Recombinant Human N-glycosylase/DNA lyase(OGG1)

Code CSB-EP016313HUa2
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names OGG1
Uniprot No. O15527
Research Area Cancer
Alternative Names 8 hydroxyguanine DNA glycosylase; 8 oxoguanine DNA glycosylase 1 ; 8 oxoguanine DNA glycosylase; AP lyase; DNA (apurinic or apyrimidinic site) lyase; DNA apurinic or apyrimidinic site lyase ; DNA lyase; DNA-(apurinic or apyrimidinic site) lyase; HMMH; HOGG 1; HOGG1; MMH; MUTM; N glycosylase; Ogg 1; Ogg1; OGG1_HUMAN; OGH 1; OGH1
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-345aa
Target Protein Sequence MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 54.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
Gene References into Functions
  1. study concludes that Ser326Cys polymorphism of the OGG1 gene may modulate the risk of colorectal cancer by increasing the level of oxidative DNA damage PMID: 29773766
  2. Results provide evidence that OGG1 rs1052133 is significantly associated with Wilms tumor susceptibility. PMID: 29937070
  3. TNF-alpha - Reactive Oxygen Species -driven enrichment of OGG1 at gene regulatory regions in the chromatinized DNA, which is a prerequisite to modulation of gene expression for prompt cellular responses to oxidant stress. PMID: 29940424
  4. OGG1 S326C polymorphism increases probability of AML relapse and may be useful as a prognostic factor for AML relapse risk. PMID: 29737460
  5. This study identifies patient's carrying the OGG1 Ser326Cys CC genotype are at greater risk for HIV associated low birth weight, and delivering prematurely. PMID: 29709520
  6. Of 39 subfertile men, 74.4% (29/39) had the hOGG1 Cys/Cys genotype. Patients in groups 1 and 2 with hOGG1 Cys/Cys genotype had significantly higher 8-OHdG content in sperm DNA, lower mitochondrial copy number in spermatozoa and lower TAC in seminal plasma than those with Ser/Ser or Ser/Cys genotype. PMID: 29582449
  7. CDK4/6 inhibitors also lead to accumulation of DNA damage by repressing PARP1 in oxidatively stressed cells. Thus, CDK4/6 inhibitors sensitize G1-arrested cells to anticancer drugs, since these cells require PARP1-OGG1 functional interaction for cell survival PMID: 29306194
  8. OGG1-2a, a critical enzyme for 8-OH-dG repair, is a direct target of miR-200a and its expression levels significantly decrease in aged keratinocytes. PMID: 29765508
  9. The level of OGG1 expression was significantly reduced in bipolar patients compared to healthy individuals, whereas the two groups exhibited similar levels of NEIL1 expression. PMID: 29626765
  10. Results identified a significant relationship between SNP rs13181 of OGG1 and an increased risk of endometrial cancer development. PMID: 30194171
  11. The OGG1 gene presents downregulation in the more advanced stages of colorectal cancer PMID: 29257843
  12. OGG1 gene Ser326Cys polymorphism was associated with age-related cataract risk (meta-analysis and review). PMID: 28631182
  13. Miners with the G/G genotype of the OGG1 (rs1052133) gene had an increased frequency of binucleated lymphocytes with micronuclei compared to the C/C genotype carriers. In addition, three-locus model of gene-gene interactions OGG1 (rs1052133) * ADPRT (rs1136410) * XRCC4 (rs6869366) was associated with high genotoxic risk in coal miners. PMID: 28992182
  14. Human Ogg1 removes 7,8-dihydro-8-oxoguanine (OG) paired with cytosine (C) but is inactive toward the OG:adenine (A) pair. In contrast, Geobacillus stearothermophilus Formamidopyrimidine-DNA Glycosylase removes OG from OG:C pairs and also exhibits appreciable (although diminished) activity toward OG:A pairs. PMID: 29320630
  15. For several turns of the helix, Authors observe that solution accessible 8-oxoGs are sites of activity for hOGG1. At the dyad axis, however, hOGG1 activity is suppressed, even at lesions predicted to be solution accessible by hydroxyl radical footprinting (HRF). PMID: 29341606
