Recombinant Human Phospholipid transfer protein(PLTP)

Code CSB-EP018212HU
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names PLTP
Uniprot No. P55058
Research Area Transport
Alternative Names BPI fold containing family E; BPIFE; HDLCQ9; High density lipoprotein cholesterol level quantitative trait locus 9; included; Lipid transfer protein II; OD107; Phospholipid transfer protein; Plasma phospholipid transfer protein ; Pltp; PLTP_HUMAN
Species Homo sapiens (Human)
Source E.coli
Expression Region 18-493aa
Target Protein Sequence EFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGHFYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINASAEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 80.1kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma
Gene References into Functions
  1. Study demonstrated that PLTP is overexpressed in synovial tissue of patients with chronic inflammatory rheumatisms, such as rheumatoid arthritis (RA), when compared to osteoarthritis patients (OA); and that RA but not OA patients displayed elevated levels of PLTP activity in synovial fluid, which were correlated with pro-inflammatory cytokine levels. PMID: 29565987
  2. These results indicate that PLTP and MASP-1 can serve as plasma biomarkers for the early diagnosis and treatment of Age-related macular degeneration , which is critical for preventing Age-related macular degeneration -related blindness. PMID: 27605007
  3. PLTP activity did not affect this metric. Thus, deviations in PLTP activity from the wild-type level reduce HDL mass and ex vivo cholesterol efflux capacity, but not the rate of macrophage cholesterol efflux to plasma or in vivo mRCT. PMID: 28137768
  4. serum CETP and PLTP activity in patients diagnosed with hypothyroidism, was examined. PMID: 27899788
  5. PLTP may regulate cigarette smoke extract-induced IL-8 expression via the ERK1/2 signaling pathway in human pulmonary epithelial cells PMID: 27596005
  6. LPL and PLTP appear to be novel glioma-associated proteins and play a role in the progression of human glioma PMID: 27864281
  7. association of PON-1 activity with PLTP activity was lost in analysis which included large HDL particles PMID: 26656640
  8. a novel association between PLTPa and PON1 activity PMID: 26009633
  9. The domain of apolipoprotein E4 required for PLTP activation resides within its amino-terminal 1-185 region. PMID: 26337529
  10. Elevated baseline plasma levels of PLTP are associated with an increased risk of long-term all-cause mortality in patients with diabetes and known or suspected coronary disease. PMID: 25710294
  11. This review summarizes the recent progresses made in the PLTP research field and focuses on the complexity of the implication of PLTP in obesity, insulin resistance and type 2 diabetes mellitus. [review] PMID: 25107452
  12. Elevated plasma PLTP activity may predict an increased risk of T2DM in men PMID: 25304745
  13. High systemic PLTP expression does not contribute significantly to a renal phenotype despite being implicated in systemic atherosclerosis in ApoE deficient mice. PMID: 25651711
  14. proteolytic cleavage of PLTP by cathepsin G may enhance the injurious inflammatory responses that occur in COPD PMID: 24532668
  15. PLTP is actively involved in lipid transfer, cholesterol efflux, HDL genesis, and remodeling at the blood-brain barrier. PMID: 24369175
  16. Lower plasma CETP or higher PLTP activity was each associated with a significantly increased risk of cardiovascular disease. PMID: 23477743
  17. Higher PLTP activity is associated with depressed LV systolic function in a dose-dependent manner. PMID: 23545183
  18. In all the models with ApoE deficiency, elevated human PLTP expression causes higher levels of diet-induced atherosclerosis coinciding with decreased plasma levels of HDL cholesterol. PMID: 23313246
  19. The physiological relevance of PLTP in intracellular lipid metabolism and signal transduction. [Review] PMID: 21736953
  20. PLTP mediates PL transfer and participates in reverse cholesterol transport pathways at the fetoplacental barrier. PMID: 22492872
  21. we provide evidences that induction of TGF-B1 production and Smad3 phosphorylation by Ox-LDL is mediated by Ras/ERK/PLTP pathway in human alveolar epithelial cells. PMID: 22034170
  22. diabetes-environment and diabetes-gene interactions govern plasma PLTP activity PMID: 21973210
  23. Pltp expression is increased in GDM with hyperglycemia and/or hyperinsulinemia contributing. High PLTP activity in fetal serum may enhance conversion of HDL into cholesterol-accepting particles, thereby increasing maternal-fetal cholesterol transfer. PMID: 22090281
  24. PLTP activity could contribute to elevated cardiovascular risk in the presence of obesity and insulin resistance PMID: 21481395
