Recombinant Human OTU domain-containing protein 1 (OTUD1)

Code CSB-YP733158HU
MSDS
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Source Yeast
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Code CSB-EP733158HU
MSDS
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Source E.coli
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Code CSB-EP733158HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733158HU
MSDS
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Source Baculovirus
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Code CSB-MP733158HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
OTUD1
Uniprot No.
Alternative Names
DUBA-7; DUBA7; OTDC1; OTU domain containing 1; OTU domain-containing protein 1; OTUD1; OTUD1_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-481
Target Protein Sequence
MQLYSSVCTH YPAGAPGPTA AAPAPPAAAT PFKVSLQPPG AAGAAPEPET GECQPAAAAE HREAAAVPAA KMPAFSSCFE VVSGAAAPAS AAAGPPGASC KPPLPPHYTS TAQITVRALG ADRLLLHGPD PVPGAAGSAA APRGRCLLLA PAPAAPVPPR RGSSAWLLEE LLRPDCPEPA GLDATREGPD RNFRLSEHRQ ALAAAKHRGP AATPGSPDPG PGPWGEEHLA ERGPRGWERG GDRCDAPGGD AARRPDPEAE APPAGSIEAA PSSAAEPVIV SRSDPRDEKL ALYLAEVEKQ DKYLRQRNKY RFHIIPDGNC LYRAVSKTVY GDQSLHRELR EQTVHYIADH LDHFSPLIEG DVGEFIIAAA QDGAWAGYPE LLAMGQMLNV NIHLTTGGRL ESPTVSTMIH YLGPEDSLRP SIWLSWLSNG HYDAVFDHSY PNPEYDNWCK QTQVQRKRDE ELAKSMAISL SKMYIEQNAC S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin.
Gene References into Functions
  1. Report that YAP is subject to non-proteolytic, K63-linked polyubiquitination by the SCF(SKP2) E3 ligase complex (SKP2), which is reversed by the deubiquitinase OTUD1. The non-proteolytic ubiquitination of YAP enhances its interaction with its nuclear binding partner TEAD, thereby inducing YAP's nuclear localization, transcriptional activity, and growth-promoting function. PMID: 29891922
  2. we report the establishment of OTUD1-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method. The generated antibodies recognize the N-terminal portion (aa. 1-290) of human and mouse OTUD1 proteins PMID: 30130141
  3. OTU deubiquitinase 1 (OTUD1) directly deubiquitinates Smad7 Protein (SMAD7) and prevents its degradation. PMID: 29235476
  4. these results suggest that OTUD1 is a novel regulator of p53 stability and activity. PMID: 28216291
  5. OTU domain-containing protein 1 deubiquitinating enzyme is differentially expressed in thyroid cancer. PMID: 24937664

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Database Links

HGNC: 27346

OMIM: 612022

KEGG: hsa:220213

STRING: 9606.ENSP00000365678

UniGene: Hs.499042

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