Recombinant Human Metastasis-associated protein MTA3 (MTA3)

Code CSB-YP863118HU
MSDS
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Source Yeast
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Code CSB-EP863118HU
MSDS
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Source E.coli
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Code CSB-EP863118HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863118HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP863118HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MTA3
Uniprot No.
Alternative Names
1110002J22Rik; fj99h01; KIAA1266; Metastasis associated 1 family, member 3; Metastasis associated 3; Metastasis associated family, member 3; Metastasis associated gene 3; Metastasis associated gene family, member 3; Metastasis associated protein MTA3; Metastasis-associated protein MTA3; MGC56396; MGC77410; mKIAA1266; mta1; Mta3; MTA3 metastasis associated 1 family, member 3; Mta3 metastasis associated 3; MTA3_HUMAN; wu:fj99h01; zgc:56396
Species
Homo sapiens (Human)
Expression Region
1-594
Target Protein Sequence
MAANMYRVGD YVYFENSSSN PYLIRRIEEL NKTASGNVEA KVVCFYRRRD ISNTLIMLAD KHAKEIEEES ETTVEADLTD KQKHQLKHRE LFLSRQYESL PATHIRGKCS VALLNETESV LSYLDKEDTF FYSLVYDPSL KTLLADKGEI RVGPRYQADI PEMLLEGESD EREQSKLEVK VWDPNSPLTD RQIDQFLVVA RAVGTFARAL DCSSSVRQPS LHMSAAAASR DITLFHAMDT LYRHSYDLSS AISVLVPLGG PVLCRDEMEE WSASEASLFE EALEKYGKDF NDIRQDFLPW KSLTSIIEYY YMWKTTDRYV QQKRLKAAEA ESKLKQVYIP TYSKPNPNQI STSNGKPGAV NGAVGTTFQP QNPLLGRACE SCYATQSHQW YSWGPPNMQC RLCAICWLYW KKYGGLKMPT QSEEEKLSPS PTTEDPRVRS HVSRQAMQGM PVRNTGSPKS AVKTRQAFFL HTTYFTKFAR QVCKNTLRLR QAARRPFVAI NYAAIRAEYA DRHAELSGSP LKSKSTRKPL ACIIGYLEIH PAKKPNVIRS TPSLQTPTTK RMLTTPNHTS LSILGKRNYS HHNGLDELTC CVSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. Contributes to transcriptional repression by BCL6.
Gene References into Functions
  1. Results show that metastasis associated 1 family member 3 (MTA3) level was decreased in colorectal cancer and significantly correlated with tumor cell invasion and metastasis, and that MTA3 might serve as a potential marker of tumor recurrence and prognosis of colorectal cancer. PMID: 28418887
  2. MTA3 is an oncogene of HCC, predicts poor prognosis of HCC, and may be a future marker of HCC treatment. PMID: 27992674
  3. As a master regulator, MTA3 governs the target selection for nucleosome remodeling and histone deacetylation and functions as a transcriptional repressor. MTA3 dysregulation is associated with tumor progression, invasion, and metastasis in various cancers. MTA is also a key regulator of E-cadherin expression and epithelial-to-mesenchymal transition. PMID: 28279208
  4. MTA3 depletion induced cell cycle arrest at the G1/S boundary. Western blotting analysis revealed that the knockdown of MTA3 decreased the protein levels of cyclin A, cyclin D1 and p-Rb. These results indicate that MTA3 plays an important role in NSCLC progression PMID: 23840517
  5. Our results demonstrated that MTA3 overexpression contributes to colorectal cancer carcinogenesis, progression, and chemoresistance. MTA3 could serve as a potential therapeutic target in colorectal cancer PMID: 28351306
  6. Studies indicate that metastasis associated family, member 3 protein (MTA3) is expressed in various tissues and is associated with different physiological functions, and appears to play more complicated roles in cancers. PMID: 27033852
  7. role in terminal trophoblast differentiation PMID: 26198267
  8. MTA3 suppress apoptosis of A549 an H157 cells by inhibiting BAX, PARP expression. PMID: 26483332
  9. This review focuses on the current knowledge about the function and regulation of MTA1 and MTA3 proteins in gynecological cancer, including ovarian, endometrial, and cervical tumors. PMID: 25319202
  10. MAT3 over-expression in non-small cell lung carcinoma and MAT3 mRNA level is a risk factor for lymph node metastasis and survival. PMID: 24107548
  11. Using western blotting and luciferase assays, MTA3 was identified as a target of miR-495 PMID: 24293376
  12. MTA3 expression is an independent prognostic factor in patients with gastroesophageal junction adenocarcinoma. PMID: 23671646
  13. Down-regulation of MTA3 and up-regulation of CGB5 and Snail are associated with preeclampsia. PMID: 23510993
  14. MTA3 is not a useful marker to assess and identify high-risk patients with endometrial adenocarcinomas PMID: 20865667
  15. The absence of MTA3 leads to aberrant expression of Snail, a master regulator of epithelial to mesenchymal transitions. This results in loss of expression of E-cadherin, causing changes in epithelial architecture and invasive growth. PMID: 12705869
  16. MTA3 does not repress transcription to a significant level and appears to have a diffused pattern of subcellular localization, suggesting a biological role distinct from that of the other two MTA proteins. PMID: 12920132
  17. estrogen receptor-alpha regulates metastatic tumor antigen 3 pathway PMID: 15169784
  18. Molecular dissection of the MTA3 promoter using transient transfection assays identified a composite element required for high-level transcription consisting of an SP1 site in close proximity to a consensus estrogen response element half-site. PMID: 15358836
  19. a cell type-specific subunit of the corepressor complex Mi-2/NuRD,a cofactor for BCL-6-dependent B cell fate determination. PMID: 15454082
  20. These findings identify MTA3 as an upstream physiologic repressor of Wnt4 in mammary epithelial cells. PMID: 17050676
  21. the high expression level of MTA proteins in human chorionic cells might facilitate trophoblast cell migration and neoangiogenesis PMID: 19363681

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in germinal centers of lymphoid tissues. No expression in nonepithelial cells.
Database Links

HGNC: 23784

OMIM: 609050

KEGG: hsa:57504

STRING: 9606.ENSP00000385045

UniGene: Hs.435413

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