Recombinant Human Phospholipid scramblase 1(PLSCR1)

Code CSB-EP018207HU
Size US$1726
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names PLSCR1
Uniprot No. O15162
Research Area Microbiology
Alternative Names Ca(2+) dependent phospholipid scramblase 1; Ca(2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MM1 cell-derived transplantability-associated gene 1b; mouse; homolog of; MmTRA1a; MmTRA1b; Nor1; Phospholipid scramblase 1; PL scramblase 1; PLS1_HUMAN; PLSCR 1; Plscr1; Scramblase1; Tra1; Tra1a; Tra1b; Transplantability-associated protein 1; Tras1; Tras2
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-318aa
Target Protein Sequence ESTGSQEQKSGVW
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 28.4kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.; FUNCTION
Gene References into Functions
  1. Inhibition of PLSCR1 decreased the antiviral activity of IFN against HBV. PMID: 30004207
  2. Data suggest that PLSCR1 is involved in viral entry; here, herpes simplex viruses activate PLSCR1 and up-regulate flipping of phosphatidylserines and AKT to outside of plasma membrane shortly following HSV-1 or HSV-2 exposure. (PLSCR1 = phospholipid scramblase 1; AKT = proto-oncogene c-Akt) PMID: 29293671
  3. wogonoside promotes the expression of PLSCR1 and enhances its nuclear translocation and binding to the 1, 4, 5-trisphosphate receptor 1 (IP3R1) promoter in AML patient-derived primary cells. Wogonoside activates IP3R1, in turn, promotes release of Ca(2+) from endoplasmic reticulum, and eventually leads to cell differentiation. PMID: 28492556
  4. Data indicate heterogeneous expression of phospholipid scramblase 1 (PLSCR1) and suggest its possible implication in the response to anticancer therapies, especially to drugs promoting protective autophagy. PMID: 27248824
  5. investigate how the polar aspartate residue is accommodated in lipid bilayers containing POPC with and without cholesterol, using all-atom molecular dynamics simulations PMID: 28372945
  6. Human phospholipid scramblase 1 has five histidine residues and point mutations of histidine residues to alanine in hPLSCR1 resulted in 60 % loss in nuclease activity. PMID: 27206388
  7. The mechanisms of pH-induced functional activation of hPLSCR1 are described. PMID: 26204401
  8. PLSCR1 positively regulates hepatic carcinoma cell proliferation and migration through interacting with midkine PMID: 26642712
  9. Suggest role for PLSCR1 in negatively regulating trophoblast fusion rather than directly promoting fusion. PMID: 25362260
  10. PLSCR1 mediates the antiviral activity and anticarcinogenesis against hepatitis B virus by regulating HBx stability. PMID: 25365352
  11. affinity of SCR for cholesterol-rich domains in membranes PMID: 25229146
  12. This is the first biochemical evidence to prove the above hypothesis that hPLSCR1 is activated in heavy metal poisoning, which leads to bidirectional transbilayer movement of phospholipids. PMID: 23659204
  13. The C-terminal transmembrane domain of human phospholipid scramblase 1 is essential for the protein flip-flop activity and Ca(2)-binding. PMID: 24343571
  14. This is the first report showing the transcriptional regulation of hPLSCR1 expression by Snail TF and its possible implications in cancer progression. PMID: 24878522
  15. PLSCR1 interacted with ANG in the cell nucleus and regulated rRNA transcription. PMID: 24356419
  16. hPLSCR1 and PRD-hPLSCR2 showed Ca(2+)-dependent aggregation and scrambling activity PMID: 24648509
  17. The data are in agreement with the possibility that PLSCR1 is an integral membrane protein. PMID: 24099740
  18. Phospholipid Scramblase 1, an interferon-regulated gene located at 3q23, is regulated by SnoN/SkiL in ovarian cancer cells. PMID: 23621864
  19. The C-terminal helix of human PLSCR1 is required for membrane insertion and calcium binding. PMID: 23590222
  20. Phospholipid scramblase 1 expression is enhanced in patients with antiphospholipid syndrome. PMID: 22526829
  21. Overexpression of PLSCR1 efficiently represses the Tat-dependent transactivation of the HIV-1 long terminal repeat (LTR) and reduces the nuclear translocation of Tat. PMID: 23501106
  22. Calcium-dependent PLSCR-1 activity is required for compensatory endocytosis (but not for exocytosis) in neuroendocrine cells. PMID: 23426682
  23. PLSCR1 is overexpressed in colorectal cancer and metastatic liver cancer. Silencing of PLSCR1 by siRNA inhibits the proliferation, adhesion, migration and invasion of tumor cells. PMID: 22893466
  24. PLSCR1 may play an important role in the IFN-mediated repression of Tax-dependent transactivation during HTLV-1 infection. PMID: 22789739
  25. this report identifies PLSCR1 as a protein that physically interacts with Receptor expressed in lymphoid tissues family members and is a potential substrate for phosphorylation by the OSR1 kinase in a RELT-dependent fashion. PMID: 22052202
