Recombinant Human Myc box-dependent-interacting protein 1(BIN1)

Code CSB-YP002700HU
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Source Yeast
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Code CSB-EP002700HU
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Source E.coli
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Code CSB-EP002700HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002700HU
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Source Baculovirus
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Code CSB-MP002700HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names BIN1
Uniprot No. O00499
Alternative Names AMPH 2; AMPH2; Amphiphysin 2; Amphiphysin II; Amphiphysin like protein; amphiphysin-like; Amphiphysin-like protein; AMPHL; Bin1; BIN1_HUMAN; Box Dependant MYC Interacting Protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; DKFZp547F068; MGC10367; MGC105358; Myc box dependent interacting protein 1; Myc box-dependent-interacting protein 1; SH3P9
Species Homo sapiens (Human)
Expression Region 2-593
Target Protein Sequence AEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN FNKQLTEGTR LQKDLRTYLA SVKAMHEASK KLNECLQEVY EPDWPGRDEA NKIAENNDLL WMDYHQKLVD QALLTMDTYL GQFPDIKSRI AKRGRKLVDY DSARHHYESL QTAKKKDEAK IAKPVSLLEK AAPQWCQGKL QAHLVAQTNL LRNQAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN TFQSIAGLEE NFHKEMSKLN QNLNDVLVGL EKQHGSNTFT VKAQPSDNAP AKGNKSPSPP DGSPAATPEI RVNHEPEPAG GATPGATLPK SPSQLRKGPP VPPPPKHTPS KEVKQEQILS LFEDTFVPEI SVTTPSQFEA PGPFSEQASL LDLDFDPLPP VTSPVKAPTP SGQSIPWDLW EPTESPAGSL PSGEPSAAEG TFAVSWPSQT AEPGPAQPAE ASEVAGGTQP AAGAQEPGET AASEAASSSL PAVVVETFPA TVNGTVEGGS GAGRLDLPPG FMFKVQAQHD YTATDTDELQ LKAGDVVLVI PFQNPEEQDE GWLMGVKESD WNQHKELEKC RGVFPENFTE RVP
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Is a negative regulator of endocytosis. Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production. In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates. May be involved in the regulation of MYC activity and the control cell proliferation. Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro.
Gene References into Functions
  1. Meta-analysis validated the association of late onset Alzheimer disease with BIN1 (rs744373) variants. PMID: 29504051
  2. The findings reveal the ability of Bin1 to modify actin dynamics and provide a possible mechanistic connection between Bin1 and tau-induced pathobiological changes of the actin cytoskeleton. PMID: 28893863
  3. the association between the rs744373 polymorphism of BIN1 protein and late-onset Alzheimer's disease in East Asian, American, and European populations (Meta-Analysis) PMID: 26846281
  4. Italian family with centronuclear myopathy, carrying a novel pathogenic mutation of BIN1 gene in heterozygous state, consistent with autosomal dominant inheritance. PMID: 27854204
  5. bridging integrator 1 Gene rs7561528 polymorphism contributes to Alzheimer's disease susceptibility in East Asian and Caucasian populations PMID: 28302384
  6. Findings indicated that BIN1 restoration in NSCLC could reverse PD-L1-mediated immune escape by inactivating the c-MYC and EGFR/mitogen-activated protein kinase pathways. PMID: 28714960
  7. propose that efforts to define how genetic variants in BIN1 elevate the risk for Alzheimer's disease would behoove to consider BIN1 function in the context of its main expression in mature oligodendrocytes PMID: 27538496
  8. The results emphasize an additional level of complexity in the regulation of the interaction between BIN1 and Tau dependent on the BIN1 isoforms. PMID: 28755476
  9. Data (including data from studies using transgenic mice) suggest that the process leading to microparticle release from cardiac myocytes involves recruitment of CHMP4B protein to the forming microparticle membrane which also contains cBIN1; plasma cBIN1 is reduced in patients with heart failure as compared to control subjects. (CHMP4B = charged multivesicular body protein 4B; cBIN1 = cardiac bridging integrator 1) PMID: 28806752
  10. Low Bin1 expression is associated with esophageal squamous cell carcinoma. PMID: 28152502
  11. the depletion of BIN1 increases cellular BACE1 levels through impaired endosomal trafficking and reduces BACE1 lysosomal degradation, resulting in increased Ab production. Our findings provide a mechanistic role of BIN1 in the pathogenesis of Alzheimer disease (AD), as a novel genetic regulator of BACE1 levels and Ab production PMID: 27179792
  12. BIN1 protein expression in cerebral cortex was related to disease progression in Alzheimer's Disease patients. PMID: 27346750
  13. data show that the previously described consensus sequence PXRPXR for amphiphysin SH3 ligands is inaccurate and instead define it as an extended Class II binding motif PXXPXRpXR, where additional positive charges between the two constant arginine residues can give rise to extraordinary high SH3 binding affinity. PMID: 27268056
  14. findings support a contribution of BIN1 to individual differences in episodic memory performance among Type 2 Diabetes patients. PMID: 26947052
  15. analysis of a novel deregulated mechanism in chronic myeloid leukemia patients, indicating BIN1 and RIN1 as players in the maintenance of the abnormal RTK signaling in this hematological disease PMID: 26194865
  16. BIN1 is involved with late onset Alzheimer's disease. [review] PMID: 27773727
  17. This study supported that BIN1 contributes to the risk of Alzheimer's disease by altering neural degeneration (abnormal tau, brain atrophy and glucose metabolism) but not Abeta pathology PMID: 27003210
  18. Alterations in the BIN1 locus, previously associated with Alzheimer disease, may modify the age of onset of GBA-associated Parkinson. PMID: 26233692
