Recombinant Human Nuclear RNA export factor 5 (NXF5)

Code CSB-YP016223HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP016223HU
MSDS
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Source E.coli
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Code CSB-EP016223HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016223HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP016223HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NXF5
Uniprot No.
Alternative Names
NXF5; TAPL1; Nuclear RNA export factor 5; TAP-like protein 1; TAPL-1
Species
Homo sapiens (Human)
Expression Region
1-397
Target Protein Sequence
MRRNTQDENM RKWFKVTIPY GIKYDKAWLM NSIQSNCSVP FTPVDFHYIR NRACFFVQVA SAASALKDVS YKIYDDENQK ICIFVSHFTA PYSVKNKLKP GQMEMLKLTM NKRYNVSQQA LDLQNLRFDP DLMGRDIDII LNRRNCMAAT LKITERNFPE LLSLNLCNNK LYQLDGLSDI TEKAPKVKTL NLSKNKLESA WELGKVKGLK LEELWLEGNP LCSTFSDQSA YVSAIRDCFP KLLRLDGREL SAPVIVDIDS SETMKPCKEN FTGSETLKHL VLQFLQQSNL CKYFKDSRNI KILKDPYLQR KLLKHTKCPR NVDSLSALPE TQHDFTSILV DMWYQTVNTC FLPRAGPESQ RWWCLLSLKW KDGLRVLILP SCGPSSLPLA AIPVCAS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Could be involved in the export of mRNA from the nucleus to the cytoplasm. Could also have a role in polarized cytoplasmic transport and localization of mRNA in neurons.
Gene References into Functions
  1. study of an Australian pedigree in which focal segmental glomerulosclerosis co-segregates with progressive heart block; study found 2 mutations NXF5-R113W and ALG13-T141L segregated with disease phenotype; predicted the NXF5 R113W mutation to be deleterious; cellular studies support a role in the stability and localization of the protein suggesting a causative role PMID: 23686279
  2. Mutations of the NXF5 gene is associated with X-linked mental retardation PMID: 12784308
Involvement in disease
A chromosomal aberration involving NXF5 has been observed in one patient with a syndromic form of mental retardation and short stature. Pericentric inversion inv(X)(p21.1;q22) that interrupts NXF5.
Subcellular Location
Cytoplasm. Nucleus. Note=Mainly localized in the cytoplasm of cells and more particularly in the cell body and neurites of hippocampal neurons. Although nuclear localization is also observed. Not detected at nuclear rim.
Protein Families
NXF family
Database Links

HGNC: 8075

OMIM: 300319

KEGG: hsa:55998

STRING: 9606.ENSP00000426978

UniGene: Hs.307077

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