Recombinant Human Myosin-6(MYH6),partial

Code CSB-YP015299HU
Size US$2010
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names MYH6
Uniprot No. P13533
Research Area Signal Transduction
Alternative Names Myosin heavy chain 6Myosin heavy chain, cardiac muscle alpha isoform ;MyHC-alpha
Species Homo sapiens (Human)
Source Yeast
Expression Region 160-816aa
Target Protein Sequence NAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 76.8kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

I am contacting you regarding the protein CSB-YP015299HU. I recently ordered this protein. I just realized that this protein is tagged and in 50% glycerol. I am told that glycerol can interfere in the analysis that I want to develop. Would you be able to have this protein in lyophilized form and / or without tag?​

A:
Thanks for your email.
1. CSB-YP015299HU is with N-terminal 6xHis-tagged, which is a small tag. Generally, small tags have little effect on experiment.
If the customer is worried, we can also re-express other vectors trying to remove the tag, but we can't guarantee 100% success. Based on our annual analysis data of tag removal, the success rate reaches 75%-86%.
If we succed in removing the tag, we will charge you for protein production cost + tag removal cost.
If we fail in removing the tag, we will send you the tagged protein and will only charge you for protein production cost.
Lead Time for Tag Removal: 3 working days
2. We could deliver this Tag-Free protein in lyophilized form.
Lyophilization Cost: Free of charge
Lead Time for Lyophilization: 3 working days
3. The details of the Tag-Free protein are as below.
Code: CSB-YP015299HUe1
Name: Recombinant Human Myosin-6(MYH6),partial
Expression Region: 160-816aa; Partial
Tag Info: Tag-Free
Target Protein Sequence:

NAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVI


Note: The above is only the target protein AA sequence. The complete sequence (tag sequence + target protein sequence + any possible linker sequence) will be shown in our Datasheet&COA upon shipment.
Product Type: Semi-customized Recombinant Protein
Delivery Form: Lyophilized Form
Lead Time: 41-51 working days (Lead Time for Fusion Protein Production 35-45 working days + Lead Time for Tag Removal Service 3 working days + Lead Time for Lyophilization 3 working days).
Q:

Can you provide the untagged and freeze-dried protein?
As well we ordered a protein in June. Batch #03806. Would you have an unconjugated antibody to detect this protein?

A:
Thanks for your inquiry.
Code: CSB-YP015299HUe1
Name: Recombinant Human Myosin-6(MYH6),partial
Expression Region: 160-816aa; Partial
Tag Info: Tag-Free
Target Protein Sequence:

NAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVI


Note: The above is only the target protein AA sequence. The complete sequence (tag sequence + target protein sequence + any possible linker sequence) will be shown in our Datasheet&COA upon shipment.
Product Type: Semi-customized Recombinant Protein
Delivery Form: Lyophilized Form
Lead Time: 41-51 working days (Lead Time for Fusion Protein Production 35-45 working days + Lead Time for Tag Removal Service 3 working days + Lead Time for Lyophilization 3 working days).
The following unconjugated antibody can be used to detect the above protein. The product details are confirmed as below.
Code: CSB-PA878928
Name: MYH6 Antibody
Lead Time: 2-3 working days
 Customer Reviews

Target Data

Function Muscle contraction.
Involvement in disease Atrial septal defect 3 (ASD3); Cardiomyopathy, familial hypertrophic 14 (CMH14); Cardiomyopathy, dilated 1EE (CMD1EE); Sick sinus syndrome 3 (SSS3)
Subcellular Location Cytoplasm, myofibril
Protein Families TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Database Links

HGNC: 7576

OMIM: 160710

KEGG: hsa:4624

STRING: 9606.ENSP00000348634

UniGene: Hs.278432

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