  19. no association was found for either polymorphism, suggesting that these genes are not implicated in the aetiology of Alzheimer's disease in all populations. PMID: 26733302
  20. Data demonstrate that EHBP1L1 links Rab8 and the Bin1-dynamin complex, which generates membrane curvature and excises the vesicle at the endocytic recycling compartment for apical transport. PMID: 26833786
  21. In vitro studies in human Caco-2 cells showed that Bin1 antibody altered the expression of tight junction proteins and improved barrier function. PMID: 26195312
  22. This study demonistrated that BIN1 mutation releated to Centronuclear myopathy. PMID: 25957634
  23. Results show low expression of Bin1, along with high expression of IDO, are predictor for poor prognosis in esophageal squamous cell cancer and thereby could be used to establish new therapeutic strategies. PMID: 25683635
  24. The frequencies of BIN1 alleles were similar in both control and Alzheimer patients showing o no association. PMID: 26738348
  25. This study findings demonstrate that rs744373 itself or a variation in linkage disequilibrium may provide a neurogenetic mechanism for BIN.1 PMID: 25630570
  26. The study is the first to confirm the association of the variant rs7561528 adjacent to Bin1 with Sporadic Alzheimer's Disease in a Han Chinese Population. PMID: 25461955
  27. These findings suggest that an intracellular form CLU and BIN1 interaction might impact Tau function in neurons and uncover potential new mechanisms underlying the etiology of Tau pathology in in Alzheimer's disease. PMID: 25051234
  28. Reduced BIN1 expression is associated with cutaneous T-cell lymphoma. PMID: 25578476
  29. BIN1 rs744373 polymorphism is significantly associated with late onset Alzheimer disease. PMID: 25022885
  30. BIN1/M-Amphiphysin2 has a role in inducing clustering of phosphoinositides to recruit its downstream partner dynamin PMID: 25487648
  31. The release of BIN1 from hypo-poly(ADP-ribosyl)ated E2F1 is a mechanism by which serum starvation promotes E2F1-induced apoptosis. PMID: 25257171
  32. Results suggest that BIN1 is likely involved in Alzheimer's disease as a modulator of neurofibrillary tangle pathology, and that this role may extend to other human diseases that feature tau pathology PMID: 25024306
  33. These results support a role for N-WASP in amphiphysin-2-dependent nuclear positioning and triad organization and in centronuclear myopathy and myotonic dystrophy pathophysiology. PMID: 25262827
  34. specific amphiphysin 2 mutations can cause either recessive or dominant centronuclear myopathy and that both disorders involve different pathomechanisms PMID: 25260562
  35. Brain DNA methylation in BIN1 was associated with pathological Alzheimer disease. PMID: 25365775
  36. physical activity attenuated the effects of genetic risk (ie. the constellation of PICALM, BIN1, and CLU polymorphisms) on episodic memory PMID: 24660791
  37. The study compares the binding dynamics and affinities of the relevant regions for binding of c-Myc and NS5A to Bin1 SH3. The result gives further insights into the potential role of NS5A in Bin1-mediated apoptosis. PMID: 24616074
  38. These results suggest an autoinhibition model for BIN1 that involves a synergistic regulation by membrane composition and protein-protein interactions. PMID: 25350771
  39. Two mutants of BIN1 showed impaired membrane tubulation both in vivo and in vitro, and displayed characteristically different behaviors. PMID: 24755653
  40. BIN1 downregulation is linked to cancer progression and also correlates with ventricular cardiomyopathy and arrhythmia preceding heart failure. Increased BIN1 expression is linked to increased susceptibility for late-onset Alzheimer's disease. [review] PMID: 24590001
  41. Our analyses suggest that these DNA methylation changes may have a role in the onset of Alzheimer disease given that we observed them in presymptomatic subjects and that six of the validated genes connect to a known susceptibility gene network. PMID: 25129075
  42. It is the most important genetic susceptibility locus in late-onset Alzheimer's disease. PMID: 24582639
  43. BIN1 expression is increased in Alzheimer Disease brains when compared with controls. PMID: 23399914
  44. BIN1 is decreased in sporadic but not familial Alzheimer's disease or in aging PMID: 24205320
  45. REVIEW--BIN1: form, function, and Alzheimer's disease PMID: 23871436
  46. The resukts of this study highlight the possible use of plasma BIN1 as a biomarker for AD diagnosis. PMID: 23803295
  47. To our knowledge, this is the first study to show significant association between rs744373 polymorphism and AD in East Asian population. PMID: 23570733
  48. Our data demonstrate that the alteration of the muscle-specific function of BIN1 is a common pathomechanism for centronuclear myopathy, myotonic dystrophy, and IMGD. PMID: 23754947
  49. We identified rare small events overlapping CR1 and BIN1 in Alzheimer's disease and normal controls with opposite copy number variation dosage. PMID: 23202439
  50. The re-expression of BIN1 specifically compromises the proliferation of SNF5-deficient rhabdoid tumors cell lines. PMID: 22544318

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Involvement in disease Myopathy, centronuclear, 2 (CNM2)
Subcellular Location [Isoform BIN1]: Nucleus. Cytoplasm. Endosome. Cell membrane, sarcolemma, T-tubule.; [Isoform IIA]: Cytoplasm.
Tissue Specificity Ubiquitous. Highest expression in the brain and muscle. Expressed in oligodendrocytes. Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter. Isoform BIN1 is widely expressed with highest expressi
Database Links

HGNC: 1052

OMIM: 255200

KEGG: hsa:274

STRING: 9606.ENSP00000316779

UniGene: Hs.193163

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