  25. PLTP has anti-inflammatory capabilities in macrophages. PMID: 21782857
  26. Increased PLTP activity in human tear fluid in dry eye syndrome patients suggests an ocular surface protective role for this lipid transfer protein. PMID: 21514421
  27. Data show that the removal of the carbohydrate chains had a quantitative impact on cellular secretion of PLTP and its phospholipid transfer activity. PMID: 21515415
  28. data show the existence of small inactive plasma PLTP complexes, and show that variation in the amount of the two clusters of inactive PLTP complexes and specific activity of active PLTP contribute to the variation in plasma PLTP specific activity. PMID: 21303701
  29. the SR-BI pathway contributes in unique ways to cholesterol metabolism and atherosclerosis susceptibility even in the presence of CETP PMID: 21454568
  30. Human PLTP expression in transgenic rabbits worsens atherosclerosis as a result of increased levels of atherogenic apoB-containing lipoproteins. PMID: 21252068
  31. CETP and PLTP activities were negatively related with the ratio of HDL-C/LDL-C. PMID: 20937151
  32. a dual role for PLTP in human tear fluid PMID: 20724654
  33. Our results indicate that there is no genetic association between PLTP and Alzheimer's disease PMID: 20714154
  34. PLTP was overexpressed in the ER-negative breast cancer group. PMID: 20805453
  35. Data show that biglycan (BGN) induces of phospholipid transfer protein (PLTP) in aortic valve interstitial cells via stimulation of Toll-like receptor 2, so increased BGN in stenotic valves contributes to the production of PLTP via TLR 2. PMID: 20382708
  36. A gene score based on 2 PLTP single nucleotide polymorphisms is associated with lower PLTP transcription and activity, an increased number of HDL particles, smaller HDL size, and decreased risk of cardiovascular disease PMID: 20644014
  37. apoA-I enhances the phospholipid transfer activity of PLTP secreted from macrophage foam cells without affecting the PLTP mass. PMID: 20534134
  38. PLTP was found to mediate transfer of anionic phospholipids to HDL/LDL lipoproteins thereby neutralizing the effect of procoagulant liposomes, resulting in a reduction of procoagulant activity. PMID: 20088939
  39. PLTP activity is strongly influenced by PLTP region polymorphisms and metabolic factors. PMID: 19965587
  40. serum PLTP is involved in the clearance of postprandial lipoproteins and this process is attenuated in diabetes. PMID: 20108050
  41. PLTP activity may represent a novel marker of left ventricular systolic dysfunction in patients with known or suspected coronary artery disease. PMID: 19446293
  42. Human PLTP expressed in plasma from transgenic mice contributes to the formation of pre beta-HDL, whereas the expression of human cholesterol ester transfer protein does not affect this process. PMID: 11716766
  43. This protein and pre-beta-high density lipoprotein are degraded by mast cell chymase, the high affinity efflux of cholesterol from macrophage foam cells is impaired. PMID: 12531890
  44. The phospholipid transfer protein gene is a liver X receptor target expressed by macrophages in atherosclerotic lesions. PMID: 12612088
  45. Elevation of PLTP activity results in rapid disposal of cholesterol from the body via increased conversion into bile acids and subsequent excretion. PMID: 12649089
  46. PLTP is expressed by macrophages, is regulated by cholesterol loading, and is present in atherosclerotic lesions PMID: 12730304
  47. elevated PLTP activity in hypertriglyceridemia is related to insulin resistance and not to increased PLTP mass PMID: 12754275
  48. HepG2-derived PLTP resembles in several aspects the high-activity form of PLTP found in human plasma PMID: 12810820
  49. PLTP is present in human atherosclerotic lesions, and its distribution suggests roles for PLTP in both cellular cholesterol metabolism and lipoprotein retention on extracellular matrix PMID: 12835223
  50. seminal PLTP concentrations represented 25% of the concentration measured in blood plasma; seminal PLTP activity was partially associated with prostasomes; higher PLTP activity was measured in seminal plasma samples with low seminal vesicle secretions PMID: 12837922
  51. PLTP concentration serve as a predictor of Coronary disease, independent of HDL cholesterol, triglycerides and other established risk factors. PMID: 14695459
  52. expression of PLTP results in increased atherosclerosis in spite of reduced apoB-containing lipoproteins, by reduction of HDL and of HDL-associated antioxidant enzyme activities PMID: 14993244
  53. Phospholipid transfer protein is emerging as a multifaceted and multifunctional player in lipid and lipoprotein metabolism [review]. PMID: 15166780
  54. patthophysiologic role of PLTP in decreasing the vitamin E content of apolipoprotein B-containing lipoproteins in Type 2 diabetes. PMID: 15448094
