Recombinant Human Nuclear pore complex protein Nup133 (NUP133), partial

Code CSB-YP855476HU
MSDS
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Source Yeast
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Code CSB-EP855476HU
MSDS
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Source E.coli
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Code CSB-EP855476HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP855476HU
MSDS
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Source Baculovirus
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Code CSB-MP855476HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NUP133
Uniprot No.
Alternative Names
133 kDa nucleoporin; FLJ10814; hNUP 133; hNUP133; MGC21133; NU133_HUMAN; Nuclear pore complex protein Nup133; Nucleoporin 133kDa; Nucleoporin NUP133; NUP 133; NUP133; OTTHUMP00000061095
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in poly(A)+ RNA transport. Involved in nephrogenesis.
Gene References into Functions
  1. human Nup133 contains two domains: a COOH-terminal domain responsible for its interaction with its subcomplex through Nup107; and an NH2-terminal domain whose crystal structure reveals a seven-bladed beta-propeller. PMID: 15557116
  2. When complexed with NUP107, this complex will give the first insights into the protein-protein interactions within a core module of the nuclear pore complex. PMID: 17768364
  3. The significant topological differences between Nup107 and Nup133 suggest that helical nucleoporin domains of the nuclear pore complex scaffold fall in different classes and fulfill largely nonredundant functions. PMID: 18570875
  4. Data present crystal structures of yNup170(979-1502) and hNup107(658-925) x hNup133(517-1156), and conservation of domain arrangement and of tertiary structure suggests that Nup157/170 and Nup133 derived from a common ancestor. PMID: 19674973
Subcellular Location
Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore.
Protein Families
Nucleoporin Nup133 family
Tissue Specificity
Widely expressed in fetal and adult tissues. Expressed in the brain and kidney.
Database Links

HGNC: 18016

OMIM: 607613

KEGG: hsa:55746

STRING: 9606.ENSP00000261396

UniGene: Hs.12457

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