Recombinant Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1), partial

Code CSB-YP010216HU
MSDS
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Source Yeast
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Code CSB-EP010216HU
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Source E.coli
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Code CSB-EP010216HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010216HU
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Source Baculovirus
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Code CSB-MP010216HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
BCNG-1; BCNG1; Brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC 2; HAC2; HCN1; HCN1_HUMAN; Hyperpolarization activated cyclic nucleotide gated potassium channel 1; Potassium channel; voltage-gated; brain; 1; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli.
Gene References into Functions
  1. Polymorphism of HCN1 is associated with breast cancer. PMID: 28178648
  2. Study presents cryo-electron microscopy structures of the human HCN1 channel in the absence and presence of cAMP at 3.5 A resolution. HCN channels contain a K(+) channel selectivity filter-forming sequence from which the amino acids create a unique structure that explains Na(+) and K(+) permeability. PMID: 28086084
  3. A new mode of regulating HCN1 trafficking: through the use of a di-arginine ER retention signal that monitors processing of the channel in the early secretory pathway. PMID: 25142030
  4. de novo HCN1 point mutations cause a recognizable early-onset epileptic encephalopathy in humans PMID: 24747641
  5. acute abrogation of HCN1-FLNa interaction in neurons, with the use of decoy peptides that mimic the FLNa-binding domain of HCN1, abolishes the punctate distribution of HCN1 channels in neuronal cell bodies PMID: 24403084
  6. Studies suggest that HCN1 channels may be therapeutic targets for treatment of depressive disorders. PMID: 23033536
  7. Wild-type presynaptic HCN1 channel function is persistently decreased following seizures. PMID: 23077068
  8. HCN1 channels make an important contribution to the maintenance of spontaneous burst activity in embryonic cortical neuron cultures. PMID: 22094222
  9. Hyperpolarization-activated currents are smaller and slower, input resistances are higher, and membrane time constants are longer in HCN1-deficient than in HCN1-expressing neurons of the ventral cochlear nucleus. PMID: 21562186
  10. Genetic analysis in 48 Sudden unexpected death in epilepsy cases identified six novel and three previously reported nonsynonymous (amino acid changing) variants in HCN1 , HCN2, HCN3 and HCN4. PMID: 21615589
  11. increasing cAMP levels in cells antagonized the up-regulation of HCN1 channels mediated by a TRIP8b construct binding the CNBD exclusively. PMID: 21504900
  12. Human HCN1 hyperpolarization activated current (Ih) amplitude is rapidly enhanced after establishment of the whole-cell configuration in HEK293 cells. PMID: 20806410
  13. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20145138
  14. Helical secondary structure of the external S3-S4 linker of this pacemaker channel is examined and affects its activation PMID: 12668666
  15. HCN1 and HCN2 expression were measured using in situ hybridization and immunocytochemistry in hippocampi; the expression of HCN isoforms is dynamically regulated in human as well as in experimental hippocampal epilepsy PMID: 12890777
  16. In HCN1, the amino acid substitution A881T was identified in one idiopathic generalized epilepsy patient. PMID: 17931874
  17. The polymorphism rs10941679 near HCN1/MRPS30 was also associated with percent dense area in breast cancer. PMID: 19232126
  18. Observational study of gene-disease association. (HuGE Navigator) PMID: 19232126
  19. Observational study of gene-disease association. (HuGE Navigator) PMID: 18454440
  20. Observational study of gene-disease association. (HuGE Navigator) PMID: 17931874
  21. Observational study of gene-disease association. (HuGE Navigator) PMID: 18081024

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Involvement in disease
Epileptic encephalopathy, early infantile, 24 (EIEE24)
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Potassium channel HCN family
Tissue Specificity
Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
Database Links

HGNC: 4845

OMIM: 602780

KEGG: hsa:348980

STRING: 9606.ENSP00000307342

UniGene: Hs.353176

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