  16. The present meta-analysis concluded that the G allele was associated with an increased risk of cancer. It suggested that the hOGG1 polymorphism may be a candidate marker of cancer. PMID: 28415770
  17. hOGG1 mRNA is downregulated in non-small cell lung cancer tissues. Methylated +322-327 CpG site in the hOGG1 5'-UTR is associated with reduced expression of hOGG1 by decreasing the recruitment of Sp1 to the 5'-UTR of hOGG1 in A549 cells. PMID: 28586012
  18. uPA protection is independent of its catalytic activity, as the amino terminal fragment of uPA showed similar protection. A key enzyme for repairing oxidative DNA damage is the p53 target hOGG1. We found a significant increase of hOGG1 after pretreatment of HACM with uPA. Knockdown of hOGG1 completely abrogated the protective effect of uPA PMID: 28643198
  19. Study showed there was no association between hOGG1 rs1052133 and gastric cancer. [review] PMID: 28415729
  20. Ser326Cys variant effect on structural and functional properties of hOGG1 gene. PMID: 27822728
  21. SNP rs125701 in hOGG1 promoter was associated with the elevated gastric cancer risk. PMID: 27603140
  22. Provide strong evidence of an association between peripheral blood mononuclear cell hOGG1 methylation and the risk of non-small cell lung carcinoma in a Chinese population. PMID: 28039450
  23. HOGG1 Ser326Cys, APE1 Asp148Glu and XRCC1 Arg399Gln polymorphisms are correlated with the risk and clinicopathological features of PACG. PMID: 28396513
  24. The Gene expression levels of OGG1 was robustly elevated in oligodendrocytes laser captured from BA10 and amygdala white matter of Major Depressive Disorder. PMID: 28034960
  25. The findings showed that hOGG1-Ser326Cys Cys allele is statistically important and relevant with respect to the development of oral squamous cancer. PMID: 28448977
  26. Overexpressed beta-OGG1 has the same role as alpha-OGG1 in protecting bronchial epithelial cells from apoptosis and mitochondrial dysfunction. Additionally, knocking down OGG1 enhanced oxidative damage-induced apoptosis and mitochondrial dysfunction. The antiapoptotic function of beta-OGG1 involved the JNK signaling pathway. PMID: 29227732
  27. The rs2304277 variant in the OGG1 glycosidase gene associated to a significant OGG1 transcriptional down regulation independently of the BRCA mutational status and this variant may exert a synergistic effect together with BRCA1 or BRCA2 mutations in ovarian cancer PMID: 27015555
  28. OGG1 can initiate BER at positions off the dyad axis and that this activity is facilitated by spontaneous and transient unwrapping of DNA from the histones. Local nuances in the nucleosome environment and histone-DNA interactions can impact glycosylase activity. PMID: 28892740
  29. 8-oxoguanine DNA glycosylase-1 (OGG1) is the essential protein involved in oxidative stress-induced DNA demethylation. PMID: 27251462
  30. The allele combination of CGC from hOGG1, ITGA2 and XPD polymorphisms was significantly associated with increased odds of nasopharyngeal carcinoma. PMID: 29121049
  31. Increased oxidative DNA damage in primary open angle glaucoma may be attributed to decreased expression of DNA repair enzymes, OGG1 and PARP1, of the base excision repair pathway. PMID: 28129013
  32. Results show that some polymorphic variants in XRCC1 and OGG1 are associated with increased DNA damage in Alzheimer's Disease. PMID: 27011006
  33. The excision of the 8-oxoguanine base with DeltaG# = 16.1 kcal/mol proceeded via substitution of the C1-N9 N-glycosidic bond with an H-N9 bond where the negative charge on the oxoG base and the positive charge on the ribose were compensated in a concerted manner by NH3+(Lys249) and CO2-(Asp268), respectively. PMID: 28334993
  34. The interaction of OGG1 and XRCC1 DNA repair polymorphisms and arsenic exposure enhances telomeric DNA damage. PMID: 27084675
  35. These results together, points to a new paradigm about the role of DNA damage and repair by OGG1 in aging and age-associated disease processes. PMID: 27343030
