Recombinant Human Oxysterols receptor LXR-alpha(NR1H3)

Code CSB-YP619753HU
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Source Yeast
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Code CSB-EP619753HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619753HU
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Source Baculovirus
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Code CSB-MP619753HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NR1H3
Uniprot No. Q13133
Alternative Names Liver X receptor alpha; LXR a; LXRA; NR1H3; NR1H3_HUMAN; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR-alpha; RLD 1; RLD1
Species Homo sapiens (Human)
Expression Region 1-447
Target Protein Sequence MSLWLGAPVP DIPPDSAVEL WKPGAQDASS QAQGGSSCIL REEARMPHSA GGTAGVGLEA AEPTALLTRA EPPSEPTEIR PQKRKKGPAP KMLGNELCSV CGDKASGFHY NVLSCEGCKG FFRRSVIKGA HYICHSGGHC PMDTYMRRKC QECRLRKCRQ AGMREECVLS EEQIRLKKLK RQEEEQAHAT SLPPRASSPP QILPQLSPEQ LGMIEKLVAA QQQCNRRSFS DRLRVTPWPM APDPHSREAR QQRFAHFTEL AIVSVQEIVD FAKQLPGFLQ LSREDQIALL KTSAIEVMLL ETSRRYNPGS ESITFLKDFS YNREDFAKAG LQVEFINPIF EFSRAMNELQ LNDAEFALLI AISIFSADRP NVQDQLQVER LQHTYVEALH AYVSIHHPHD RLMFPRMLMK LVSLRTLSSV HSEQVFALRL QDKKLPPLLS EIWDVHE
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators.
Gene References into Functions
  1. The authors report that, in Mycobacterium tuberculosis-infected macrophages, IL-36 signaling modulates cholesterol biosynthesis and efflux via LXR. PMID: 29367626
  2. LXR-alpha played a central role in down-regulating of ABCA1 and ABCG1 and lipid accumulation induced by homocysteine in the macrophages. PMID: 30393020
  3. LXRalpha interacts with OGT in its N-terminal domain and ligand-binding domain (LBD) in a ligand-independent fashion. PMID: 29577901
  4. Our data suggest that LXR could be used as a biomarker for hepatocellular carcinoma prognosis. PMID: 28508927
  5. PBMCs from healthy persons were predisposed to the MPhi2 differentiation phenotype, which exhibits elevated cholesterol uptake and anti-inflammatory properties. LXRalpha over-expression polarizes macrophages towards the anti-inflammatory MPhi2 phenotype. PMID: 29420090
  6. LXR gene expression was significantly increased in obese children with obstructive sleep apnea-hypopnea syndrome (OSAHS). The severity of OSAHS was positively correlated with COX-2 levels. PMID: 28676625
  7. In conclusion, the present study demonstrated that activation of LXRalpha-ABCA1 axis with a synthetic LXR agonist TO90 exerted a potent protective effect against Abeta induced senescent and inflammatory responses in retinal pigment epithelial cells, suggesting that LXR agonists may be promising therapeutic agents for treating age-related macular degeneration. PMID: 28361293
  8. AMPK activates LXRalpha and ABCA1 expression in human macrophages PMID: 27343431
  9. PPARalpha and LXRalpha may be mediators by which omega3PUFA attenuate bile acid-induced hepatocellular injury PMID: 26756785
  10. Inhibition of Pancreatic Cancer Cell-Induced Paracrine Hedgehog Signaling by Liver X Receptor Agonists and Oxy16, a Naturally Occurring Oxysterol PMID: 27490478
  11. Data identify LXR as an important factor in early-pregnancy lipogenesis that is also necessary to protect against abnormalities in fetoplacental lipid homeostasis. PMID: 28420650
  12. data suggest that ASXL3 is another corepressor of LXRalpha, promoting to the regulation of lipid homeostasis PMID: 25450400
  13. results indicated that LXRalpha plays a specific and important role in activation of TH by regulating D1, and that LXRalpha binds to and regulates the hDIO1 promoter, competing with TRbeta on specific sequences within the promoter. PMID: 28617824
  14. GW3965 significantly increases the expression of liver X nuclear receptor beta (LXRbeta) mRNA, while the liver X nuclear receptor alpha (LXRalpha( mRNA expression did not change a lot, and sensitizes gefitinib by inhibiting NF-kappa B (NF-kappaB) activation. PMID: 28178657
  15. Transactivation assays showed that MCFA activated LXRa, whereas long-chain FA caused no effect. Our results suggest that LXRa functions as a receptor for saturated FA or acyl-CoA of C10 and C12 in length. PMID: 28011707
  16. We demonstrated that LXR stimulation decreases mRNA and protein expression of FLOT2 and DHHC5 in MCF-7 cells. LXR stimulation also reduces Akt phosphorylation and its localization at the plasma membrane PMID: 28739689
  17. The effects of LXR agonist on interleukin-8 (IL-8) secretion and nuclear factor-kappa B (NF-kappaB) activation in human umbilical vein endothelial cells (HUVECs), is reported. PMID: 27489081
  18. Collectively, these findings demonstrate that LXRalpha activation induces 17beta-HSD13 expression in a SREBP-1c-dependent manner. PMID: 28270440
  19. mutant NR1H3 (LXRA) alters gene expression profiles, suggesting a disruption in transcriptional regulation as one of the mechanisms underlying Multiple Sclerosis pathogenesis. PMID: 27253448
  20. Data show that the synthetic liver X receptors (LXRs) agonist T0901317 promoted cytokines IL-1beta, IL-6 and TNFalpha mRNA degradation, destabilized TNFalpha mRNA through its 3'-untranslated region, and increased the expression of tristetraprolin (TTP). PMID: 28119310
  21. RXRalpha negatively regulates the transcription and expression by directly binding to the RARE in the promoter of Cx43 PMID: 26991262
