Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PLTP

Uniprot No.

P55058

Research Area

Transport

Alternative Names

BPI fold containing family E; BPIFE; HDLCQ9; High density lipoprotein cholesterol level quantitative trait locus 9; included; Lipid transfer protein II; OD107; Phospholipid transfer protein; Plasma phospholipid transfer protein ; Pltp; PLTP_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

18-493aa

Target Protein Sequence

EFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGHFYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFFYDGGYINASAEGVSIRTGLELSRDPAGRMKVSNVSCQASVSRMHAAFGGTFKKVYDFLSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

80.1kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human PLTP recombinant protein was produced in E.coli, where the gene sequence encoding Human PLTP (18-493aa) was expressed with the N-terminal GST tag. The purity of this PLTP protein was greater than 90% by SDS-PAGE.
One of the primary functions of PLTP is to facilitate the transfer of different categories of lipids, such as phospholipids and triglycerides, between various lipid carriers. Specifically, it promotes lipid transfer between high-density lipoprotein (HDL) and low-density lipoprotein (LDL), which is essential for maintaining lipid balance in the bloodstream. PLTP plays a crucial role in lipid metabolism. It helps clear excess cholesterol from the body and facilitates the return of cholesterol from peripheral tissues to the liver, contributing to cardiovascular health. Additionally, PLTP is involved in the repair and maintenance of cell membranes, which is essential for preserving cell integrity.
Aberrant activity or expression of PLTP is associated with some diseases. For instance, insufficient PLTP activity can lead to disruptions in cholesterol metabolism, increasing the risk of cardiovascular diseases. Furthermore, PLTP is linked to the pathogenesis of conditions like obesity, metabolic syndrome, and diabetes.

Target Background

Function

Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves. Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Plays an important role in HDL remodeling which involves modulating the size and composition of HDL. Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability.

