Recombinant Human Mothers against decapentaplegic homolog 7(SMAD7)

Code CSB-YP021793HU
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Source Yeast
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Code CSB-EP021793HU
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Source E.coli
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Code CSB-EP021793HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021793HU
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Source Baculovirus
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Code CSB-MP021793HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names SMAD7
Uniprot No. O15105
Alternative Names CRCS3; FLJ16482; hSMAD 7; hSMAD7; MAD (mothers against decapentaplegic Drosophila) homolog 7; MAD; Mad homolog 7; MAD homolog 8; MAD mothers against decapentaplegic homolog 7; MADH 7; MADH 8; MADH6; MADH7; MADH8; Mothers Against Decapentaplegic Drosophila Homolog of 6; Mothers Against Decapentaplegic Drosophila Homolog of 7; Mothers against decapentaplegic homolog 7; Mothers against decapentaplegic homolog 8; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMA- AND MAD-RELATED PROTEIN 7; SMAD 7; SMAD; SMAD family member 7; SMAD, mothers against DPP homolog 7 (Drosophila); SMAD, mothers against DPP homolog 7; SMAD6; Smad7; SMAD7_HUMAN
Species Homo sapiens (Human)
Expression Region 1-426
Target Protein Sequence MFRTKRSALV RRLWRSRAPG GEDEEEGAGG GGGGGELRGE GATDSRAHGA GGGGPGRAGC CLGKAVRGAK GHHHPHPPAA GAGAAGGAEA DLKALTHSVL KKLKERQLEL LLQAVESRGG TRTACLLLPG RLDCRLGPGA PAGAQPAQPP SSYSLPLLLC KVFRWPDLRH SSEVKRLCCC ESYGKINPEL VCCNPHHLSR LCELESPPPP YSRYPMDFLK PTADCPDAVP SSAETGGTNY LAPGGLSDSQ LLLEPGDRSH WCVVAYWEEK TRVGRLYCVQ EPSLDIFYDL PQGNGFCLGQ LNSDNKSQLV QKVRSKIGCG IQLTREVDGV WVYNRSSYPI FIKSATLDNP DSRTLLVHKV FPGFSIKAFD YEKAYSLQRP NDHEFMQQPW TGFTVQISFV KGWGQCYTRQ FISSCPCWLE VIFNSR
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Gene References into Functions
  1. Expression of Smad7 was negatively regulated by miR-17-5p; Smad7 expression inactivated NF-kappaB and Wnt/beta catenin pathways. PMID: 29433423
  2. SMAD7 Polymorphisms Are Associated with Colorectal Cancer in Patients with Lynch Syndrome. PMID: 30275229
  3. OTU deubiquitinase 1 (OTUD1) directly deubiquitinates Smad7 Protein (SMAD7) and prevents its degradation. PMID: 29235476
  4. Periostin contributes to skin fibrosis by enhancing TGF-beta signaling via Smad 7 inhibition, which may lead to extracellular matrix deposition and periostin generation. PMID: 29433908
  5. HO-1 plays a key role in protecting tumor cells from apoptosis, in a process that involves Smad7 and HDAC4/5 in apoptosis of B-ALL cells PMID: 29886060
  6. Mechanistic studies reveal that TGFbeta activates the expression of microRNA-182 (miR-182), which suppresses SMAD7 protein. miR-182 silencing leads to SMAD7 upregulation on TGFbeta treatment and prevents TGFbeta-induced epithelial-mesenchymal transition and invasion of cancer cells. PMID: 27996004
  7. This study reports a proinflammatory role for SMAD7 in human gestational tissues, with SMAD7 silencing attenuating the inflammatory response. PMID: 29044425
  8. Smad7 enables STAT3 activation and promotes pluripotency independent of TGF-beta signaling. PMID: 28874583
  9. Studied expression of microRNA-375 and its target gene SMAD-7 polymorphisms (rs4939827) in colorectal cancer (CRC) patients in comparison to control subjects. A very significant correlation was found between miRNA-375 level and mutant and heterozygotes genotypes of SMAD-7 rs4939827 polymorphism in CRC. PMID: 28374902
  10. Studies indicate that the T allele of SMAD7 protein (SMAD7)single nucleotide polymorphism rs4939827 to be significantly related with an increase colorectal cancer (CRC) risk in Chinese population [Meta-analysis]. PMID: 28467803