  36. Enforced expression of hOGG1 and hAPE significantly protected thalamic neurons and motor neurons from retrograde apoptosis induced by target deprivation and axotomy. PMID: 27364693
  37. DNA repair protein OGG1 bound to its substrate is coupled to DNA occupancy of NF-kappaB and functions in epigenetic regulation of gene expression. PMID: 27756845
  38. Study shows increased level of 8-oxoG and decreased OGG1 protein in the serum of patients with Alzheimer's disease, suggesting that oxidative damage to DNA and a decreased DNA glycosylase are involved in the etiopathology of Alzheimer's disease PMID: 27538622
  39. APE1 Accelerates turnover of OGG1 by preventing retrograde binding to the abasic-site product. PMID: 28345889
  40. find that the energetic nature of the observed binding free energies of human 8-oxoguanine DNA glycosylase (hOGG1) and human uracil DNA glycosylase (hUNG) for undamaged DNA are derived from different sources. Although hOGG1 uses primarily nonelectrostatic binding interactions with nonspecific DNA, hUNG uses a salt-dependent electrostatic binding mode PMID: 27571472
  41. The Cys/Cys genotype of hOGG1 correlated strongly with the risk of developing ischemic cardiomyopathy. PMID: 27316582
  42. Polymorphisms of human 8-oxoguanine DNA glycosylase 1 and 8-hydroxydeoxyguanosine increase susceptibility to arsenic methylation capacity-related urothelial carcinoma. PMID: 26359225
  43. Polymorphisms in OGG1 gene is associated with gastrointestinal stromal tumours. PMID: 27460091
  44. Inherited OGG1 single nucleotide polymorphisms in Base-excision repair pathway are important determinants of oropharyngeal squamous cell carcinoma and predictors of patient outcomes. PMID: 27372710
  45. OGG1 and APE1 polymorphisms are associated with stage- and sex-specific risk of colorectal carcinoma in the Taiwanese population. PMID: 27022219
  46. The data of this study showed that OGG1 Ser326Cys and XRCC1 Arg399Gln gene polymorphisms had impacts on the development of stroke. PMID: 26936466
  47. remodeling by acetylation and dimethylation of lysine-14 and -4 residues of histone H3. In addition, OGG1 acts as a STAT1 coactivator and has transcriptional activity in the presence of endotoxin. The data presented here identifies a novel mechanism, and may provide new therapeutic strategies for the treatment of endotoxin-mediated inflammatory diseases. PMID: 26496208
  48. The frequency of OGG1 Ser326Cys polymorphisms was not significantly altered in Chinese patients with senile cataracts. PMID: 26884880
  49. This preliminary study suggests the possibility of different responses in DNA damage to the physical exercise training, considering the hOGG1 Ser326Cys polymorphism. PMID: 26592168
  50. OGG1 Ser/Cys and Ser/Cys + Cys/Cys genotypes had higher multiple sclerosis risk. PMID: 26562193
  51. study points at the elucidation of a possible association of Rheumatoid arthritis (RA) with Ser326Cys in OGG1 Arg194Trp and Arg399Gln polymorphisms of XRCC1 using a sample size of 100 patients and 100 controls from a Pakistani population PMID: 27393451
  52. Polymorphisms in OGG1 do not contribute to development of non-small cell lung cancer in Brazilian patients. PMID: 26400813
  53. our study in the Han Chinese population, along with the meta-analysis, failed to confirm the association of the hOGG1 gene Ser326Cys polymorphism with gastric cancer risk, even across different ethnic populations. PMID: 26600527
  54. Our results suggest that the circadian modulation of 8-oxoG DNA damage repair, according to a variation of Ogg1 expression, could render humans less susceptible to accumulate 8-oxoG DNA damage in the morning hours. PMID: 26337123
  55. XPC protein is required for OGG1 activity, but XPC does not interacts physically with OGG1. PMID: 26811994
  56. Authors demonstrate proof of concept that mtDNA damage results in breast cancer progression and metastasis in vivo. PMID: 26586787
