Recombinant Human PRELI domain-containing protein 1, mitochondrial (PRELID1)

Code CSB-YP896867HU
MSDS
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Source Yeast
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Code CSB-EP896867HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896867HU
MSDS
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Source Baculovirus
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Code CSB-MP896867HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRELID1
Uniprot No.
Alternative Names
25 kDa protein of relevant evolutionary and lymphoid interest; CGI 106; MGC87972; mitochondrial; PRELI; PRELI domain containing 1; PRELI domain containing protein 1; PRELI domain containing protein 1 mitochondrial; PRELI domain-containing protein 1; PRELID 1; PRELID1; PRLD1_HUMAN; Protein of relevant evolutionary and lymphoid interest; PX 19; PX19; Px19 like protein; Px19, chicken, homolog of; Px19-like protein; SBBI12
Species
Homo sapiens (Human)
Expression Region
36-219
Target Protein Sequence
TEDIV HREVTPDQKL LSRRLLTKTN RMPRWAERLF PANVAHSVYV LEDSIVDPQN QTMTTFTWNI NHARLMVVEE RCVYCVNSDN SGWTEIRREA WVSSSLFGVS RAVQEFGLAR FKSNVTKTMK GFEYILAKLQ GEAPSKTLVE TAKEAKEKAK ETALAATEKA KDLASKAATK KQQQQQQFV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the modulation of the mitochondrial apoptotic pathway by ensuring the accumulation of cardiolipin (CL) in mitochondrial membranes. In vitro, the TRIAP1:PRELID1 complex mediates the transfer of phosphatidic acid (PA) between liposomes and probably functions as a PA transporter across the mitochondrion intermembrane space to provide PA for CL synthesis in the inner membrane. Regulates the mitochondrial apoptotic pathway in primary Th cells. Regulates Th cell differentiation by down-regulating STAT6 thereby reducing IL-4-induced Th2 cell number. May be important for the development of vital and immunocompetent organs.
Gene References into Functions
  1. This study suggests that the regulation of PRELID1 expression, by alternative polyadenylation and other mechanisms, plays a role in mitochondrial ROS signaling and represents a novel prognostic factor and therapeutic target in cancer PMID: 28912168
  2. PRELI plays an important role in the suppression of apoptosis in HepG2 cells in response to oxidative stress. PMID: 26275693
  3. Loss of TRIAP1 or PRELI impairs the accumulation of Cardiolipin, facilitates the release of cytochrome c, and renders cells vulnerable to apoptosis upon intrinsic and extrinsic stimulation. PMID: 23931759
  4. PRELI is a vital LEA B cell protein with failsafe genetics PMID: 22666421
  5. PRELI interacts with OPA1 to maintain mitochondria structures intact, sustain balanced ion(-)/proton(+) gradients, promote oxidative phosphorylation reactions, regulate pro- and antiapoptotic protein traffic and enable cell responses to induced death. PMID: 21364629

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Subcellular Location
Mitochondrion. Mitochondrion intermembrane space.
Tissue Specificity
Highly expressed in fetal liver; less expressed in fetal brain, lung, and kidney. At the adult stage, expression is drastically reduced in the liver but highly expressed in the spleen, brain, lung, lymph nodes and peripheral blood leukocytes.
Database Links

HGNC: 30255

OMIM: 605733

KEGG: hsa:27166

STRING: 9606.ENSP00000302114

UniGene: Hs.744904

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