Recombinant Human Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP)

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Code CSB-EP867114HU
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Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
LHPP
Uniprot No.
Research Area
Metabolism
Alternative Names
FLJ44846; FLJ46044; HDHD2B; hLHPP; lhpp; LHPP_HUMAN; Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-270aa
Target Protein Sequence
MAPWGKRLAGVRGVLLDISGVLYDSGAGGGTAIAGSVEAVARLKRSRLKVRFCTNESQKSRAELVGQLQRLGFDISEQEVTAPAPAACQILKEQGLRPYLLIHDGVRSEFDQIDTSNPNCVVIADAGESFSYQNMNNAFQVLMELEKPVLISLGKGRYYKETSGLMLDVGPYMKALEYACGIKAEVVGKPSPEFFKSALQAIGVEAHQAVMIGDDIVGDVGGAQRCGMRALQVRTGKFRPSDEHHPEVKADGYVDNLAEAVDLLLQHADK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
45.2kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Discover our Recombinant Human LHPP, a valuable research tool for investigators studying the role of Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (hLHPP) in metabolism. This protein plays an essential role in the regulation of phosphate metabolism, contributing to crucial cellular processes and metabolic pathways.

Our Recombinant Human LHPP offers the full length protein (1-270aa) to ensure accurate representation for your research objectives. Expressed in E. coli, this protein features an N-terminal 6xHis-SUMO tag for easy purification and detection. With a purity of greater than 90% as determined by SDS-PAGE and available in both liquid and lyophilized powder forms, this high-quality recombinant protein provides the reliability and performance required for your metabolism-centered research.

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Target Background

Function
Phosphatase that hydrolyzes imidodiphosphate, 3-phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency.
Gene References into Functions
  1. LHPP suppresses cell proliferation and metastasis in cervical cancer, and promotes apoptosis by suppressing AKT activation. PMID: 29944886
  2. in patients with hepatocellular carcinoma, low expression of LHPP correlated with increased tumour severity and reduced overall survival; thus LHPP is a protein histidine phosphatase and tumour suppressor, suggesting that deregulated histidine phosphorylation is oncogenic PMID: 29562234
  3. LHPP is associated with the interplay of risky sexual behaviors and alcohol dependence. PMID: 27531626
  4. Patients with major depressive disorder with LHPP rs35936514 CT/TT genotype have increased activity in certain brain regions. PMID: 27843651
  5. LHPP gene variation is associated with oral cavity and pharyngeal cancer. PMID: 27749845
  6. Lhpp or a product of a collinear brain-specific transcript, therefore, may interact with Htr1a in the pathogenesis of major depression PMID: 18268499

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
HAD-like hydrolase superfamily
Tissue Specificity
Expressed in brain, and at lower levels in liver and kidney. Detected in thyroid (at protein level). Expressed in liver, kidney and moderately in brain.
Database Links

HGNC: 30042

OMIM: 617231

KEGG: hsa:64077

STRING: 9606.ENSP00000357835

UniGene: Hs.527748

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