Recombinant Human Phenylethanolamine N-methyltransferase (PNMT)

Code CSB-YP018274HU
MSDS
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Source Yeast
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Code CSB-EP018274HU
MSDS
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Source E.coli
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Code CSB-EP018274HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018274HU
MSDS
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Source Baculovirus
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Code CSB-MP018274HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PNMT
Uniprot No.
Alternative Names
Noradrenaline N-methyltransferase; PENT; Phenylethanolamine N-methyltransferase; PNMT; PNMT_HUMAN; PNMTase
Species
Homo sapiens (Human)
Expression Region
1-282
Target Protein Sequence
MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Converts noradrenaline to adrenaline.
Gene References into Functions
  1. PNMT underexpression is associated with malignant pheochromocytoma and paraganglioma. PMID: 27007161
  2. initial velocity studies on human PNMT in the absence and presence of product and dead end inhibitors. PMID: 24018397
  3. The 2-SNP AA haplotype in the PNMT promoter is associated with decreased risk of essential hypertension in Han Chinese. PMID: 21866188
  4. PNMT G-390A polymorphism is possibly associated with essential hypertension risk in male Chinese Han population. PMID: 19952402
  5. In Caucasians, phenylethanolamine N-methyltransferase single nucleotide polymorphisms are associated with the development of acute kidney injury, disease severity, and in-hospital mortality. PMID: 20090367
  6. PNMT variants in cis may interact with nuclear factors in trans to govern adrenergic activity. PMID: 20204374
  7. Human PNMT has been crystallized in complex with an inhibitor and the cofactor product S-adenosyl-L-homocysteine and diffraction data measured from a cryocooled crystal extend to a resolution of 2.3 A. PMID: 11807261
  8. pharmacologic and biochemical evidence of beta-carboline 2N-methyltransferase activity PMID: 11900856
  9. Association between the phenylethanolamine N-methyltransferase gene and multiple sclerosis. PMID: 11958827
  10. homozygosity/heterozygosity of the phenylethanolamine N-methyltransferase gene polymorphism was highly predictive of significant weight loss with sibutramine during the first 3 months of obesity treatment PMID: 12634439
  11. genetic variants of PNMT may play a role in the development of essential hypertension. PMID: 14553966
  12. Presence of local PNMT transcription in human heart after transplantation. PMID: 15848714
  13. disulfide-linked dimers are as active as the monomeric enzyme indicating that the crystal structure of the protein is a valid target for inhibitor design. PMID: 15893506
  14. Nicotinic stimuli appear to regulate expression of the epinephrine-synthesizing gene PNMT through a previously uncharacterized regulatory element. PMID: 15968085
  15. Results suggest that PNMT catalyzes transfer of methyl to ligand amines only when "anchor" interactions, such as those identified for the beta-hydroxyls of p-octopamine and cis-AT, are present. PMID: 16363801
  16. the differences in PNMT expression between normotensives and hypertensives are not determined by the polymorphisms in this gene, but rather by the interplay of gene expression regulators, which may vary among individuals PMID: 17645789
  17. We examined the correlation between reward dependence (RD) trait, and 5 polymorphisms in genes of norepinephrine pathways. We found that rs3764351 in PNMT was significantly associated with reward dependence trait. PMID: 18702937
  18. Haplotypes of the phenylethanolamine N-methyltransferase (PNMT), catechol-O-methyltransferase (COMT) have functionally important effects on alcohol-induced cardiovascular symptoms by affecting blood catecholamine levels. PMID: 18715275
  19. The crystal structures illustrate the adaptability of the PNMT substrate binding site in accepting multi-fused ring systems, such as substituted norbornene, as well as noradrenochrome, the oxidation product of noradrenaline. PMID: 19570037
  20. the reaction takes place via an SN2 mechanism with methyl transfer being rate-limiting PMID: 19733262
  21. Oncogenomic recombination hotspot around the PPP1R1B-STARD3-TCAP-PNMT-PERLD1-ERBB2-C17orf37-GRB7 amplicon at human chromosome 17q12 is closely linked to evolutionary recombination hotspot around the GSDML-GSDM locus. PMID: 15010812

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Protein Families
Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family
Database Links

HGNC: 9160

OMIM: 171190

KEGG: hsa:5409

STRING: 9606.ENSP00000269582

UniGene: Hs.1892

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