Recombinant Human Peroxiredoxin-5, mitochondrial (PRDX5)

Code CSB-YP018658HU
MSDS
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Source Yeast
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Code CSB-EP018658HU
MSDS
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Source E.coli
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Code CSB-EP018658HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018658HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP018658HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRDX5
Uniprot No.
Alternative Names
ACR1; Alu co repressor 1; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; B166; epididymis secretory protein Li 55; HEL-S-55; Liver tissue 2D-page spot 71B; mitochondrial; Peroxiredoxin 5; mitochondrial; Peroxiredoxin V; Peroxiredoxin-5; Peroxisomal antioxidant enzyme; PLP; PMP20; PRDX 5; PRDX5; PRDX5_HUMAN; PRDX6; Prx-V; PRXV; SBBI10; Thioredoxin peroxidase PMP20; Thioredoxin reductase; TPx type VI
Species
Homo sapiens (Human)
Expression Region
53-214
Target Protein Sequence
MAPIKVGD AIPAVEVFEG EPGNKVNLAE LFKGKKGVLF GVPGAFTPGC SKTHLPGFVE QAEALKAKGV QVVACLSVND AFVTGEWGRA HKAEGKVRLL ADPTGAFGKE TDLLLDDSLV SIFGNRRLKR FSMVVQDGIV KALNVEPDGT GLTCSLAPNI ISQL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
Gene References into Functions
  1. High PRX5 expression is associated with gastric cancer. PMID: 28535004
  2. Results found that Prx5 was increased in the cellular model of Alzheimer's disease (AD) and, identify a role for Prx5 as an upregulator of Cdk5 activation via reactive oxygen species-mediated Ca2+-mediated calpain activation. PMID: 28358580
  3. The PRX5 can be used as a predictive biomarker and serves as a putative therapeutic target for the development of clinical treatments for human CRC. PMID: 28431931
  4. peroxisomal PRDX5 protects oligodendrocytes against peroxisomal and mitochondrial oxidative stress. PMID: 25772011
  5. show here how the ligand-observed saturation transfer difference (STD) experiment and the PRDX5 protein-observed 15N-HSQC experiment, two popular NMR screening experiments, can be used to compare the binding modes of analogous fragments PMID: 25025339
  6. PRX5 is either consumed or its production is impaired in severe stroke PMID: 24385276
  7. This study demonistrated that overexpression of mitochondrial PRDX5 protects SH-SY5Y cells against the ROS/RNS-dependent neuronal death induced by MPP+. PMID: 23216451
  8. The promoter region critical for Prx5 gene regulation to which the novel negative transcription regulator, GATA1 binds in human breast cancer cell lines. PMID: 22020876
  9. basal expression of the human PRDX5 gene is regulated by GABP PMID: 20937353
  10. analysis of fragment molecules that bind the human peroxiredoxin 5 active site PMID: 20305821
  11. Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanism PMID: 11832487
  12. the expression of peroxiredoxin 5 is upregulated in osteoarthritis PMID: 12417342
  13. multiple subcellular targeting of peroxiredoxin 5 in mammalian cells can be implicated in antioxidant protective mechanisms under nonpathological conditions PMID: 14732291
  14. Transfection of PRXVI protects immortalized human WI-38 and murine NIH3T3 fibroblasts against cytotoxic doses of tert-butylhydroperoxide and H2O2 PMID: 14741336
  15. new crystal form of human peroxiredoxin 5 is described at 2.0 A resolution; under initial reducing conditions, one chain remains oxidized and forms an homodimer with a symmetry-related one through intermolecular disulfide bonds between Cys47 and Cys151 PMID: 15046979
  16. plays a protective role in human tendon cells against oxidative stress by reducing apoptosis and maintaining collagen synthesis PMID: 15276323
  17. Human peroxiredoxin 5 is also a peroxynitrite reductase. PMID: 15280035
  18. nuclear PrxV associates with coilin-containing bodies suggesting possible interaction of this protein with transcription complexes PMID: 15304327
  19. The level of expression is directly correlated with the functional status of epithelial cells, being higher in multinodular goiters, and even more pronounced in hyperthyroid tissues, such as Graves' disease. PMID: 15785239
  20. mitochondrial peroxiredoxin 5 may play an important role in mitochondrial genome stability PMID: 15848167
  21. these findings suggest that PrxV is a multifunctional protein, which is essential for protection against apoptosis induced by anticancer drugs. PMID: 16781710
  22. Together, these results suggest that constitutively expressed PRDX5 gene plays an important role in protecting the genome against oxidation and may also be involved in the control of transcription of noncoding DNA. PMID: 16817890
  23. expression in white matter from normal human brain and MS patients PMID: 17623739
  24. we present new alternative splicing variants encoded specifically by human PRDX5 gene. The characterization of human PRDX5 gene revealed complexity of its regulation & high variability of sequences that might be associated with pathological situations. PMID: 17628720
  25. kinetic analysis of human peroxiredoxin 5 PMID: 17892856
  26. Peroxiredoxin V (PRXV) is an important antioxidant protein of lung epithelial cells. Its expression in the human lung increases in inflammation. PMID: 18219526
  27. report here three crystal forms in which this intramolecular disulfide bond is indeed observed. The structures are characterized by the expected local unfolding of the peroxidatic loop, but also by the unfolding of the resolving loop PMID: 18489898
  28. Ets regulates PRDX5 expression through their interaction with HGMB1 protein. PMID: 19016754
  29. crystal structure at 1.5 angstrom resolution PMID: 11518528

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Subcellular Location
[Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic+peroxisomal]: Cytoplasm. Peroxisome matrix.
Protein Families
Peroxiredoxin family, Prx5 subfamily
Tissue Specificity
Widely expressed.
Database Links

HGNC: 9355

OMIM: 606583

KEGG: hsa:25824

STRING: 9606.ENSP00000265462

UniGene: Hs.502823

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