Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
Adrenodoxin reductase; ADRO_HUMAN; ADXR; AR; FDXR; Ferredoxin NADP(+) reductase; Ferredoxin reductase; Ferredoxin--NADP(+) reductase; mitochondrial; NADPH adrenodoxin oxidoreductase mitochondrial; NADPH:adrenodoxin oxidoreductase
Species
Homo sapiens (Human)
Expression Region
33-451aa
Target Protein Sequence
STQEKTPQICVVGSGPAGFYTAQHLLKQHPQAHVDIYEKQPVPFGLVRFGVAPDHPEVKSYGAEDHRALEIPGEELPGVCSARAFVGWYNGLPENQELEPDLSCDTAVILGQGNVALDVARILLTPPEHLERTDITKAALGVLRQSRVKTVWLVGRRGPLQVAFTIKELREMIQLPGARPILDPVDFLGLQDKIKEVPRPRKRLTELLLRTATEKPGPAEAARQASASRAWGLRFFRSPQQVLPSPDGRRAAGVRLAVTRLEGVDEATRAVPTGDMEDLPCGLVLSSIGYKSRPVDPSVPFDSKLGVIPNVEGRVMDVPGLYCSGWVKRGPTGVIATTMTDSFLTGQMLLQDLKAGLLPSGPRPGYAAIQALLSSRGVRPVSFSDWEKLDAEEVARGQGTGKPREKLVDPQEMLRLLGH
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The preparation of this recombinant Human FDXR protein was to use gene recombination DNA technology to obtain a recombinant vector connected with a FDXR fragment (33-451aa) that could be translated into the FDXR protein and then transferred it into E.coli cells to express the recombinant FDXR protein molecule. In order to get the target protein with high purity, N-terminal 6xHis-SUMO tag was used in the production. The purity is 90% determined by SDS-PAGE.
Ferredoxin reductase (FDXR, also known as adrenodoxin reductase) is a mitochondrial flavoprotein and functions as the first electron transfer protein of mitochondrial P450 systems such as P450scc. It is a mitochondrial flavoprotein that initiates electron transport from NADPH to several cytochromes P450 via two electron carriers, ferredoxin 1 (FDX1) and FDX2. Functionally, FDXR is suggested to be involved in various metabolic processes, including steroidogenesis, heme and iron-sulfur cluster biosynthesis. Notably, recent studies have shown that FDXR mutations are associated with mitochondrial disorders, probably due to their role in iron-sulfur cluster protein biosynthesis. In addition, FDXR has also been found to be a sensitive and reliable biomarker of radiation exposure in vivo. FDXR could regulate TP73 tumor suppressor via IRP2 to modulate aging and tumor suppression. Mutation in FDXR gene is associated with Sensorial Neuropathies. Abundant FDXR expression in these steroidogenic cells was maintained through SF-1 binding to the intronic enhancer of the FDXR gene.