  11. this large-scale meta-analysis indicated that SMAD7 polymorphisms (rs4464148, rs4939827, and rs12953717) correlate with CRC. PMID: 28070019
  12. Collectively, these findings demonstrate that the miR-15a/Smad-7/TGF-beta pathway is important in HBV-associated liver cancer. PMID: 28498453
  13. SMAD7 promoter is hyper-methylated both in human atherosclerotic plaques and atherosclerosis patients, which is positively associated with homocysteine levels and carotid plaque scores. PMID: 29366786
  14. Furthermore, MERS coronavirus induced apoptosis through upregulation of Smad7 and fibroblast growth factor 2 (FGF2) expression in both kidney and lung cells. PMID: 27572168
  15. findings demonstrate TGF-beta enhances expression of USP26 and reinforces SMAD7 stability by limiting the ubiquitin-mediated turnover of SMAD7; clinically, loss of USP26 correlates with high TGF-beta activity and confers poor prognosis in glioblastoma; data identify USP26 as a novel negative regulator of the TGF-beta pathway and suggest that loss of USP26 expression may be an important factor in glioblastoma pathogenesis PMID: 28381482
  16. In conclusion, our study demonstrated that COL1A2-AS1/miR-21/Smad pathway plays an important role in inhibiting hypertrophic scar formation, and suggested this novel pathway may be a new target for hypertrophic scar treatment. PMID: 29117538
  17. Our data revealed the SMAD7 loci is associated with hepatocellular carcinoma susceptibility and its clinicopathologic development. PMID: 26989026
  18. Smad7 expression in necrotizing enterocolitis macrophages interrupts TGF-beta signaling and promotes NF-kappaB-mediated inflammatory signaling in these cells through increased expression of IKK-beta PMID: 26859364
  19. SMAD7 gene and specifically the allele C could be protective for colorectal cancer (CRC). An independent protective association was also observed between high adherence Mediterranean diet pattern and CRC risk. PMID: 29084532
  20. Smad7 inhibition diminishes interaction of PKR with protein kinase inhibitor p58 (p58(IPK)). PMID: 28300830
  21. Smad7 regulates TGF-beta1 signaling via a negative feedback loop and mediates the interaction between TGF-beta1 and other signaling pathways; suggesting that Smad7 over expression may have therapeutic potential in ALL. PMID: 28732737
  22. Missense variant in smad7 gene is associated with colorectal cancer. PMID: 28218435
  23. ASPP2 suppresses invasion, peritoneal dissemination and TGF-beta1-induced EMT by inhibiting Smad7 degradation mediated by ITCH in gastric cancer cells. PMID: 28400336
  24. Transforming Growth Factor beta inhibitor peptide P144 downregulates SKI and an upregulates SMAD7 at both transcriptional and translational levels in glioblastoma cell lines. PMID: 27473823
  25. The inverse correlation between Smad7 and AhR expression helps to propagate inflammatory signals in the gut in Crohn's disease. PMID: 26818761
  26. RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor beta signaling PMID: 28292929
  27. NR2F2 could promote TGF-beta-induced epithelial-mesenchymal transition of colorectal carcinoma cells and inhibit Smad7 expression via transactivation of miR-21. PMID: 28192117
  28. this study shows that in refractory celiac disease, high Smad7 associates with defective TGF-beta1 signaling and sustains inflammatory cytokine production PMID: 27861825
  29. miR-590-5p promotes osteoblast differentiation by indirectly protecting and stabilizing the Runx2 protein by targeting Smad7 gene expression. PMID: 27192628
  30. miRNA-181a appears to play a potential role in pancreatic beta-cells dysfunction via SMAD7. PMID: 26892629
  31. Carboplatin may upregulate SMAD7 through suppression of miR-21 to inhibit TGFbeta receptor signaling mediated non-small cell lung cancer cell invasion. PMID: 27185036
