Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PABPC1

Uniprot No.

P11940

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

PABPC1; PAB1; PABP1; PABPC2; Polyadenylate-binding protein 1; PABP-1; Poly(A)-binding protein 1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-370aa

Target Protein Sequence

MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

57.8kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The synthesis of this Recombinant Human PABPC1 protein depends on the utilization of recombinant DNA technology. DNA sequences that encoded the PABPC1 protein could be inserted into a vector and introduced into an expression host, E.coli, where it could be easily expressed in and purified from. The expression of this PABPC1 protein was at 1-370aa. N-terminal 6xHis-SUMO tag was fused with this protein. The purity is 90%+ determined by SDS-PAGE.

PABPC1 (also known as PAB1 or PABP) is gene encoding a protein named polyadenylate-binding protein 1 (PABPC1 or PABP-1) in human. The protein encoded by this gene is also known as poly(A)-binding protein 1. Increasing studies has shown that the protein encoded by PABPC1 gene is cleaved during poliovirus and coxsackievirus infection by viral 3Cprotease and that 3Cprotease modification of a subset of PABP can result in significant translation inhibition. The encoded protein can bind to mRNA 3'-UTR, poly(A), poly(U) RNA and protein C-terminus. It is involved in multiple biological processes with its translation activator activity.

Target Background

Function

Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability.; (Microbial infection) Positively regulates the replication of dengue virus (DENV).

