Recombinant Human Neurogenin-3 (NEUROG3)

Code CSB-YP896521HU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP896521HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP896521HU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP896521HU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
NEUROG3
Uniprot No.
Alternative Names
ATH4B; Atoh5; bHLHa7; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; Neurogenin-3; Neurogenin3; NGN-3; ngn3; NGN3_HUMAN; OTTHUMP00000019730; Protein atonal homolog 5
Species
Homo sapiens (Human)
Expression Region
1-214
Target Protein Sequence
MTPQPSGAPT VQVTRETERS FPRASEDEVT CPTSAPPSPT RTRGNCAEAE EGGCRGAPRK LRARRGGRSR PKSELALSKQ RRSRRKKAND RERNRMHNLN SALDALRGVL PTFPDDAKLT KIETLRFAHN YIWALTQTLR IADHSLYALE PPAPHCGELG SPGGSPGDWG SLYSPVSQAG SLSPAASLEE RPGLLGATFS ACLSPGSLAF SDFL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types.
Gene References into Functions
  1. novel homozygous nonsense mutation (p.Q4*) as the genetic cause of neonatal diabetes mellitus and severe malabsorptive diarrhea in 2 cousins PMID: 28940958
  2. PDX1, Neurogenin-3, and MAFA are critical transcription regulators for beta cell development and regeneration. (Review) PMID: 29096722
  3. Sox9 and Ngn3, key transcription factors associated with pancreatic development. PMID: 27836003
  4. This reviews the expression and function of NEUROG3 in both mouse and human pancreatic development. [Review Article] PMID: 27615127
  5. Neurogenin3 controls its ability to promote pancreatic endocrine differentiation and to maintain beta cell function in the presence of pro-proliferation cues PMID: 28457793
  6. Phosphorylation of NEUROG3 links endocrine differentiation to the cell cycle in pancreatic progenitors PMID: 28441528
  7. Collectively, our results demonstrate that the STAT3(K392R) mutation causes premature endocrine differentiation through direct induction of NEUROG3 expression. PMID: 28402852
  8. inflammatory cytokine insults stimulate epithelial-to-mesenchymal transition (EMT) as well as the endocrine program in human pancreatic ductal cells via STAT3-dependent NGN3 activation. PMID: 27068459
  9. ChIP experiments confirmed that Pdx1 activates the expression of the downstream transcription factors, Ngn3 and Pax6, by combined with the promoter regions of insulin (Insulin-P), Ngn3 (Ngn3-P), and Pax6 (Pax6-P). PMID: 26345820
  10. NGN3 expression in the adult human exocrine pancreas marks a dedifferentiating cell population. PMID: 26288179
  11. conclude that NEUROG3 is essential for endocrine pancreas development in humans and that as little as 10% NEUROG3 is sufficient for formation of pancreatic endocrine cells PMID: 25650326
  12. NEUROG3 deficiency produces a rare clinical syndrome characterised by severe malabsorptive diarrhoea from early life and mild diabetes with a variable age of onset. PMID: 25120094
  13. Activation of the developmental pathway neurogenin-3/microRNA-7a regulates cholangiocyte proliferation in response to injury. PMID: 24925797
  14. The expression of transcription factor Ngn3 and pancreatic mesenchymal microenvironment are important and necessary to promote pancreatic progenitors differentiated to islet cells regardless of pancreatic development or islets regeneration. PMID: 24969979
  15. Recessively inherited NEUROG-3 mutations were originally identified in three patients with unexplained congenital malabsorptive diarrhea and an absence of EC. PMID: 24134759
  16. Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells. PMID: 24493854
  17. Data indicate associations of SNPs in eight loci CXCR4, HHEX, FOXA2, NGN3, TCF7L2, FLJ39370 (C4orf32), LOC646279 (RPL21P7) and THADA with body mass index (BMI) and weight. PMID: 23349771
  18. Ngn3-mediated pancreatic duct-to-endocrine cell reprogramming was measured employing genome wide mRNA profiling. PMID: 22606327
  19. CCAR1 is a novel partner of Ngn3 in mediating endocrine differentiation. PMID: 22266316
  20. The variants in HNFA and NEUROG3 indicate a strong predisposition of normal-weight/lean subjects to T2D in North Indians. PMID: 21814221
  21. Gastrin-positive neuroendocrine tumors, whether of duodenal or pancreatic origin, frequently expressed PDX1 (17/18), ISL1 (14/18), and NGN3 (14/18). PMID: 21739268
  22. Severe deficiency of neurogenin 3 causes a rare novel subtype of permanent neonatal diabetes. This finding confirms the essential role of NEUROG3 in islet development and function in humans PMID: 21378176
  23. Ngn3-Cre-based lineage tracing showed that pdx-1-expressing cells differentiated to all the types of pancreatic cells, while Ngn3 marked endocrine-specific progenitors. PMID: 20668890
  24. The important role of Ngn3 as a master regulator of endocrine pancreas development directs attention to finding therapeutic approaches to enhance Ngn3 expression in diabetes as a means to increase beta cell mass and functions. PMID: 21099270
  25. These results support an important inter-species difference in regulating insulin exocytosis where RAB3B is the most expressed isoform in human islets. PMID: 20807725
  26. mutations of NEUROG3 gene are not a common cause of neonatal/infancy/childhood-onset diabetes PMID: 19538245
  27. adult human pancreatic duct cells can be converted into insulin-expressing cells after ectopic, adenovirus-mediated expression of the class B basic helix-loop-helix factor neurogenin 3 PMID: 12403815
  28. Polymorphism contributes to glucose intolerance in a South Indian population. PMID: 15277395
  29. The Notch/Ngn3 signalling network is intact and functional in adult islets. PMID: 17922104
  30. A genetic variation in the NGN3 gene may be among the genetic determinants involved in the pathogenesis of diabetes. PMID: 18072012
  31. Tumors and MEN1 nontumorous endocrine cells showed a prominent cytoplasmatic NEUROG3 and NEUROD1 expression PMID: 19307926

Show More

Hide All

Involvement in disease
Diarrhea 4, malabsorptive, congenital (DIAR4)
Subcellular Location
Nucleus.
Database Links

HGNC: 13806

OMIM: 604882

KEGG: hsa:50674

STRING: 9606.ENSP00000242462

UniGene: Hs.532682

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1