Recombinant Human N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD)

Code CSB-YP764247HU
MSDS
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Source Yeast
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Code CSB-EP764247HU
MSDS
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Source E.coli
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Code CSB-EP764247HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP764247HU
MSDS
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Source Baculovirus
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Code CSB-MP764247HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAPEPLD
Uniprot No.
Alternative Names
C7orf18; DKFZp781D1098; FMP30 ; Mbldc1; N acyl phosphatidylethanolamine hydrolyzing phospholipase D; N acyl phosphatidylethanolamine phospholipase D; N-acyl phosphatidylethanolamine phospholipase D; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; NAPE hydrolyzing phospholipase D; NAPE-hydrolyzing phospholipase D; NAPE-PLD; NAPEP_HUMAN; NAPEPLD
Species
Homo sapiens (Human)
Expression Region
1-393
Target Protein Sequence
MDENESNQSL MTSSQYPKEA VRKRQNSARN SGASDSSRFS RKSFKLDYRL EEDVTKSKKG KDGRFVNPWP TWKNPSIPNV LRWLIMEKDH SSVPSSKEEL DKELPVLKPY FITNPEEAGV REAGLRVTWL GHATVMVEMD ELIFLTDPIF SSRASPSQYM GPKRFRRSPC TISELPPIDA VLISHNHYDH LDYNSVIALN ERFGNELRWF VPLGLLDWMQ KCGCENVIEL DWWEENCVPG HDKVTFVFTP SQHWCKRTLM DDNKVLWGSW SVLGPWNRFF FAGDTGYCPA FEEIGKRFGP FDLAAIPIGA YEPRWFMKYQ HVDPEEAVRI HTDVQTKKSM AIHWGTFALA NEHYLEPPVK LNEALERYGL NAEDFFVLKH GESRYLNNDD ENF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs.
Gene References into Functions
  1. The AC genotype and C allele of NAPE-PLD rs12540583 locus are risk factors for schizophrenia. PMID: 29652995
  2. NAPE-PLD forms homodimers partly separated by an internal channel and uniquely adapted to associate with phospholipids. PMID: 25684574
  3. our results do not support a clear role of FAAH, CNR1 and NAPE-PLD in BD and lithium response. PMID: 24126189
  4. a major physiological role of NAPE-PLD PMID: 24018423
  5. The differential expression of NAPE-PLD and FAAH suggests that Anandamide could play an important role in the pathophysiology of preeclampsia. PMID: 23122699
  6. NAPE-PLD expression increase steadily after infancy, peaking in adulthood. PMID: 22827915
  7. The results of this study suggested that NAPEPLD was alterated in multip;e sclerosis. PMID: 21251115
  8. a common haplotype in NAPEPLD, an enzyme involved in endocannabinoid synthesis, was protective against obesity. PMID: 20885390
  9. Observational study of gene-disease association. (HuGE Navigator) PMID: 20885390
  10. During the menstrual cycle, NAPE-PLD immunoreactivity was down-regulated in the secretory epithelial gland compared to the proliferative epithelial gland and unaffected in the stroma. PMID: 20369362
  11. The human NAPE-PLD was activated by phosphatidylethanolamine and inhibited by the beta-lactamase substrate nitrocefin. PMID: 19715685

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Subcellular Location
Golgi apparatus membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Nucleus envelope. Nucleus, nucleoplasm.
Protein Families
NAPE-PLD family
Tissue Specificity
Widely expressed. Highest expression in brain, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).
Database Links

HGNC: 21683

OMIM: 612334

KEGG: hsa:222236

STRING: 9606.ENSP00000340093

UniGene: Hs.324271

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