Recombinant Human Plasma membrane calcium-transporting ATPase 4(ATP2B4) ,partial

Code CSB-YP002338HU
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Source Yeast
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Code CSB-EP002338HU
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Source E.coli
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Code CSB-EP002338HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002338HU
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Source Baculovirus
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Code CSB-MP002338HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ATP2B4
Uniprot No. P23634
Alternative Names AT2B4_HUMAN; ATP2B2; Atp2b4; ATPase Ca++ transporting plasma membrane 4; Cation transporting ATPase; DKFZp686G08106; DKFZp686M088; Matrix remodelling associated 1; Matrix remodelling associated protein 1; Matrix-remodeling-associated protein 1; MXRA 1; MXRA1; OTTHUMP00000034088; OTTHUMP00000034089; Plasma membrane calcium ATPase 4; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4; Plasma membrane calcium transporting ATPase 4; Plasma membrane calcium-transporting ATPase 4; PMCA 4; PMCA4; PMCA4b; PMCA4x; Sarcolemmal calcium pump
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell
Gene References into Functions
  1. Data (including data from studies in knockout mice) suggest that renalase functions as a protective plasma protein that reduces pancreatic acinar cell injury and prevents pancreatitis via interactions with plasma membrane calcium ATPase PMCA4b. PMID: 29042438
  2. Na+, K+-ATPase and Ca2+-ATPase activity and Na+, K+-ATPase alpha4 and PMCA4 isoform expressions examined in asthenozoospermic men PMID: 28787189
  3. study provides evidence for the therapeutic potential of targeting PMCA4 to improve VEGF-based pro-angiogenic interventions. This goal will require the development of refined, highly selective versions of ATA, or the identification of novel PMCA4 inhibitors. PMID: 28684310
  4. data suggest that an altered regulation of gene expression is responsible for the reduced RBC-PMCA4b levels that is probably linked to the development of human disease-related phenotypes. PMID: 28216081
  5. the ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility PMID: 28714864
  6. Increased plasma membrane abundance of PMCA4b in vemurafenib-treated BRAF mutant cells is associated with enhanced Ca2+ clearance. PMID: 27813079
  7. The role of heterozygous variants of ATP2B4 is regulated by HSPG2 protein with transcription factors in bone formation through modulation of calcium signaling. PMID: 28327142
  8. RNA sequencing identified a novel ATPase, Ca2+ transporting, plasma membrane 4(ATP2B4)-protein kinase C-alpha (PRKCA) fusion transcript. PMID: 26676968
  9. our data show that CD147 interacts via its immunomodulatory domains with PMCA4 to bypass TCR proximal signaling and inhibit IL-2 expression. PMID: 26729804
  10. We also nominate a new candidate gene in congenital arrhythmia, ATP2B4, and provide experimental evidence of a regulatory role for variants discovered using this framework. PMID: 26448358
  11. p.R268Q mutation in PMCA4 resulted in functional changes in calcium homeostasis in human neuronal cells. This suggests that calcium dysregulation may be associated with the pathogenesis of Familial spastic paraplegia PMID: 25798335
  12. The slowly activating PMCA4b isoform produced long-lasting Ca2+ oscillations in response to store-operated Ca2+ entry. PMID: 25690014
  13. Mechanism of PMCA4 that creates lipid asymmetry is well understood in terms of ATP hydrolysis; molecular models exist for trajectory taken by phospholipid substrates through the enzyme. [review-like article] PMID: 25233416
  14. Calcium dysregulation resulting from a novel missense mutation (c.803G>A, p.R268Q) in the PMCA4 (ATP2B4) gene may be associated with the pathogenesis of familial spastic paraplegia. PMID: 25119969
  15. PMCA4 inhibits the activation of the calcineurin/NFAT pathway on VEGF stimulation of endothelial cells, leading to a significant attenuation of VEGF-mediated angiogenesis. PMID: 25147342
  16. No significant relationship was detected in the level of expression of the ATP2B4 and ATP5B genes in cancerous and healthy tissues of colorectal cancer patients. PMID: 24583174
  17. A significant relationship between ATP2B4 gene expression and the tumor location was detected in patients with rectum tumors. PMID: 24583174
  18. a di-leucine-like internalization signal at the C-tail of PMCA4b, is reported. PMID: 23830917
  19. The PMCA4b enriched in lipid rafts decreases the local [Ca2+] levels, downregulating the nNOS activity dynamically associated to PMCA4b via Ca2+-dependent PDZ domain-mediated protein- protein interactions. PMID: 23549614
  20. An ATP2B4 variant reduces the odds of P. falciparum infection in pregnancy and mitigates the odds of associated maternal anemia. PMID: 23444010