Gene References into Functions

Study demonstrated that PLTP is overexpressed in synovial tissue of patients with chronic inflammatory rheumatisms, such as rheumatoid arthritis (RA), when compared to osteoarthritis patients (OA); and that RA but not OA patients displayed elevated levels of PLTP activity in synovial fluid, which were correlated with pro-inflammatory cytokine levels. PMID: 29565987
These results indicate that PLTP and MASP-1 can serve as plasma biomarkers for the early diagnosis and treatment of Age-related macular degeneration , which is critical for preventing Age-related macular degeneration -related blindness. PMID: 27605007
PLTP activity did not affect this metric. Thus, deviations in PLTP activity from the wild-type level reduce HDL mass and ex vivo cholesterol efflux capacity, but not the rate of macrophage cholesterol efflux to plasma or in vivo mRCT. PMID: 28137768
serum CETP and PLTP activity in patients diagnosed with hypothyroidism, was examined. PMID: 27899788
PLTP may regulate cigarette smoke extract-induced IL-8 expression via the ERK1/2 signaling pathway in human pulmonary epithelial cells PMID: 27596005
LPL and PLTP appear to be novel glioma-associated proteins and play a role in the progression of human glioma PMID: 27864281
association of PON-1 activity with PLTP activity was lost in analysis which included large HDL particles PMID: 26656640
a novel association between PLTPa and PON1 activity PMID: 26009633
The domain of apolipoprotein E4 required for PLTP activation resides within its amino-terminal 1-185 region. PMID: 26337529
Elevated baseline plasma levels of PLTP are associated with an increased risk of long-term all-cause mortality in patients with diabetes and known or suspected coronary disease. PMID: 25710294
This review summarizes the recent progresses made in the PLTP research field and focuses on the complexity of the implication of PLTP in obesity, insulin resistance and type 2 diabetes mellitus. [review] PMID: 25107452
Elevated plasma PLTP activity may predict an increased risk of T2DM in men PMID: 25304745
High systemic PLTP expression does not contribute significantly to a renal phenotype despite being implicated in systemic atherosclerosis in ApoE deficient mice. PMID: 25651711
proteolytic cleavage of PLTP by cathepsin G may enhance the injurious inflammatory responses that occur in COPD PMID: 24532668
PLTP is actively involved in lipid transfer, cholesterol efflux, HDL genesis, and remodeling at the blood-brain barrier. PMID: 24369175
Lower plasma CETP or higher PLTP activity was each associated with a significantly increased risk of cardiovascular disease. PMID: 23477743
Higher PLTP activity is associated with depressed LV systolic function in a dose-dependent manner. PMID: 23545183
In all the models with ApoE deficiency, elevated human PLTP expression causes higher levels of diet-induced atherosclerosis coinciding with decreased plasma levels of HDL cholesterol. PMID: 23313246
The physiological relevance of PLTP in intracellular lipid metabolism and signal transduction. [Review] PMID: 21736953
PLTP mediates PL transfer and participates in reverse cholesterol transport pathways at the fetoplacental barrier. PMID: 22492872
we provide evidences that induction of TGF-B1 production and Smad3 phosphorylation by Ox-LDL is mediated by Ras/ERK/PLTP pathway in human alveolar epithelial cells. PMID: 22034170
diabetes-environment and diabetes-gene interactions govern plasma PLTP activity PMID: 21973210
Pltp expression is increased in GDM with hyperglycemia and/or hyperinsulinemia contributing. High PLTP activity in fetal serum may enhance conversion of HDL into cholesterol-accepting particles, thereby increasing maternal-fetal cholesterol transfer. PMID: 22090281
PLTP activity could contribute to elevated cardiovascular risk in the presence of obesity and insulin resistance PMID: 21481395
PLTP has anti-inflammatory capabilities in macrophages. PMID: 21782857
Increased PLTP activity in human tear fluid in dry eye syndrome patients suggests an ocular surface protective role for this lipid transfer protein. PMID: 21514421
Data show that the removal of the carbohydrate chains had a quantitative impact on cellular secretion of PLTP and its phospholipid transfer activity. PMID: 21515415
data show the existence of small inactive plasma PLTP complexes, and show that variation in the amount of the two clusters of inactive PLTP complexes and specific activity of active PLTP contribute to the variation in plasma PLTP specific activity. PMID: 21303701
the SR-BI pathway contributes in unique ways to cholesterol metabolism and atherosclerosis susceptibility even in the presence of CETP PMID: 21454568
Human PLTP expression in transgenic rabbits worsens atherosclerosis as a result of increased levels of atherogenic apoB-containing lipoproteins. PMID: 21252068
CETP and PLTP activities were negatively related with the ratio of HDL-C/LDL-C. PMID: 20937151
a dual role for PLTP in human tear fluid PMID: 20724654
Our results indicate that there is no genetic association between PLTP and Alzheimer's disease PMID: 20714154
PLTP was overexpressed in the ER-negative breast cancer group. PMID: 20805453
Data show that biglycan (BGN) induces of phospholipid transfer protein (PLTP) in aortic valve interstitial cells via stimulation of Toll-like receptor 2, so increased BGN in stenotic valves contributes to the production of PLTP via TLR 2. PMID: 20382708
A gene score based on 2 PLTP single nucleotide polymorphisms is associated with lower PLTP transcription and activity, an increased number of HDL particles, smaller HDL size, and decreased risk of cardiovascular disease PMID: 20644014
apoA-I enhances the phospholipid transfer activity of PLTP secreted from macrophage foam cells without affecting the PLTP mass. PMID: 20534134
PLTP was found to mediate transfer of anionic phospholipids to HDL/LDL lipoproteins thereby neutralizing the effect of procoagulant liposomes, resulting in a reduction of procoagulant activity. PMID: 20088939
PLTP activity is strongly influenced by PLTP region polymorphisms and metabolic factors. PMID: 19965587
serum PLTP is involved in the clearance of postprandial lipoproteins and this process is attenuated in diabetes. PMID: 20108050
PLTP activity may represent a novel marker of left ventricular systolic dysfunction in patients with known or suspected coronary artery disease. PMID: 19446293
Human PLTP expressed in plasma from transgenic mice contributes to the formation of pre beta-HDL, whereas the expression of human cholesterol ester transfer protein does not affect this process. PMID: 11716766
This protein and pre-beta-high density lipoprotein are degraded by mast cell chymase, the high affinity efflux of cholesterol from macrophage foam cells is impaired. PMID: 12531890
The phospholipid transfer protein gene is a liver X receptor target expressed by macrophages in atherosclerotic lesions. PMID: 12612088
Elevation of PLTP activity results in rapid disposal of cholesterol from the body via increased conversion into bile acids and subsequent excretion. PMID: 12649089
PLTP is expressed by macrophages, is regulated by cholesterol loading, and is present in atherosclerotic lesions PMID: 12730304
elevated PLTP activity in hypertriglyceridemia is related to insulin resistance and not to increased PLTP mass PMID: 12754275
HepG2-derived PLTP resembles in several aspects the high-activity form of PLTP found in human plasma PMID: 12810820
PLTP is present in human atherosclerotic lesions, and its distribution suggests roles for PLTP in both cellular cholesterol metabolism and lipoprotein retention on extracellular matrix PMID: 12835223
seminal PLTP concentrations represented 25% of the concentration measured in blood plasma; seminal PLTP activity was partially associated with prostasomes; higher PLTP activity was measured in seminal plasma samples with low seminal vesicle secretions PMID: 12837922

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Subcellular Location

Secreted. Nucleus.

Protein Families

BPI/LBP/Plunc superfamily, BPI/LBP family

Tissue Specificity

Widely expressed. Highest level of expression in the ovary, thymus and placenta, with moderate levels found in the pancreas, small intestine, testis, lung and prostrate. Low level expression in the kidney, liver and spleen, with very low levels found in t

Database Links

HGNC: 9093

OMIM: 172425

KEGG: hsa:5360

STRING: 9606.ENSP00000361508

UniGene: Hs.439312

Code	CSB-EP018212HU
Abbreviation	Recombinant Human PLTP protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Phospholipid transfer protein (PLTP)

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