA expression may be involved in cell proliferation in the livers of patients with chronic HCV infection. PMID: 16107899 +647 MT1a genetic polymorphism may be essential for longevity in women. PMID: 16955215 analysis of glial fibrillary acidic protein, metallothionein, and MHC II expression in human, rat and mouse cells PMID: 17008879 The findings define a pathway for cellular metal acquisition. The results suggest a function of MT in intercellular communication. PMID: 17111383 One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is metallothionein. PMID: 17373731 The partially metallated and metal-free metallothionein-1a species are stable intermediates and thus may have a potential role in the currently undefined function of metallothionein. PMID: 17388808 The goals of this study were to define the expression of the isoforms of MT 1, 2, 3 at both mRNA and protein levels, in normal prostate, benign prostatic hyperplasia (BPH) and malignant PC-3 cells. PMID: 18208603 the association of the +647 A/C MT1A polymorphism with diabetes mellitus 2 and cardiovascular complications PMID: 18249147 the interleukin-6 and metallothionein 1a genes act in a concerted manner to control zinc-regulated gene expression PMID: 18316168 Metal exchange in metallothioneins: a novel structurally significant Cd(5) species in the alpha domain of MT1A. PMID: 18429853 This study provides the necessary data establishing unambiguously the noncooperative nature of cadmium binding to both isolated domains and the combined beta-domains (beta-rhMT) of human MT-1a. PMID: 18533113 Lupus nephritis is associated with significant alterations in renal MT-I+II expression. Important prognostic information can be deduced from the renal MT-I+II expression profile in systemic lupus erythematosus patients with nephritis. PMID: 18601746 MT-IA mRNA expression in HPBLs may be used as a biomarker for renal dysfunction in occupational cadmium exposure. PMID: 19359654 HIF-1alpha expression qualified as an independent prognostic and characterised an aggressive cancer phenotype associated with an increased expression of MT. PMID: 19529947 Show More Hide All
Protein Families	Metallothionein superfamily, Type 1 family
Database Links	HGNC: 7393 OMIM: 156350 KEGG: hsa:4489 STRING: 9606.ENSP00000290705 UniGene: Hs.655199

CloseMessage

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Code	CSB-EP015107HU1
Size	$1812Purchase it in Cusabio online store (only available for customers from the US)
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Metallothionein-1A(MT1A),partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

CloseMessage

×CloseMessage