Human Metallothionein,MT ELISA Kit

Code CSB-E09060h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
Alternative Names
CES 1 ELISA Kit; CES1 ELISA Kit; Metallothionein 1A ELISA Kit; Metallothionein 1S ELISA Kit; Metallothionein 2 ELISA Kit; Metallothionein 2A ELISA Kit; Metallothionein IA ELISA Kit; Metallothionein II ELISA Kit; Metallothionein-1A ELISA Kit; Metallothionein-IA ELISA Kit; Metallothionein2 ELISA Kit; MGC32848 ELISA Kit; MT 1A ELISA Kit; MT 2 ELISA Kit; MT 2A ELISA Kit; MT IA ELISA Kit; MT II ELISA Kit; MT-1A ELISA Kit; MT-IA ELISA Kit; MT1 ELISA Kit; MT1A ELISA Kit; MT1A_HUMAN ELISA Kit; MT1S ELISA Kit; MT2 ELISA Kit; MT2A ELISA Kit; MTC ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, urine, saliva
Detection Range
6.25 pg/mL-400 pg/mL
0.813 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle


Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.




To assess the linearity of the assay, samples were spiked with high concentrations of human MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.



Typical Data


These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MT antibody.
  • Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial HRP-conjugated MT antibody (6 ml/bottle) ---Bind to the MT in the samples or standards and react with the substrate to make the solution chromogenic.
  • Substrate A (1 x 7 ml)
  • Substrate B (1 x 7 ml)
  • One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Human Metallothionein (MT) ELISA Kit was designed for the quantitative measurement of Human Metallothionein (MT) protein in serum, plasma, cell culture supernates, tissue homogenates, urine, saliva. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 0.813 pg/mL.

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I want to know the isoforms detected in the CSB-E09060h Human metallothionein ELISA Kit (Colorimetric).

Thanks for your inquiry.
This kit is designed according to:

