Recombinant Human Myosin-6(MYH6),partial

Code CSB-YP015299HU
Size US$1298
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names MYH6
Uniprot No. P13533
Research Area Signal Transduction
Alternative Names cardiac muscle alpha isoform; MYH6; MYH6_HUMAN; MYHC; MyHC-alpha; MyHC-beta ; MYHCA; MYHCB; Myosin heavy chain 6; Myosin heavy chain; Myosin heavy chain cardiac muscle alpha isoform; Myosin heavy chain cardiac muscle beta isoform; Myosin-6
Species Homo sapiens (Human)
Source Yeast
Expression Region 160-816aa
Target Protein Sequence NAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 76.8kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Q&A and Customer Reviews

 Q&A
Q:

I am contacting you regarding the protein CSB-YP015299HU. I recently ordered this protein. I just realized that this protein is tagged and in 50% glycerol. I am told that glycerol can interfere in the analysis that I want to develop. Would you be able to have this protein in lyophilized form and / or without tag?​

A:
Thanks for your email.
1. CSB-YP015299HU is with N-terminal 6xHis-tagged, which is a small tag. Generally, small tags have little effect on experiment.
If the customer is worried, we can also re-express other vectors trying to remove the tag, but we can't guarantee 100% success. Based on our annual analysis data of tag removal, the success rate reaches 75%-86%.
If we succed in removing the tag, we will charge you for protein production cost + tag removal cost.
If we fail in removing the tag, we will send you the tagged protein and will only charge you for protein production cost.
Lead Time for Tag Removal: 3 working days
2. We could deliver this Tag-Free protein in lyophilized form.
Lyophilization Cost: Free of charge
Lead Time for Lyophilization: 3 working days
3. The details of the Tag-Free protein are as below.
Code: CSB-YP015299HUe1
Name: Recombinant Human Myosin-6(MYH6),partial
Expression Region: 160-816aa; Partial
Tag Info: Tag-Free
Target Protein Sequence:

NAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVI


Note: The above is only the target protein AA sequence. The complete sequence (tag sequence + target protein sequence + any possible linker sequence) will be shown in our Datasheet&COA upon shipment.
Product Type: Semi-customized Recombinant Protein
Delivery Form: Lyophilized Form
Lead Time: 41-51 working days (Lead Time for Fusion Protein Production 35-45 working days + Lead Time for Tag Removal Service 3 working days + Lead Time for Lyophilization 3 working days).
Q:

Can you provide the untagged and freeze-dried protein?
As well we ordered a protein in June. Batch #03806. Would you have an unconjugated antibody to detect this protein?

A:
Thanks for your inquiry.
Code: CSB-YP015299HUe1
Name: Recombinant Human Myosin-6(MYH6),partial
Expression Region: 160-816aa; Partial
Tag Info: Tag-Free
Target Protein Sequence:

NAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVI


Note: The above is only the target protein AA sequence. The complete sequence (tag sequence + target protein sequence + any possible linker sequence) will be shown in our Datasheet&COA upon shipment.
Product Type: Semi-customized Recombinant Protein
Delivery Form: Lyophilized Form
Lead Time: 41-51 working days (Lead Time for Fusion Protein Production 35-45 working days + Lead Time for Tag Removal Service 3 working days + Lead Time for Lyophilization 3 working days).
The following unconjugated antibody can be used to detect the above protein. The product details are confirmed as below.
Code: CSB-PA878928
Name: MYH6 Antibody
Lead Time: 2-3 working days
 Customer Reviews

Target Data

Function Muscle contraction.
Gene References into Functions
  1. Three loci with high mutation frequencies, the 138665410 FOXL2 gene variant, the 23862952 MYH6 gene variant, and the 71098693 HYDIN gene variant were found to be significantly associated with sporadic Atrial Septal Defect (P<0.05); variants in FOXL2 and MYH6 were found in patients with isolated, sporadic Atrial Septal Defect (P<5x10-4). PMID: 29505555
  2. We developed an human cardiac alpha-myosin -induced myocarditis model in human HLA-DR4 transgenic mice that lack all mouse MHCII genes. PMID: 28431892
  3. The etiology of MYH6-associated HLHS can be informed using iPSCs. PMID: 27789736
  4. Rare inherited and de novo variants in 2,871 congenital heart disease probands identified GDF1, MYH6, and FLT4 as causative genes. PMID: 28991257
  5. the P830L and A1004S alphaMHC mutations alter myocyte contractility in completely different ways while at the same preserving peak intracellular calcium PMID: 28088328
  6. Data show that compound heterozygosity for recessive myosin heavy chain 6 (MYH6) mutations in patients with hypoplastic left heart and reduced systemic right ventricular ejection fraction. PMID: 26085007
  7. human alpha- and beta-cardiac myosin, as well as the mutants, show opposite mechanical and enzymatic phenotypes with respect to each other. PMID: 25937279
  8. The novel MYH6 mutation delE933 causes both structural damage of the sarcomere and functional impairments on atrial action propagation. PMID: 25717017
  9. Data from molecular dynamic/docking simulations suggest that actin-myosin binding free energy accepts contributions from both electrostatic and nonpolar forces; studies compare cardiac alpha-myosin, beta-myosin, and fast skeletal muscle myosin. PMID: 24224850
  10. R1165C mutation in MYH9 gene is associated with macroscopic hematuria and presenile cataract. PMID: 22627578
  11. Perturbations in the MYH6 head domain seem to play a major role in the genetic origin of familial Secundum-type atrial septal defects. PMID: 22194935
  12. the alpha-isoform of myosin heavy chain is the pathogenic autoantigen for CD4+ T cells in myocarditis PMID: 21436590
  13. the lifetime risk of being diagnosed with sick sinus syndrome is around 6% for non-carriers of c.2161C>T but is approximately 50% for carriers of the c.2161C>T variant PMID: 21378987
  14. data indicate that functional variants of MYH6 are associated with cardiac malformations in addition to atrial septal defect and provide a novel potential mechanism PMID: 20656787
  15. The mutations in MYH6 cause when a genetic cause can be identified, which has estimated to occur in 65% of hypertrophic cardiomyopathy. PMID: 20215591
  16. Our data provide evidence for a novel form of calcium-independent positive inotropy in failing cardiac myocytes by fast alpha-myosin motor protein gene transfer. PMID: 19801488
  17. results show that PUR proteins are capable of binding to alpha-MHC mRNA and attenuate its translational efficiency; also show robust expression of PUR proteins in failing hearts where alpha-MHC mRNA levels are suppressed PMID: 12933792
  18. Mutation in myosin heavy chain 6 causes atrial septal defect PMID: 15735645
  19. Three heterozygous MYH6 missense mutations were identified in dilated cardiomyopathy probands (P830L, A1004S, and E1457K; 4.3% of probands). A Q1065H mutation was detected in 1 of 21 hypertrophic cardiomyopathy probands. PMID: 15998695
  20. the large step of dimeric myosin VI is primarily made possible by a medial tail in each monomer that forms a rare single alpha-helix of approximately 10 nm, which is anchored to the calmodulin-bound IQ domain by a globular proximal tail. PMID: 18511944

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Involvement in disease Atrial septal defect 3 (ASD3); Cardiomyopathy, familial hypertrophic 14 (CMH14); Cardiomyopathy, dilated 1EE (CMD1EE); Sick sinus syndrome 3 (SSS3)
Subcellular Location Cytoplasm, myofibril
Protein Families TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Database Links

HGNC: 7576

OMIM: 160710

KEGG: hsa:4624

STRING: 9606.ENSP00000348634

UniGene: Hs.278432

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