Product Details

Uniprot No.

Target Names

PRDX5

Alternative Names

ACR1 antibody; Alu co repressor 1 antibody; Alu corepressor 1 antibody; Antioxidant enzyme B166 antibody; AOEB166 antibody; B166 antibody; epididymis secretory protein Li 55 antibody; HEL-S-55 antibody; Liver tissue 2D-page spot 71B antibody; mitochondrial antibody; Peroxiredoxin 5; mitochondrial antibody; Peroxiredoxin V antibody; Peroxiredoxin-5 antibody; Peroxisomal antioxidant enzyme antibody; PLP antibody; PMP20 antibody; PRDX 5 antibody; PRDX5 antibody; PRDX5_HUMAN antibody; PRDX6 antibody; Prx-V antibody; PRXV antibody; SBBI10 antibody; Thioredoxin peroxidase PMP20 antibody; Thioredoxin reductase antibody; TPx type VI antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PRDX5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Peroxiredoxin 5 serves as a critical antioxidant enzyme with broad subcellular distribution, functioning in mitochondria, peroxisomes, and the cytosol to protect cells against oxidative stress. This thioredoxin peroxidase plays essential roles in cellular redox homeostasis and has been implicated in various pathological conditions where oxidative damage contributes to disease progression, making it a valuable target for researchers investigating cellular stress responses, metabolic regulation, and tissue-specific antioxidant mechanisms.

This recombinant monoclonal antibody against human PRDX5 offers the reproducibility and consistency that demanding experimental workflows require. Generated from a defined clone and produced using recombinant technology, each lot delivers identical binding characteristics, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target while minimizing background interference.

Validation across multiple platforms demonstrates this antibody's versatility in your research applications. Immunohistochemistry testing in paraffin-embedded human kidney tissue at dilutions of 1:50 to 1:200 reveals clear target detection using standard citrate buffer antigen retrieval protocols on automated staining systems. Flow cytometry analysis in HeLa cells confirms reliable intracellular staining with distinct signal separation from isotype controls, supporting quantitative studies of PRDX5 expression at the single-cell level. The antibody is also validated for ELISA applications, providing additional flexibility for high-throughput screening approaches.

Whether you are investigating mitochondrial dysfunction, characterizing tissue-specific antioxidant responses, or exploring PRDX5's emerging roles as a transcriptional corepressor, this antibody provides a dependable tool for advancing your cell biology research with confidence in your results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.

Gene References into Functions

High PRX5 expression is associated with gastric cancer. PMID: 28535004
Results found that Prx5 was increased in the cellular model of Alzheimer's disease (AD) and, identify a role for Prx5 as an upregulator of Cdk5 activation via reactive oxygen species-mediated Ca2+-mediated calpain activation. PMID: 28358580
The PRX5 can be used as a predictive biomarker and serves as a putative therapeutic target for the development of clinical treatments for human CRC. PMID: 28431931
peroxisomal PRDX5 protects oligodendrocytes against peroxisomal and mitochondrial oxidative stress. PMID: 25772011
show here how the ligand-observed saturation transfer difference (STD) experiment and the PRDX5 protein-observed 15N-HSQC experiment, two popular NMR screening experiments, can be used to compare the binding modes of analogous fragments PMID: 25025339
PRX5 is either consumed or its production is impaired in severe stroke PMID: 24385276
This study demonistrated that overexpression of mitochondrial PRDX5 protects SH-SY5Y cells against the ROS/RNS-dependent neuronal death induced by MPP+. PMID: 23216451
The promoter region critical for Prx5 gene regulation to which the novel negative transcription regulator, GATA1 binds in human breast cancer cell lines. PMID: 22020876
basal expression of the human PRDX5 gene is regulated by GABP PMID: 20937353
analysis of fragment molecules that bind the human peroxiredoxin 5 active site PMID: 20305821
Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanism PMID: 11832487
the expression of peroxiredoxin 5 is upregulated in osteoarthritis PMID: 12417342
multiple subcellular targeting of peroxiredoxin 5 in mammalian cells can be implicated in antioxidant protective mechanisms under nonpathological conditions PMID: 14732291
Transfection of PRXVI protects immortalized human WI-38 and murine NIH3T3 fibroblasts against cytotoxic doses of tert-butylhydroperoxide and H2O2 PMID: 14741336
new crystal form of human peroxiredoxin 5 is described at 2.0 A resolution; under initial reducing conditions, one chain remains oxidized and forms an homodimer with a symmetry-related one through intermolecular disulfide bonds between Cys47 and Cys151 PMID: 15046979
plays a protective role in human tendon cells against oxidative stress by reducing apoptosis and maintaining collagen synthesis PMID: 15276323
Human peroxiredoxin 5 is also a peroxynitrite reductase. PMID: 15280035
nuclear PrxV associates with coilin-containing bodies suggesting possible interaction of this protein with transcription complexes PMID: 15304327
The level of expression is directly correlated with the functional status of epithelial cells, being higher in multinodular goiters, and even more pronounced in hyperthyroid tissues, such as Graves' disease. PMID: 15785239
mitochondrial peroxiredoxin 5 may play an important role in mitochondrial genome stability PMID: 15848167
these findings suggest that PrxV is a multifunctional protein, which is essential for protection against apoptosis induced by anticancer drugs. PMID: 16781710
Together, these results suggest that constitutively expressed PRDX5 gene plays an important role in protecting the genome against oxidation and may also be involved in the control of transcription of noncoding DNA. PMID: 16817890
expression in white matter from normal human brain and MS patients PMID: 17623739
we present new alternative splicing variants encoded specifically by human PRDX5 gene. The characterization of human PRDX5 gene revealed complexity of its regulation & high variability of sequences that might be associated with pathological situations. PMID: 17628720
kinetic analysis of human peroxiredoxin 5 PMID: 17892856
Peroxiredoxin V (PRXV) is an important antioxidant protein of lung epithelial cells. Its expression in the human lung increases in inflammation. PMID: 18219526
report here three crystal forms in which this intramolecular disulfide bond is indeed observed. The structures are characterized by the expected local unfolding of the peroxidatic loop, but also by the unfolding of the resolving loop PMID: 18489898
Ets regulates PRDX5 expression through their interaction with HGMB1 protein. PMID: 19016754
crystal structure at 1.5 angstrom resolution PMID: 11518528

Hide All

Subcellular Location

[Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic+peroxisomal]: Cytoplasm. Peroxisome matrix.

Protein Families

Peroxiredoxin family, Prx5 subfamily

Tissue Specificity

Widely expressed.

Database Links

HGNC: 9355

OMIM: 606583

KEGG: hsa:25824

STRING: 9606.ENSP00000265462

UniGene: Hs.502823

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Code	CSB-RA161234A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA161234A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA161234A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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PRDX5 Recombinant Monoclonal Antibody

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Target Background