  57. Results show that XRCC1-Arg/Gln, XRCC1-Arg/His, and OGG1 A/G polymorphism have a role in the development of rheumatoid arthritis disease. PMID: 26692147
  58. In the absence of hSSB1, human 8-oxoguanine glycosylase 1 does not localize to chromatin, resulting in the accumulation of 8-oxoguanine in the genome. PMID: 26261212
  59. A meta-analysis suggests that the hOGG1 C1245G polymorphism might be potentially associated with prostate cancer risk in different ethnicities and countries, especially among Asians. PMID: 25907663
  60. Our data indicated that the c.461G>A genetic variant of the OGG1 gene was associated with susceptibility to PC in a Chinese Han population. PMID: 25222230
  61. Results revealed an association between hOGG1 Ser326Cys polymorphism and the incidence of ovarian cancer. hOGG1 Ser326Cys and XRCC1 Arg194Trp polymorphisms may be regarded as risk factors of ovarian cancer PMID: 26124010
  62. ONIOM model suggests that the most likely mechanism of monofunctional hOgg1 action uses a water nucleophile and K249 acts as the general base that shuttles a proton from the nucleophile to the OG- leaving group PMID: 26018802
  63. A reduced OGG1 expression was correlated with hypermethylation of a CpG island of OGG1 in lens cortex of age-related cataract. PMID: 25310012
  64. A significant association was observed between OGG1 germ line mutations and breast cancer risk providing evidence that OGG1 may be a good candidate of better diagnosis, treatment, and prevention of breast cancer. [meta-analysis] PMID: 26089588
  65. hOGG1 induction in peripheral blood mononuclear cell of smokers COPD patients could be a biomarker of oxidative DNA damage in the lung. PMID: 25154311
  66. Results showed strong positive correlation between PARP1 and OGG1 in patients with colon cancer where both mRNA and protein levels were increased suggesting reciprocal influence on their expression and stability that may contribute to disease progression. PMID: 25526641
  67. our findings suggest that the hOGG1 c.269C > A and c.828A > G genetic variants are significantly associated with endometrial cancer susceptibility in Chinese Han populations and might be used as molecular markers for assessing the risk of EC. PMID: 25496161
  68. The OGG1 Ser326Cys polymorphism may be a risk factor for cancers of lung, digestive system and head and neck. PMID: 25588927
  69. Oxidative DNA damage increases in lenses with age-related cataract, and the three BER enzymes compensatively increase in the LECs, while decreasing in the opaque cortex. PMID: 24911554
  70. Results show that the C1245G polymorphism of hOGG1 may be one of the factors that confer the susceptibility to lupus nephritis and modulate the plasma level of 8-hydroxy-2'-deoxyguanosine in patients with systemic lupus erythematosus. PMID: 25671815
  71. the association between the OGG1 Ser326Cys polymorphism and the risk of cancer (Review) PMID: 23081862
  72. The association of OGG1 protein c.307G>C and c.828A>G single nucleotide polymorphisms with the risk of pancreatic cancer in Chinese Han population. PMID: 24935623
  73. These results suggest that OGG1-1b is associated with 8-oxoG cleavage in human mitochondria and that the mechanism of this repair is similar to that of nuclear OGG1-1a. PMID: 25196052
  74. Physiologically relevant levels of TNF-alpha simultaneously induce 8-oxodG and inactivate S326C-OGG1. It could be a mechanism that could contribute to increased risk of cancer among S326C-Ogg1 homozygous individuals. PMID: 25534136
  75. The polymorphisms rs1052133 on OGG1 and rs207454 on XDH show a trend of association with overall survival. PMID: 23443115
  76. The polymorphic XRCC1 variant R194W presents a defect in its interaction with the DNA glycosylase OGG1 after oxidative stress. PMID: 25733688
  77. OGG1 base excision repair has a role in the activation of small GTPases and changes in gene expression, including those that regulate pro-inflammatory chemokines/cytokines and cause inflammation. Review. PMID: 25250913