Target Background

(From Uniprot)
Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Gene References into Functions
  1. Full-length recombinant MT isoform 1a and it's isolated domain fragments were alkylated, changing protein conformation. PMID: 29518586
  2. MT1A is aberrantly silenced by DNA methylation of 5' MT1A CpG island in melanoma. PMID: 28764861
  3. Using a combination of electrospray ionization mass spectrometry (ESI-MS), circular dichroism (CD), and emission spectroscopy, this study reports that the Cu(i) to human apo-MT1A binding mechanism is highly pH-dependent. PMID: 28466911
  4. Disruption of metallothionein I and II genes in mouse produced viable mice with increased susceptibility to cadmium poisoning. PMID: 8290567
  5. Selective cysteine modification of metal-free human metallothionein 1a and its isolated domain fragments: Solution structural properties revealed via ESI-MS. PMID: 28187517
  6. blockade of metallothioneins 1 and 2 constitutes a promising approach for the treatment of conditions which result in muscle atrophy. PMID: 27956698
  7. Neonatal phthalate ester exposure induced placental MTs, FATP1 and HFABP mRNA expression PMID: 26867681
  8. The present study was undertaken to explore further the interrelationship between p53 and metallothioneins. PMID: 27049123
  9. MTF1 heads a hierarchy of zinc sensors, and through controlling the expression of a raft of metallothioneins and other key proteins involved in controlling intracellular zinc levels (e.g. ZnT1) alters zinc buffering capacity and total cellular zinc content. PMID: 26824222
  10. These results clearly suggest that MT-1 may be involved in AD pathogenesis. PMID: 26836194
  11. Zn(ii) and Cd(ii) metalation of the human MT1a takes place through two distinct pathways. PMID: 26583802
  12. We report on the competitive zinc metalation of apo-carbonic anhydrase [CA; metal-free CA (apo-CA)] in the presence of apo-metallothionein 1A domain fragments to identify domain specific determinants of zinc binding and zinc donation PMID: 26475450
  13. The Zinc and Cadmium exchange kinetics between human MT1A and carbonic anhydrase were examined using time-dependent electrospray ionization mass spectrometry. PMID: 26401817
  14. Modeling of the reactions showed that at both physiological (7.4) and acidic (5.8) pHs, zinc binding and cadmium exchanges occur essentially randomly between two MT1A fragments. PMID: 26167879
  15. Data indicate the calculated equilibrium zinc binding constants of each of the 7 zinc metallothionein 1A species ranged from a high of (log(KF)) 12.5 to a low of 11.8. PMID: 25208334
  16. Low MT1A expression is associateed with lung carcinogenesis. PMID: 23947958
  17. The metal-free, apo-alpha-MT also adopts a folded structure in the presence of the As(3+) even though there is no As(3+) bound. PMID: 24140052
  18. Increased metallothionein expression reflects steroid resistance in renal allograft recipients. PMID: 23763497
  19. Polymorphisms in the MT1A gene may influence excretion of urine uric acid and N-acetyl-beta-D-glucosamine in chronic lead-exposed workers. PMID: 23429061
  20. MT-1A is epigenetically regulated by PU.1 during monocytic differentiation. PMID: 23501100
  21. During the titration with Zn(2+), the electrospray ionization mass spectrometry data show that several metalated species coexist until the fully saturated proteins are formed. PMID: 23506369
  22. MT1A rs11076161 was associated with B-Cd concentrations and Cd-induced kidney toxicity at high exposure levels. PMID: 22995156
  23. A positive correlation between MT and Ki-67 expression was observed for all the studied cases but was even stronger in the metatypic subtype of basal cell carcinoma. PMID: 23042264
  24. Variations in the ability of LAT1/DMT1/MTF1/MT1a to process and transport Hg may not play a significant role in the etiology of autism. PMID: 21798283
  25. Possible role of MT as a marker of cell stress and homeostasis restoration in Graves'disease. PMID: 22090273
  26. The expression of metallothioneins MT1A and MT1X was significantly downregulated during differentiation of Caco-2 cells treated with high levels of zinc. PMID: 21103883
  27. MT-1A, -1F, -1G, -1X and -2A isoforms are significantly down-regulated in proliferating keloid fibroblasts. PMID: 20812968
  28. Data show that MT-I + II and megalin are significantly altered in CNS lymphoma relative to controls. PMID: 20038220
  29. Metallothionein, potential interaction partner for ECRG2, might be involved in regulation of cell proliferation and apoptosis and in various physiological processes. PMID: 12970870
  30. metallothionein measured in renal specimens from cadaver kidneys was restricted to tubular cells with no differences between controls and patients with death due to chronic diseases PMID: 15812196
  31. The overexpression of human MT1A gene dynamically affected cell viability, and the effect was influenced by zinc and cadmium ions. PMID: 16087360
  32. MT-II mRNA expression may be involved in cell proliferation in the livers of patients with chronic HCV infection. PMID: 16107899
  33. +647 MT1a genetic polymorphism may be essential for longevity in women. PMID: 16955215
  34. analysis of glial fibrillary acidic protein, metallothionein, and MHC II expression in human, rat and mouse cells PMID: 17008879
  35. The findings define a pathway for cellular metal acquisition. The results suggest a function of MT in intercellular communication. PMID: 17111383
  36. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is metallothionein. PMID: 17373731
  37. The partially metallated and metal-free metallothionein-1a species are stable intermediates and thus may have a potential role in the currently undefined function of metallothionein. PMID: 17388808
  38. The goals of this study were to define the expression of the isoforms of MT 1, 2, 3 at both mRNA and protein levels, in normal prostate, benign prostatic hyperplasia (BPH) and malignant PC-3 cells. PMID: 18208603
  39. the association of the +647 A/C MT1A polymorphism with diabetes mellitus 2 and cardiovascular complications PMID: 18249147
  40. the interleukin-6 and metallothionein 1a genes act in a concerted manner to control zinc-regulated gene expression PMID: 18316168
  41. Metal exchange in metallothioneins: a novel structurally significant Cd(5) species in the alpha domain of MT1A. PMID: 18429853
  42. This study provides the necessary data establishing unambiguously the noncooperative nature of cadmium binding to both isolated domains and the combined beta-domains (beta-rhMT) of human MT-1a. PMID: 18533113
  43. Lupus nephritis is associated with significant alterations in renal MT-I+II expression. Important prognostic information can be deduced from the renal MT-I+II expression profile in systemic lupus erythematosus patients with nephritis. PMID: 18601746
  44. MT-IA mRNA expression in HPBLs may be used as a biomarker for renal dysfunction in occupational cadmium exposure. PMID: 19359654
  45. HIF-1alpha expression qualified as an independent prognostic and characterised an aggressive cancer phenotype associated with an increased expression of MT. PMID: 19529947

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Protein Families
Metallothionein superfamily, Type 1 family
Database Links

HGNC: 7393

OMIM: 156350

KEGG: hsa:4489

STRING: 9606.ENSP00000290705

UniGene: Hs.655199

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