Recombinant Human Pericentrin(PCNT) ,partial

Code CSB-YP017627HU
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Source Yeast
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Code CSB-EP017627HU
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Source E.coli
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Code CSB-EP017627HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017627HU
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Source Baculovirus
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Code CSB-MP017627HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names PCNT
Uniprot No. O95613
Alternative Names Centrosome Marker; Ken; Kendrin; KIAA0402; MOPD2; PCN; PCNT 2; PCNT; PCNT B; PCNT_HUMAN; PCNT1; PCNT2; PCNTB; PCTN2; Pericentrin 1; Pericentrin 2; Pericentrin 380; Pericentrin; Pericentrin B; Pericentrin-B; SCKL4
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
Gene References into Functions
  1. High pericentrin expression is associated with Disruptive Cilia Formation in down syndrome. PMID: 30100262
  2. we identified two novel mutations in the PCNT gene associated with Microcephalic osteodysplastic primordial dwarfism type II and intracranial aneurysms PMID: 26231886
  3. PCNT has to be phosphorylated by PLK1 to be a suitable substrate of separase. PMID: 26647647
  4. Cep68 degradation allows Cep215 removal from peripheral pericentriolar material (PCM) preventing centriole separation following disengagement, PCNT cleavage mediates Cep215 removal from core of the PCM to inhibit centriole disengagement and duplication PMID: 25503564
  5. The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. PMID: 24466316
  6. Data suggest that changes in the expression levels of PCNT in DS subjects may be involved into the molecular mechanism of Down's syndrome. PMID: 23979692
  7. PCNT gene mutation is associated with hematological abnormalities in patients with microcephalic osteodysplastic primordial dwarfism type II. PMID: 24106199
  8. Che-1 depletion abolishes the ability of Chk1 to bind pericentrin and to localize at centrosomes, which, in its turn, deregulates the activation of centrosomal cyclin B-Cdk1 and advances entry into mitosis. PMID: 23798705
  9. The pericentrin B cleavage is essential for timely centriole disengagement and duplication. PMID: 22722493
  10. Kendrin is a novel and crucial substrate for separase at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis. PMID: 22542101
  11. Expression of RRP1B, PCNT, KIF21A and ADRB2 in leucocytes of Down's syndrome subjects, was analyzed. PMID: 22552340
  12. The pericentrin (PCNT), a PCM protein, was specifically phosphorylated by PLK1 during mitosis. PMID: 22184200
  13. PCNT has a crucial role in tooth development; teeth of a patient with a novel homozygous mutation p.Glu1154X are probably the smallest ever reported. PMID: 21567919
  14. Severe insulin resistance and premature diabetes are common features of PCNT deficiency but are not congenital. PMID: 21270239
  15. Microcephalic osteodysplastic primordial dwarfism type II is a genetically homogeneous condition due to loss-of-function of pericentrin. PMID: 19643772
  16. Results found no significant association between the pericentrin gene and schizophrenia in the Japanese population. This gene may not play a major role independently in the etiology of SZ. PMID: 19937158
  17. The NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.(Pericentrin) PMID: 20567258
  18. Data suggest that pericentrin may regulate the intracellular distribution and secretion of insulin. PMID: 20676397
  19. CG-NAP and kendrin provide sites for microtubule nucleation in the mammalian centrosome by anchoring gamma-TuRC PMID: 12221128
  20. results confirm that MT1-MMP cleaves pericentrin-2 in humans but not in mice and that mouse models of cancer probably cannot be used to critically examine MT1-MMP functionality PMID: 16251193
  21. Elevated levels of PCNT2 might be implicated in the pathophysiology of bipolar disorder. PMID: 17884020
  22. Mutations in PCNT cause Seckel syndrome with defective ATR-dependent DNA damage signaling. PMID: 18157127
  23. study found that biallelic loss-of-function mutations in the centrosomal pericentrin (PCNT) gene on chromosome 21q22.3 cause microcephalic osteodysplastic primordial dwarfism type II (MOPD II) in 25 patients PMID: 18174396
  24. Upregulation of pericentrin is associated with increased angiogenesis and prostate tumor cell proliferation PMID: 18701509
  25. These findings suggest that the DISC1-kendrin interaction plays a key role in the microtubule dynamics. PMID: 18955030
  26. The association of PCNT2 and DBZ with schizophrenia and bipolar disorder in a case-control study of Japanese cohorts, was examined. PMID: 19191256
  27. results suggest that genetic variations in the PCNT gene may play a significant role in the etiology of major depressive disorder in the Japanese population PMID: 19448849
  28. Results show that a lack of microcephalin or pericentrin results in a loss of Chk1 from centrosomes with subsequently deregulated activation of centrosomal cyclin B-Cdk1. PMID: 19546241

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Involvement in disease Microcephalic osteodysplastic primordial dwarfism 2 (MOPD2)
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Tissue Specificity Expressed in all tissues tested, including placenta, liver, kidney and thymus.
Database Links

HGNC: 16068

OMIM: 210720

KEGG: hsa:5116

STRING: 9606.ENSP00000352572

UniGene: Hs.474069

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