  32. Downregulation of Smad 7 promotes migration and invasion through enhancing epithelial mesenchymal transition in esophageal squamous cell carcinoma. PMID: 27619676
  33. miR-424-5p -SMAD7 pathway contributed to esophageal squamous cell carcinoma invasion and metastasis. PMID: 27628042
  34. Polymorphisms in the SMAD7 rs4939827 is associated with rectal cancer. PMID: 26779637
  35. microRNA-497 modulates breast cancer cell proliferation, invasion, and survival by targeting SMAD7. PMID: 27303812
  36. SMAD7 overexpression might represent a mechanism limiting TGF-beta-mediated fibrogenesis in human hepatic diseases; therefore, SMAD7 induction likely represents a candidate for novel therapeutic approaches. PMID: 26856334
  37. Data show that protein kinase LKB1 physically interacts with BMP type I receptors and requires Smad7 protein to promote downregulation of the receptor. PMID: 26701726
  38. Study founds the levels of CYLD and SMAD7 significantly decreased in oral squamous cell carcinoma (OSSC) cells and proposes a model that CYLD suppresses OSCC metastases through SMAD7. Downregulation of CYLD appears to directly contribute to the distal metastases of primary OSCC and subsequently poor prognosis. PMID: 26982322
  39. Data indicate that, in irritable bowel disease, high Smad7 contributes to sustain detrimental immune responses and knockdown of this molecule can help attenuate the ongoing mucosal inflammation in patients with such disorders PMID: 26651974
  40. Results showed that SMAD7 was up-regulated in hepatocellular carcinoma tissues and was found to be the target of UPF1. PMID: 26759305
  41. The expression of Smad7 increased. PMID: 26278416
  42. MiR-185-3p and miR-324-3p can modulate NPC cell growth and apoptosis, at least partly through targeting SMAD7. PMID: 26390174
  43. Meta-analysis showed that for SMAD7 gene, rs4939827 and rs4464148 are risk factors for colorectal cancer (CRC) among Caucasians whereas rs12953717 could elevate the susceptibility to CRC in both Caucasians and Asians. PMID: 26579801
  44. The decrease in miR-195 led to an increase in Smad7 expression and corresponding up-regulation of p65 and the AP-1 (activator protein 1) pathway, which might explain the mechanism of steroid resistance in ulcerative colitis patients PMID: 26303523
  45. Increased miR-16 expression promotes liver fibrosis through downregulation of HGF and Smad7 in hepatitis C patients. PMID: 26071245
  46. miR-181a promotes TGF-beta-mediated epithelial-to-mesenchymal transition via repression of Smad7. PMID: 24394555
  47. Results suggest that Itch is a positive regulator of the TGF-beta-mediated Smad signaling pathway via Smad7 ubiquitination and protein degradation. PMID: 25518932
  48. Overexpression of Smad7 in human HaCaT keratinocyte cells and mouse skin tissues elevated EGF receptor (EGFR) activity by impairing ligand-induced ubiquitination and degradation of activated receptor, which is induced by the E3 ubiquitin ligase c-Cbl. PMID: 26055326
  49. The study found a significant association between colorectal cancer risk and rs4464148 AG genotype of the SMAD7 gene in affected women, but did not detect the same association in colorectal cancer male patients. PMID: 25640388
  50. SMAD7 is a target of miR-132 in glioma cells. PMID: 25983322

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Involvement in disease Colorectal cancer 3 (CRCS3)
Subcellular Location Nucleus. Cytoplasm.
Protein Families Dwarfin/SMAD family
Tissue Specificity Ubiquitous with higher expression in the lung and vascular endothelium.
Database Links

HGNC: 6773

OMIM: 602932

KEGG: hsa:4092

STRING: 9606.ENSP00000262158

UniGene: Hs.465087

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