Recombinant Human N-alpha-acetyltransferase 15, NatA auxiliary subunit (NAA15), partial

Code CSB-YP863143HU
MSDS
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Source Yeast
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Code CSB-EP863143HU
MSDS
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Source E.coli
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Code CSB-EP863143HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863143HU
MSDS
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Source Baculovirus
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Code CSB-MP863143HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAA15
Uniprot No.
Alternative Names
5730450D16Rik; 6330400I15; ASTBDN; GA19 ; Gastric cancer antigen Ga19; MGC29428; mNAT1; N alpha acetyltransferase 15, NatA auxiliary subunit; N terminal acetyltransferase; N(alpha) acetyltransferase 15, NatA auxiliary subunit ; N-alpha-acetyltransferase 15; N-terminal acetyltransferase; Naa15; NAA15_HUMAN; NatA auxiliary subunit; NATH ; NMDA receptor regulated 1; NMDA receptor regulated protein 1; NMDA receptor-regulated protein 1; Protein tubedown 1 ; Protein tubedown-1; Tbdn 1; Tbdn100; Transcriptional coactivator tubedown 100; tubedown-1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Auxillary subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase (NAT) activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
Gene References into Functions
  1. individuals with copy-number variant (CNV) deletions involving NAA15 and surrounding genes can present with mild intellectual disability, mild dysmorphic features, motor delays, and decreased growth. PMID: 29656860
  2. In combination with acetylation assays, the HypK N-terminal region is identified as a negative regulator of the NatA acetylation activity PMID: 28585574
  3. Authors highlight NAA15 as a novel candidate ID gene based on the vital role of NAA15 in the generation and differentiation of neurons in neonatal brain. PMID: 28990276
  4. Human Naa15 (NATH) and Naa10 (ARD1) form a stable NatA complex which associates with ribosomes and performs co-translational N-terminal acetylation; Naa15 (NATH) and Naa10 (ARD1) are cleaved during apoptosis resulting in decreased acetyltransferase activity PMID: 15496142
  5. Development of the first N-terminal acetyltransferase (NAT) inhibitors, including inhibitors to the NatA complex composed of Naa10 (ARD1) and Naa15 (NATH). PMID: 23557624
  6. Protein N-terminal acetyltransferases (NATs), including the NatA complex composed of Naa10 (ARD1) and Naa15 (NATH), act as N-terminal propionyltransferases. PMID: 23043182
  7. Data indicate that the physical interaction between HYPK and NatA Naa10/15 seems to be of functional importance both for huntingtin aggregation and for N-terminal acetylation. PMID: 20154145
  8. NATH, a novel gene overexpressed in papillary thyroid carcinomas. PMID: 12140756
  9. description of the human homologue of Nat1p, NATH (NAT human), as the binding partner of the hARD1 (human ARD1) protein PMID: 15496142
  10. levels of endogenous NATH and hARD1 proteins in thyroid papillary carcinoma patients; results suggest that NATH positively affects the level of hARD1 protein both in vivo and in cell cultures PMID: 16279846
  11. Loss of retinal endothelial Tbdn-1 expression may be a contributing factor in retinal blood vessel proliferation in retinopathy of prematurity. PMID: 16518308
  12. Essential role in cell survival through protein N-alpha-acetylation. PMID: 16518407
  13. NATH complexes with hARD1 in a ribosomal associated protein-N-acetyltransferase complex. PMID: 15496142

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Subcellular Location
Cytoplasm. Nucleus. Note=Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
Tissue Specificity
Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, u
Database Links

HGNC: 30782

OMIM: 608000

KEGG: hsa:80155

STRING: 9606.ENSP00000296543

UniGene: Hs.745047

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