  78. Data indicate that both thymine-DNA glycosylase (hTDG) and a second glycosylase, hOGG1, recognized structurally different 8-oxoguanine lesions. PMID: 25712093
  79. OGG1 activity might be inhibited during postreplicative mismatch repair. PMID: 25694431
  80. In the Thai population, polymorphisms in OGG1, particularly in combination with XRCC1, are associated with increased susceptibility to cholangiocarcinoma (CCA), and they modify the effect of smoking and alcohol consumption in the risk of CCA. PMID: 25335960
  81. Variants in OGG1 could influence diabetes risk in Mexican American families. PMID: 20606456
  82. interaction of hOGG1 with specific and non-specific DNA-substrates PMID: 24911585
  83. These findings supported that hOGG1 Ser326Cys polymorphism may contribute to the susceptibility of digestive cancers PMID: 25323581
  84. OGG1 326 Ser/Cys and XPD 312 Asp/Asn heterozygous genotypes might be genetic markers modifying susceptibility to bladder cancer in Belarus. PMID: 25089939
  85. The polymorphism of DNA repair genes XPD, XRCC1, OGG1, and ERCC6, life expectancy, and the inclination to smoke PMID: 25731027
  86. findings have suggested that APE1, XRCC3, XPD, and hOGG1 gene variants could facilitate the development of migraine disease. PMID: 24892639
  87. hOGG1 Ser326Cys polymorphism might modify the susceptibility to colorectal cancer among the total population, especially among Caucasians. PMID: 25227662
  88. We investigated OGG1 expression changes in fetal membranes from spontaneous preterm birth and preterm premature rupture of the membranes and its changes in vitro in normal fetal membranes exposed to oxidative stress PMID: 24589083
  89. XRCC1 107 and 194, hOGG1, KCNQ2, and AT1R are not associated with endometriosis susceptibility. PMID: 22084859
  90. The hOGG1 Cys/Cys genotype may be a genetic susceptibility marker for NIHL in the Chinese Han population. PMID: 24599382
  91. Ser326Cys SNP was not associated with nasopharyngeal carcinoma risk in a Chinese cohort. PMID: 24175791
  92. Review/Meta-analysis: OGG1 Ser326Cys polymorphism may be a risk factor for breast cancer in Asians and postmenopausal patients. PMID: 24893568
  93. The combined results suggested that the hOGG1 Ser326Cys polymorphism was not associated with lung cancer susceptibility in different genetic models. PMID: 24435978
  94. The Cys allele was significantly associated with increased BMI, FBG level, and total cholesterol (TC) level. PMID: 24998955
  95. The OGG1 Cys allele provided a significant protective effect against developing cancer in women with a low BMI. PMID: 24606430
  96. These findings support a general mechanism where DNA glycosylases such as hOGG1 use highly dynamic multidimensional diffusion paths to scan DNA. PMID: 25016526
  97. To determine the genotypes and allele frequencies of XRCC1, XRCC3 Thr241Met and OGG1 gene polymorphisms. PMID: 23464476
  98. The Asn/Asn genotype and Asn(3)(1)(2) allele of XPD polymorphism, as well as the Cys/Cys genotype and Cys(3)(2) allele of the OGG1 polymorphism, may be associated with increased risk of the development of age-related cataract in the Egyptian population. PMID: 24868140
  99. results indicate a distinct molecular profile of polymorphisms for the DNA repair genes XRCC1, XRCC3, OGG1 and XPD loci for CRS compared to other populations. PMID: 23959014
  100. Cigarette smoking and hOGG1 Ser326Cys polymorphism are associated with 8-OHdG accumulation on mitochondrial DNA in thoracic esophageal squamous cell carcinoma. PMID: 22941157

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Involvement in disease Renal cell carcinoma (RCC)
Subcellular Location Nucleus, nucleoplasm, Nucleus speckle, Nucleus matrix
Protein Families Type-1 OGG1 family
Tissue Specificity Ubiquitous.
Database Links

HGNC: 8125

OMIM: 144700

KEGG: hsa:4968

UniGene: Hs.380271

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