  55. A major role for stimulated phospholipid transfer protein (PLTP) activity is identified in the recycling of pre-beta 1-high density lipoprotein during reverse cholesterol transport. PMID: 15544352
  56. The findings of reduced PLTP activity in AD CSF, and the observation that PLTP can influence apoE secretion in astrocytes suggest a potential link between alterations in the brain lipid metabolism and AD pathogenesis. PMID: 15795933
  57. ApoE proteoliposomes induced a concentration-dependent activation of low-activity form of PLTP. PMID: 15845396
  58. Human tear fluid contains catalytically active phospholipid transfer protein (PLTP), which resembles the active form of PLTP present in plasma. PMID: 15924430
  59. These findings indicate that PLTP gene variation is an important determinant of plasma lipoproteins and affects disorders of HDL metabolism. PMID: 16388083
  60. The increase in PLTP activity in type 2 diabetes is partly related to the changes in apolipoprotein E. PMID: 16389649
  61. Active plasma phospholipid transfer protein is associated with apoA-I- but not apoE-containing lipoproteins. PMID: 16520487
  62. Association between the decrease in PLTP activity and aapolipoprotein E during statin treatment supports the hypothesis that apoE may prevent PLTP inactivation in diabetes. PMID: 16644710
  63. results suggest that secretion-competent PLTP requires glycosylation but that no single glycosylation site is required PMID: 16862459
  64. PLTP is capable of transferring lipids by a shuttle mechanism and formation of a ternary complex between PLTP, acceptor, and donor particles is not necessary for phospholipid transfer PMID: 17260960
  65. Lipolysis of triglyceride-rich lipoproteins and their remodelling by PLTP interact to disturb cellular phospholipid flux and intracellular signaling processes, ultimately leading to apoptosis in human macrophages and endothelial cells. PMID: 17272752
  66. We resequenced candidate genes for HDL regulation and identified several functional nonsynonymous mutations including 1 in phospholipid transfer protein (PLTP), leaving 88% (110/124) of HDL deficient subjects without a genetic diagnosis. PMID: 17303779
  67. phospholipid and cholesteryl ester transfer proteins are important in very low and high-density lipoprotein metabolism and display concerted actions in patients with type 2 diabetes [review] PMID: 17495597
  68. in non-diabetic, non-smoking subjects low rather than high PLTP activity is a marker for the presence of peripheral arterial disease PMID: 17553507
  69. the potential interaction between human plasma phospholipid transfer protein (and apolipoprotein A-I (apoA-I), double transgenic animals (hPLTPtg/hApoAItg) were compared with hApoAItg mice. PMID: 17761633
  70. PTLP activity is positively associated with serum ALT1, GOT1 and GOT2 in type 2 diabetes mellitus and metabolic syndrome. PMID: 17877759
  71. Cholesterol efflux from macrophage foam cells is enhanced by active PLTP through generation of two types of acceptor particles. PMID: 17900150
  72. Increased PLTP activity associated with the impaired cellular cholesterol efflux in type 2 diabetic subjects with coronary artery disease. PMID: 17917406
  73. it is concluded that apoE enhances the capacity of PLTP to remodel HDL but reduces the ability of HDL to participate in PLTP-mediated phospholipid transfers. PMID: 17921435
  74. ABCA1-dependent cholesterol efflux requires an amphipathic helical region of the N-terminal barrel of phospholipid transfer protein PMID: 18287097
  75. Acute elevation of PLTP activity destabilizes atherosclerotic lesions and aggravates pre-existing atherosclerosis. PMID: 18421000
  76. Association of rs2294213 with HDL-C levels into the extremes of the HDL distribution. PMID: 19013296
  77. Elevation of systemic PLTP, but not macrophage-PLTP, impairs macrophage reverse cholesterol transport in transgenic mice. PMID: 19100548
  78. The results confirm the atherogenic behavior of PLTP in coronary artery disease. PMID: 19135989
  79. These findings indicate that plasma HDL level is an important regulator of plasma PLTP and might play a role in the stabilization of PLTP in plasma. PMID: 19422933
  80. PLTP may be an important modulator of signal transduction pathways in human neurons. PMID: 19472218

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Subcellular Location Secreted
Protein Families BPI/LBP/Plunc superfamily, BPI/LBP family
Tissue Specificity Wide tissue distribution. Placenta > pancreas > lung > kidney > heart > liver > skeletal muscle > brain.
Database Links

HGNC: 9093

OMIM: 172425

KEGG: hsa:5360

STRING: 9606.ENSP00000361508

UniGene: Hs.439312

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