Gene References into Functions

Study characterized the structure of multiple PABPC1 molecules bound to a long poly(A) chain as constituting a one-dimensional wormlike form. These interactions responsible for PABPC1 multimerization occur at residues in the linker region and the RNA recognition motif 2, and shown that such interaction is important for translational activity. PMID: 29362417
Epstein-Barr Virus protein EB2 first is recruited to the mRNA cap structure in the nucleus and then interacts with the proteins eIF4G and PABP to enhance the initiation step of translation. PMID: 29142127
These findings identify PABPC1 as a direct regulator of cardiac hypertrophy and define a new paradigm of gene regulation in the heart, where controlled changes in poly(A) tail length influence mRNA translation. PMID: 28653618
Data suggest that DEAD-box helicase 3 (DDX3X) physically interacts and co-localizes with poly(A)-binding cytoplasmic protein 1 (PABPC1) and caprin-1 in lamellipodia at the leading edge of spreading cells; these interactions are dependent on mRNA; depletion of DDX3X (via gene silencing with the CRISPR-Cas system) leads to decreased cell motility. These studies were conducted using MRC5 lung fibroblast cell line. PMID: 28733330
Data suggest that hnRNPLL specifically associates with cytoplasmic PABPC1 in both T-lymphocytes and plasma cells; PABPC1 promotes binding of hnRNPLL to immunoglobulin H (IgH, heavy chain) mRNA and regulates switching from mIgH (membrane isoform) to sIgH (secreted isoform) in plasma cells. (hnRNPLL = heterogeneous nuclear ribonucleoprotein L-like protein; PABPC1 = cytoplasmic poly[A]-binding protein 1) PMID: 28611064
PABP enhances the productive binding of the eRF1-eRF3 complex to the ribosome, via interactions with the N-terminal domain of eRF3a which itself has an active role in translation termination. PMID: 27418677
Unr stimulated the binding of PABP1 to mRNA, and that Unr was required for the stable interaction of PABP1 and eIF4G in cells. PMID: 26936655
PABPC1 decreased expression in in infertile men with non-obstructive azoospermia PMID: 26843391
BTG2 stimulates mRNA deadenylation via CAF1 activation through interaction with PABPC1. Interaction of BTG2 with the first RRM domain of PABPC1 is required for BTG2 to control cell proliferation. PMID: 26912148
PABPC1 is a novel co-regulator of the AR PMID: 26176602
PABPC1 is upregulated in gastric carcinoma tissues, and high PABPC1 expression predicts poor survival. PABPC1 regulates proliferation and transformation of gastric cancer cells in vitro and in vivo. PABPC1 knockdown induces apoptosis. PMID: 26097561
PABPC1 interacts with AGO2 and is responsible for the microRNA mediated gene silencing in high grade hepatocellular carcinoma. PMID: 26188282
Poly(A) binding protein 1 enhances cap-independent translation initiation of neurovirulence factor from avian herpesvirus PMID: 25503397
Metastatic duodenal cancer cells do not tolerate intracellular PABP1 accumulation and are thus exported into the extracellular milieu by the exosome-mediated pathway. In addition, PABP1 has a potential use as a biomarker for metastatic duodenal cancer. PMID: 25065644
This study highlights PABP-1 as a protein that is important to the pathology of ALS and indicates that the proteomic profile of TDP-43 inclusions in ALS may differ depending on the causative genetic mutation. PMID: 25111021
PABPC1 is mislocalized in spinal cord motor neurons in amyotrophic lateral sclerosis. PMID: 24336168
Study shows that PABP specifically regulates iNOS mRNA stability in human DLD-1 cells by binding to different sites in the 50- and 30-UTR of the iNOS mRNA. . PMID: 23711718
These results suggest that production of the NSs protein during rift valley fever virus infection leads to sequestration of PABP1 in the nuclear speckles, creating a state within the cell that favors viral protein production. PMID: 23966414
These data indicate that PABPC1 is an important cellular target of human herpesvirus 8 ORF57 to directly upregulate polyadenylated nuclear RNA accumulation during viral lytic infection. PMID: 23077296
Data show that eIF4G interacts with the RRM2 domain of polyadenylate-binding protein-1 (PABP). PMID: 23041282
data suggest that PABPC1 is essential for the formation of L1 RNA-protein complexes and may play a role in L1 RNP translocation in the host cell PMID: 22907758
EMCV 3C proteinase mediates site-specific PABP cleavage and demonstrate that PABP cleavage by 3C regulates EMCV replication PMID: 22837200
This finding demonstrates that viruses can increase host translation initiation factor concentration to foster their replication and defines a unique mechanism whereby control of PABP abundance regulates eIF4F assembly. PMID: 22431630
a role for PABPC1 and associated translation initiation factors in nonsense-mediated mRNA decay (NMD) evasion of AUG-proximal nonsense-mutated transcripts PMID: 21989405
study presents evidence that the function of KSHV polyadenylated nuclear (PAN) RNA is to bind poly(A) binding protein, which normally binds poly(A) tails of mRNAs in the cytoplasm but is re-localized into the nucleus during lytic KSHV infection PMID: 22022268
A direct link between PABP1 modification status and the formation of distinct mRNP (messenger ribonucleoprotein) complexes that regulate mRNA fate in the cytoplasm. PMID: 22004688
The present study has characterized DDX3 as a pivotal SG-nucleating factor and illustrates co-ordinative roles for DDX3, eIF4E and PABP1 in integrating environmental stress with translational regulation. PMID: 21883093
Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to UV irradiation reveals mRNA-dependent export PMID: 21940797
in the absence of PABP, the glycolytic enzyme GAPDH translocated to the cell nucleus and activated the GAPDH mediated apoptotic pathway by enhancing acetylation and serine 46 phosphorylation of p53. PMID: 21539808
these results suggest that depletion of PABP prevents protein synthesis and consequently leads to cell death through apoptosis. PMID: 21521633
LARP4 activity is integrated with other PAM2 protein activities by PABP as part of mRNA homeostasis. PMID: 21098120
Cytoplasmic PABP accumulation is translationally controlled in human cytomegalovirus-infected cells. PMID: 20980505
These findings reveal that despite species-specific differences in the relative strength of the PABPC1-binding sites, the interaction between GW182 proteins and PABPC1 is critical for miRNA-mediated silencing in animal cells. PMID: 21063388
Data show that in addition to PP2Ac, alpha4 interacts with EDD and PABP, suggesting its involvement in multiple steps in the mTOR pathway that leads to translation initiation and cell-cycle progression. PMID: 20544796
The cytoplasmic poly (A)-binding protein (PABP) plays a crucial role in regulating both translation and stability of eukaryotic mRNA. PMID: 20009508
PABP relocalization in infection was found to be independent of the viral protein ICP27. PMID: 20573819
Structural basis of binding of P-body-associated proteins GW182 and ataxin-2 by the Mlle domain of poly(A)-binding protein.( PMID: 20181956
HIV- 1 protease inhibits Cap- and poly(A)-dependent translation upon eIF4GI and PABP cleavage PMID: 19956697
The side chain of the invariant MLLE residue K580 of poly(A)-binding protein forms hydrogen bonds with the backbone of PAM2 residues 5 and 7. PMID: 20096703
The authors show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. PMID: 20098421
Bunyamwera virus triggers nuclear retention of PABP in mammalian cells. PMID: 19193790
Poly(A)-binding protein modulates mRNA susceptibility to cap-dependent miRNA-mediated repression. PMID: 19934229
Methylated in vivo by CARM1. Methylated region is mapped. PMID: 11850402
PABP1 is a potential substrate in MAPKAP kinase 2-induced mRNA stabilization. PMID: 12565831
targeted degradation of PABP contributes to translation inhibition in apoptotic cells PMID: 14739600
PABP1 associates with paxillin in order to be efficiently transported from the nucleus to the cytoplasm; this event is necessary for cells to remodel their focal adhesions during cell migration. PMID: 15831480
a novel signaling pathway involving MKK-2 and ERK1/2 may down-regulate the activity of PABP and eIF4E by controlling their phosphorylation and compensates for the effect of excess cellular PABP PMID: 16332685
In esophageal cancer, reduced expression of PABPC1 was correlated with local tumor progression and poor prognosis after surgery. PMID: 16465428
PABP interacts with HHV-8 K10/10.1 protein in infected primary effusion lymphoma (PEL) cell lines. PMID: 16716377
poly(A)-binding protein 1 (PABP) as a novel BRCA1-interacting protein. PMID: 16782705

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Subcellular Location

Cytoplasm. Cytoplasm, Stress granule. Nucleus. Cell projection, lamellipodium.

Protein Families

Polyadenylate-binding protein type-1 family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 8554

OMIM: 604679

KEGG: hsa:26986

STRING: 9606.ENSP00000313007

UniGene: Hs.387804

Code	CSB-EP017352HU
Abbreviation	Recombinant Human PABPC1 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Polyadenylate-binding protein 1 (PABPC1), partial

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