Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
(Sec)(Liver-pancreas antigen)(LP)(SLA-p35)(SLA/LP autoantigen)(Selenocysteine synthase)(Sec synthase)(Selenocysteinyl-tRNA(Sec)(Sep-tRNA:Sec-tRNA synthase)(SepSecS)(Soluble liver antigen)(SLA)(UGA suppressor tRNA-associated protein)(tRNA(Ser/Sec)
Species
Homo sapiens (Human)
Expression Region
1-501aa
Target Protein Sequence
MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDASS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The production process of the recombinant human O-phosphoseryl-tRNA (Sec) selenium transferase (SEPSECS) involves the expression of a vector construct in yeast cells. To create this construct, the gene fragment encoding the full-length human SEPSECS protein (1-501aa) is cloned with a C-terminal 6xHis-tag into a plasmid vector. The resulting vector construct, harboring the desired gene, is then transfected into yeast cells. Transfected yeast cells are carefully selected using specific antibiotics and subsequently cultured under optimal conditions. The induction reagents aid in the expression of the recombinant human SEPSECS protein within the yeast cells. After expression, the yeast cells are harvested and lysed to release their cellular contents, including the recombinant SEPSECS protein. Purification of the recombinant SEPSECS protein is conducted from the cell lysate, yielding a purity level exceeding 85% as confirmed by SDS-PAGE analysis.
