Recombinant Human NAD (P)H-hydrate epimerase (NAXE)

Code CSB-YP839855HU
MSDS
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Source Yeast
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Code CSB-EP839855HU
MSDS
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Source E.coli
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Code CSB-EP839855HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839855HU
MSDS
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Source Baculovirus
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Code CSB-MP839855HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAXE
Uniprot No.
Alternative Names
NAXE; AIBP; APOA1BP; YJEFN1; NAD(P)H-hydrate epimerase; EC 5.1.99.6; Apolipoprotein A-I-binding protein; AI-BP; NAD(P)HX epimerase; YjeF N-terminal domain-containing protein 1; YjeF_N1
Species
Homo sapiens (Human)
Expression Region
48-288
Target Protein Sequence
DSE VMASTVVKYL SQEEAQAVDQ ELFNEYQFSV DQLMELAGLS CATAIAKAYP PTSMSRSPPT VLVICGPGNN GGDGLVCARH LKLFGYEPTI YYPKRPNKPL FTALVTQCQK MDIPFLGEMP AEPMTIDELY ELVVDAIFGF SFKGDVREPF HSILSVLKGL TVPIASIDIP SGWDVEKGNA GGIQPDLLIS LTAPKKSATQ FTGRYHYLGG RFVPPALEKK YQLNLPPYPD TECVYRLQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Accelerates cholesterol efflux from endothelial cells to high-density lipoprotein (HDL) and thereby regulates angiogenesis.
Gene References into Functions
  1. NAXE gene mutations are associated with early-onset progressive encephalopathy. PMID: 30022751
  2. Study shows a homozygous mutation in the APOA1BP is associated with a lethal infantile leukoencephalopathy.This is the first report of a defect in the nicotinamide nucleotide repair system in humans. PMID: 27122014
  3. NAXE Mutations Disrupt the Cellular NAD(P)HX Repair System and Cause a Lethal Neurometabolic Disorder of Early Childhood PMID: 27616477
  4. AIBp contributes to mitotic entry and bipolar spindle assembly. PMID: 26114227
  5. AIBP accelerates cholesterol efflux from endothelial cells to HDL and thereby regulates angiogenesis PMID: 23719382
  6. Taken together, the current study demonstrates that APN might protect against atherosclerosis by increasing HDL assembly through enhancing ABCA1 pathway and apoA-1 synthesis in the liver. PMID: 17521614

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Involvement in disease
Encephalopathy, progressive, early-onset, with brain edema and/or leukoencephalopathy (PEBEL)
Subcellular Location
Mitochondrion. Secreted.
Protein Families
NnrE/AIBP family
Tissue Specificity
Ubiquitously expressed, with highest levels in kidney, heart and liver. Present in cerebrospinal fluid and urine but not in serum from healthy patients. Present in serum of sepsis patients (at protein level).
Database Links

HGNC: 18453

OMIM: 608862

KEGG: hsa:128240

STRING: 9606.ENSP00000357218

UniGene: Hs.528320

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