Recombinant Human Mitochondrial import inner membrane translocase subunit TIM50 (TIMM50), partial

Code CSB-YP674736HU1
MSDS
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Source Yeast
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Code CSB-EP674736HU1
MSDS
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Source E.coli
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Code CSB-EP674736HU1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP674736HU1
MSDS
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Source Baculovirus
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Code CSB-MP674736HU1
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIMM50
Uniprot No.
Alternative Names
TIMM50; TIM50; PRO1512; Mitochondrial import inner membrane translocase subunit TIM50
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.; May participate in the release of snRNPs and SMN from the Cajal body.
Gene References into Functions
  1. Expression of TIM50 was downregulated in hypertrophic hearts. PMID: 28432072
  2. Missense mutations in TIMM50 are likely manifesting by severe intellectual disability and epilepsy accompanied by 3-methylglutaconic aciduria and variable mitochondrial complex V deficiency. PMID: 27573165
  3. TIM50 has a role in regulating cell proliferation and apoptosis through decreasing mitochondrial membrane potential in breast cancer cell PMID: 26289846
  4. the interaction of divalent metal ions with the intermembrane space domain of Tim50 (Tim50(IMS)) and the interaction of presequence peptides with Tim50(IMS) in presence of Ca(2+) ion was investigated. PMID: 23098911
  5. The far-UV CD spectra of Tim50(IMS) in native and denatured states revealed that the protein has a significantly folded secondary structure consisted of alpha-helixes and beta-sheets. PMID: 21742040
  6. Tim50 regulates 3betaHSD2 expression and activity, representing a new role for translocases in steroidogenesis. PMID: 21930695
  7. TIM50, was upregulated in a non-small cell lung carcinoma cell line (H1299) that expressed the p53 mutants R175H and R273H compared to cells lacking p53. PMID: 21621504
  8. Tim50 is important for both mitochondrial function and early neuronal development PMID: 15044455
  9. Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with coilin, snRNPs, and SMN PMID: 16008839

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Subcellular Location
Mitochondrion inner membrane; Single-pass membrane protein.; [Isoform 2]: Nucleus speckle.
Protein Families
TIM50 family
Tissue Specificity
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
Database Links

HGNC: 23656

OMIM: 607381

KEGG: hsa:92609

STRING: 9606.ENSP00000318115

UniGene: Hs.590956

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