  26. PLSCR1 inhibited HBV replication through a reduction in the synthesis of viral proteins and DNA replication. PMID: 22342889
  27. IFNalpha-induced PLSCR1 associates with the cytoskeleton after exposure to alpha-toxin, and cellular depletion of PLSCR1 negates IFN-induced protection from alpha-toxin. PMID: 22264514
  28. summarizes the mechanisms and roles of PS exposure following platelet activation and discusses the recent identification of TMEM16F and its significance in the scrambling process PMID: 21958383
  29. PLSCR1 might be used as a noninvasive serological diagnostic and prognostic biomarker for CRC. PMID: 20927484
  30. PLSCR1 was required for the initial attachment of HCV onto hepatoma cells, where it specifically interacted with entry factor OCLN. We show that PLSCR1 is a novel attachment factor for HCV entry. PMID: 21806988
  31. Interferon tau induces expression of DDX58 and PLSCR1 via the classical STAT1-mediated cell signaling pathway. PMID: 20926691
  32. PLSCR1 interacts with the tandem repeat region of ECM1a in the dermal epidermal junction zone of human skin. PMID: 20870722
  33. Monocytes in systemic lupus erythematosus patients had enhanced PLSCR1 mRNA expression, as well as increased fibrin turnover and cell-surface PS exposure. PMID: 20516018
  34. Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. PMID: 11809687
  35. PLSCR1 co-localizes with the epidermal growth factor (EGF) receptor in lipid rafts and plays a role in the EGF-induced metabolic or mitogenic response. PMID: 12009895
  36. expression has a role in the induction of differentiation of human myeloid leukemia cells into granulocytes. PMID: 12031648
  37. Trafficking of PLSCR1 to the plasma membrane is dependent upon palmitoylation of the polypeptide; in the absence of palmitoylation, PLSCR1 traffics into the nucleus. PMID: 12564925
  38. Identification of domains for PLSCR1 and BACE binding and their colocalization in the Golgi area and endosomal compartments suggest involvement of PLSCR1 in the intracellular distribution and/or recruitment of BACE into the lipid raft. PMID: 12586838
  39. phospholipid scramblase 1 plays a role in ischemic injury in the human hippocampus PMID: 12605885
  40. PLSCR1, through its interaction with Shc, promotes Src kinase activation through the EGF receptor. PMID: 12871937
  41. Higher MmTRA1b mRNA levels were associated with significantly longer overall survival in AML, especially in AML-M4 patients, independent of chromosomal aberrations PMID: 14654079
  42. both PLSCR1 and phospholipid flip-flop characterize uropod raft domains of polarized neutrophils PMID: 14766753
  43. Data support a mechanism of receptor-mediated nuclear import of PLSCR1 and suggest a potential nuclear function for this plasma membrane protein, mediated through potential interaction with genomic DNA. PMID: 15035622
  44. as a protein induced upon PKCdelta activation, PLSCR1 is required for ATRA- and PMA-triggered leukemic cell differentiation PMID: 15308560
  45. PLSCR1, which is itself an IFN-stimulated gene-encoded protein, provides a mechanism for amplifying and enhancing the IFN response through increased expression of a select subset of potent antiviral genes PMID: 15308695
  46. x-ray crystallography shows that there is a nonclassical nuclear localization signal in PLSCR1 with a unique binding site in importin alpha PMID: 15611084
  47. PLSCR1 binds to the promoter region of the IP3R1 gene to enhance its expression PMID: 16091359
  48. protein kinase Cdelta and JNK have roles in Ifn-alpha induced expression of phospholipid scramblase 1 through STAT1 PMID: 16260419
  49. PR3 externalization depended on PLSCR1 PMID: 17712045
  50. the same region of the cytoplasmic domain of PLSCR1 is involved in the binding to CD4 and SLPI PMID: 19333378
  51. Results show that binding affinities of the peptides are in the order hPLSCR1>hPLSCR3>hPLSCR2>hPLSCR4 for Ca2+ and in the order hPLSCR1>hPLSCR2>hPLSCR3>hPLSCR4 for Mg2+. PMID: 19540310
  52. Paper reports studies on PLSCR1 gene knockout mice. PLSCR1-/- mice displayed no hemostatic abnormality. By contrast a defect in hematopoiesis was identified and shown to reflect defective response of hematopoietic precursors to select growth factors. PMID: 12010804

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Subcellular Location Cell membrane, Single-pass type II membrane protein, Membrane, Lipid-anchor, Cytoplasmic side, Nucleus
Protein Families Phospholipid scramblase family
Tissue Specificity Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
Database Links

HGNC: 9092

OMIM: 604170

KEGG: hsa:5359

STRING: 9606.ENSP00000345494

UniGene: Hs.130759

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