Recombinant Human NACHT, LRR and PYD domains-containing protein 10 (NLRP10)

Code CSB-YP773044HU
MSDS
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Source Yeast
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Code CSB-EP773044HU
MSDS
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Source E.coli
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Code CSB-EP773044HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP773044HU
MSDS
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Source Baculovirus
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Code CSB-MP773044HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NLRP10
Uniprot No.
Alternative Names
CLR11.1; NACHT, leucine rich repeat and PYD containing 10; NACHT, LRR and PYD domains containing protein 10; NACHT, LRR and PYD domains-containing protein 10; NAL10_HUMAN; NALP10; NLR family, pyrin domain containing 10; Nlrp10; NOD8; Nucleotide-binding oligomerization domain protein 8; Nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 10; PAN5; PYNOD
Species
Homo sapiens (Human)
Expression Region
1-655
Target Protein Sequence
MAMAKARKPR EALLWALSDL EENDFKKLKF YLRDMTLSEG QPPLARGELE GLIPVDLAEL LISKYGEKEA VKVVLKGLKV MNLLELVDQL SHICLHDYRE VYREHVRCLE EWQEAGVNGR YNQVLLVAKP SSESPESLAC PFPEQELESV TVEALFDSGE KPSLAPSLVV LQGSAGTGKT TLARKMVLDW ATGTLYPGRF DYVFYVSCKE VVLLLESKLE QLLFWCCGDN QAPVTEILRQ PERLLFILDG FDELQRPFEE KLKKRGLSPK ESLLHLLIRR HTLPTCSLLI TTRPLALRNL EPLLKQARHV HILGFSEEER ARYFSSYFTD EKQADRAFDI VQKNDILYKA CQVPGICWVV CSWLQGQMER GKVVLETPRN STDIFMAYVS TFLPPDDDGG CSELSRHRVL RSLCSLAAEG IQHQRFLFEE AELRKHNLDG PRLAAFLSSN DYQLGLAIKK FYSFRHISFQ DFFHAMSYLV KEDQSRLGKE SRREVQRLLE VKEQEGNDEM TLTMQFLLDI SKKDSFSNLE LKFCFRISPC LAQDLKHFKE QMESMKHNRT WDLEFSLYEA KIKNLVKGIQ MNNVSFKIKH SNEKKSQSQN LFSVKSSLSH GPKEEQKCPS VHGQKEGKDN IAGTQKEAST GKGRGTEETP KNTYI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits autoprocessing of CASP1, CASP1-dependent IL1B secretion, PYCARD aggregation and PYCARD-mediated apoptosis but not apoptosis induced by FAS or BID. Displays anti-inflammatory activity. Required for immunity against C.albicans infection. Involved in the innate immune response by contributing to proinflammatory cytokine release in response to invasive bacterial infection. Contributes to T-cell-mediated inflammatory responses in the skin. Plays a role in protection against periodontitis through its involvement in induction of IL1A via ERK activation in oral epithelial cells infected with periodontal pathogens. Exhibits both ATPase and GTPase activities.
Gene References into Functions
  1. Authors observed an important role of NLRP10 in cytokine and chemokine production as well as expression of NF-kappaB and MAPKs in CSE-exposed A549 cells. Furthermore, replenishment of A549 cell culture with recombinant IL-17A (rIL-17A) during NLRP10 knockdown rescued CSE-induced inflammatory responses. PMID: 29501574
  2. these data suggest that NLRP10 is involved in activating the ERK signalling pathway in oral epithelial cell line infected with Tannerella forsythia and Fusobacterium nucleatum PMID: 28766990
  3. Review summarize recent advances of the role of of human and murine NLRP10 in innate and adaptive immune responses. [Review] PMID: 23562614
  4. These data revealed a novel role of NLRP10 in innate immune responses towards bacterial infection and suggest that NLRP10 functions as a scaffold for the formation of the NOD1-Nodosome. PMID: 22672233
  5. The results show that either deletion of the p53 binding elements within the PYNOD promoter or treatment with p53 inhibitor could significantly reduce PYNOD promoter activity. PMID: 22365966
  6. Transgenic PYNOD colocalizes with apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and inhibits caspase-1-mediated interleukin (IL)-1beta processing without inhibiting caspase-4-mediated caspase-1 processing. PMID: 20393137
  7. Observational study of gene-disease association. (HuGE Navigator) PMID: 20403135
  8. PYNOD is a novel regulator of apoptosis and inflammation. PMID: 15096476
  9. PYPAF3 is a feedback regulator of interleukin-1beta secretion, and PYPAF2 and PYPAF3, together with PYNOD, constitute an anti-inflammatory subgroup of PYRIN-containing apoptotic protease-activating factor-1-like proteins PMID: 15817483
  10. The structural modification of the NLRP10 gene might have played a role in development or enhancement of human-specific traits during evolution. PMID: 19544093

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein.
Protein Families
NLRP family
Tissue Specificity
Highly expressed in basal and suprabasal epidermal cell layers with lower levels in dermal fibroblast cells (at protein level). Widely expressed with highest levels in heart, brain and skeletal muscle. Also expressed in liver, colon, dermis and epidermis.
Database Links

HGNC: 21464

OMIM: 609662

KEGG: hsa:338322

STRING: 9606.ENSP00000327763

UniGene: Hs.449636

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