  22. Serum and placental LXR-alpha and endoglin levels were significantly higher in patients with preeclampsia than those in control group (P<0.05, each). PMID: 27736929
  23. this study shows that the anti-inflammatory effect of platycodin D in IL-1beta-stimulated chondrocytes is mediated by activating LXR-a PMID: 27743553
  24. results indicated that down-regulation of LXRalpha was shown to suppress invasion and EMT of gastric cancer cells PMID: 28091828
  25. this study shows that show that H2O2 exerts a dual regulation on mRNA expression of LXRalpha and its target genes PMID: 27351826
  26. combined PPARgamma C1431T, PGC-1alpha G482S, and LXRalpha -115G/A polymorphisms increase the risk of coronary artery disease and predicted the severity of coronary atherosclerosis in Thais PMID: 27016616
  27. Distinct gene regulatory programs define the inhibitory effects of liver X receptors, NR1H2/NR1H3 and PPARG on cancer cell proliferation. PMID: 27401066
  28. these data show that YXS is effective in mitigating MI/R injury by suppressing mitochondrial mediated apoptosis and oxidative stress and by upregulating LXRalpha, thereby providing a rationale for future clinical trials and clinical applications PMID: 26964694
  29. decreased expression of miR-155 in the peripheral blood may be utilized as a potential novel biomarker for non-alcoholic fatty liver disease screening mainly by targeting LXRalpha. PMID: 27832630
  30. The anti-inflammatory effects of platelet-derived microparticles in human plasmacytoid dendritic cells involve liver X receptor activation. PMID: 26635040
  31. these data identify a new mechanism of LXR regulation that involves TIPARP, ADP-ribosylation and MACROD1. PMID: 26814197
  32. Intestinal activation of LXR reduces the production of chylomicrons by a mechanism dependent on the apical localization of SR-B1. PMID: 26602218
  33. Suggest that blocking cholesterol deposition and inhibiting the LXRalpha pathway-induced inflammatory response might be one of the main mechanisms by which anthocyanins exert their protective effects in diabetic nephropathy. PMID: 26379423
  34. LXR-alpha might downregulate S1PR2 expression via miR-130a-3p in quiescent HUVECs. Stimulation of TNF-alpha attenuates the activity of LXR-alpha and results in enhanced S1PR2 expression. PMID: 26669941
  35. Lipoxin A4 increases ABCA1 expression and promotes cholesterol efflux through LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID: 26261553
  36. These results identify LXRalpha as a key cardiac transcriptional regulator that helps orchestrate an adaptive metabolic response to chronic cardiac stress. PMID: 26160456
  37. Studied the role of LXRalpha with Wnt/beta-catenin signaling in adipogenesis of MSCs. PMID: 26595172
  38. In conclusion, our data indicate that HNF-4alpha may have a wider role in cell and plasma cholesterol homeostasis by controlling the expression of LXRalpha in hepatic cells. PMID: 26692490
  39. Propofol up-regulates expression of ABCA1, ABCG1, and SR-B1 through the PPARgamma/LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID: 25600616
  40. The rs12221497 polymorphism in the LXRalpha gene was associated with the susceptibility to stroke in a Han Chinese population. PMID: 25867319
  41. Data show that menin, encoded by the MEN1 gene, inhibits the transcriptional activity of nuclear receptor liver X receptor alpha (LXRalpha). PMID: 25962847
  42. the rsl2221497 polymorphism in the LXRalphagene was associated with the susceptibility to stroke in a Chinese population. PMID: 25729942
  43. treatment with Ang-(1-7) promoted cholesterol efflux in Ang II-treated THP-1 macrophages, partly through inactivation of p38 and JNK signaling and by inducing the expression of PPARg and LXRa. PMID: 25779847
  44. LXRb is the dominant isoform in the rat myocardium and the expression of both LXR isoforms (LXRa and LXRb) did not change after administration of T0901317 PMID: 25659329
  45. The data indicate a direct inhibitory interaction of polyunsaturated fatty acids with LXRalpha, a consequent reduction of SREBP-1 and of its binding to SCD1 promoter. PMID: 25264165
  46. NR1H3 accelerates hepatic differentiation through an HNF4alpha-dependent reciprocal network. PMID: 25073010
  47. This study provides the first evidence to show LXR activation reduces cadmium-induced apoptotic cell death of human renal proximal tubular cells by inhibition of reactive oxygen species production and JNK activation. PMID: 25980575
  48. Activation of LXRs interfered with the release of interleukin-6 from macrophages and, thus, inhibited fibroblast activation and collagen release. PMID: 24618263
  49. ligands selectively regulate placenta gene targets and functional pathways PMID: 25255963
  50. An increase of 55% in LXR-alpha gene expression at RNA level was observed in Atorvastatin + 22-R hydroxycholestrol compared to 24% in Ascorbic acid + 22-ROH cholesterol. PMID: 25283515

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Subcellular Location Nucleus. Cytoplasm.
Protein Families Nuclear hormone receptor family, NR1 subfamily
Tissue Specificity Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
Database Links

HGNC: 7966

OMIM: 602423

KEGG: hsa:10062

STRING: 9606.ENSP00000387946

UniGene: Hs.438863

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