  21. High PMCA4 gene expression correlates with high peak bone mass in humans. PMID: 23266958
  22. GUSB and ATP2B4 have been validated as a reliable gene combination for Cystic Fibrosis Transmembrane Conductance Regulator gene qPCR data normalization. PMID: 22525089
  23. Alternative pathways for association and dissociation of the calmodulin-binding domain of plasma membrane Ca(2+)-ATPase isoform 4b (PMCA4b). PMID: 22767601
  24. Genome-wide association study for severe malaria identified a locus on chromosome 1q32 within the ATP2B4 gene, and another locus on chromosome 16q22.2, possibly linked to a neighbouring gene encoding the tight-junction protein MARVELD3 PMID: 22895189
  25. The results of this study suggest posttranscriptional regulation of PMCA4 during carcinogenesis. PMID: 22480210
  26. The recently emerging role of the plasma membrane calcium/calmodulin dependent ATPase isoform 4 (PMCA4) in regulating calcium signalling, is reviewed. PMID: 21167220
  27. PMCA4 is strongly expressed in rabbit CE and its immunolocalization exhibits marked changes in distribution during the wound healing process. PMID: 21139678
  28. regulatory domain works independently of its terminal localization and if auto-inhibitory domains PMID: 21073872
  29. Data show that the anchors correspond to Phe-1110 and Trp-1093, respectively, in full-length PMCA4b, and the peptide and CaM are oriented in an anti-parallel manner. PMID: 19996092
  30. We demonstrate here that, in the early phase of apoptosis, hPMCA4b is cleaved at aspartic acid Asp(1080) in hPMCA4b-transfected COS-7 cells or in HeLa cells that naturally express this protein PMID: 11751908
  31. plasma membrane Ca2+ pump 4b/CI binds to Ca2+/calmodulin-dependent membrane-associated kinase CASK PMID: 12511555
  32. co-immunoprecipitation, treatment with tyrosine kinase inhibitors and integrin inhibition experiments suggest that FAK is responsible for PMCA4b tyrosine phosphorylation during platelet activation. PMID: 12540962
  33. results suggest that a decrease in PMCA4b expression in Meg-01 cells is compensated to maintain normal intracellular calcium levels. PMID: 12944246
  34. functional association with RASSF1 indicates a role for PMCA4b in the modulation of Ras-mediated signaling PMID: 15145946
  35. residue Asp(170), in the putative "A" domain of human PMCA isoform 4xb, plays a critical role in autoinhibition PMID: 15292209
  36. a novel functional interaction between PMCA and calcineurin, suggesting a role for PMCA as a negative modulator of calcineurin-mediated signaling pathways in mammalian cells. PMID: 15955804
  37. The characteristics of the fragment of hPMCA4b produced by caspase-3 are reported. PMID: 16080782
  38. Although differences in PMCA4 mRNA levels were observed between breast cell lines, they were not of the magnitude observed for PMCA2. PMID: 16216224
  39. PMCA4 is a good housekeeping gene for normalization of gene expression for polytopic membrane proteins including transporters and receptors. PMID: 16978418
  40. physiological relevance of the interaction between PMCA4b and nNOS and suggests its signaling role in the heart PMID: 17242280
  41. PMCA4b is localized in non-filamentous actin complexes in resting platelets by means of PDZ domain interactions and then associates with the actin cytoskeleton during cytoskeletal rearrangement upon platelet activation. PMID: 17393022
  42. Compared with the expression of PMCA4b upon platelet maturation, platelets from diabetic patients exhibit similarities with immature megakaryocytes PMID: 17883705
  43. platelets in hypertensive patients demonstrated a major increase in plasma membrane Ca(2+)ATPase 4b (PMCA4b) expression when compared with normal controls PMID: 17957572
  44. PMCA4 seems to be preferentially distributed in both human syncytiotrophoblast plasma membranes. PMID: 18657858
  45. Results suggest that PSD-95 promotes the formation of high-density PMCA4b microdomains in the plasma membrane and that the membrane cytoskeleton plays an important role in the regulation of this process. PMID: 19073225
  46. Pmca4b likely reduces the local Ca2+ signals involved in reactive cardiomyocyte hypertrophy via calcineurin regulation. PMID: 19287093
  47. PMCA4 is significantly (P < 0.000001) downregulated early in the progression of some colon cancers as these cells become less differentiated PMID: 19755660
  48. This protein has been found differentially expressed in patients with schizophrenia. PMID: 19034380

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Subcellular Location Cell membrane, Multi-pass membrane protein, Cell projection, cilium, flagellum membrane, Multi-pass membrane protein
Protein Families Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIB subfamily
Tissue Specificity Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.
Database Links

HGNC: 817

OMIM: 108732

KEGG: hsa:493

STRING: 9606.ENSP00000350310

UniGene: Hs.343522

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