HCN1 Antibody

Datasheet

Code	CSB-PA010216LA01HU
Size	US$166
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Image	IHC image of CSB-PA010216LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA010216LA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA010216LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HCN1 Polyclonal antibody

Uniprot No.

O60741

Target Names

HCN1

Alternative Names

BCNG-1 antibody; BCNG1 antibody; Brain cyclic nucleotide gated channel 1 antibody; Brain cyclic nucleotide-gated channel 1 antibody; HAC 2 antibody; HAC2 antibody; HCN1 antibody; HCN1_HUMAN antibody; Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody; Potassium channel; voltage-gated; brain; 1 antibody; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 protein (784-887AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HCN1 Antibody (Product code: CSB-PA010216LA01HU) is Non-conjugated. For HCN1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA010216LB01HU	HCN1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA010216LC01HU	HCN1 Antibody, FITC conjugated
Biotin	CSB-PA010216LD01HU	HCN1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli.

Gene References into Functions

Polymorphism of HCN1 is associated with breast cancer. PMID: 28178648
Study presents cryo-electron microscopy structures of the human HCN1 channel in the absence and presence of cAMP at 3.5 A resolution. HCN channels contain a K(+) channel selectivity filter-forming sequence from which the amino acids create a unique structure that explains Na(+) and K(+) permeability. PMID: 28086084
A new mode of regulating HCN1 trafficking: through the use of a di-arginine ER retention signal that monitors processing of the channel in the early secretory pathway. PMID: 25142030
de novo HCN1 point mutations cause a recognizable early-onset epileptic encephalopathy in humans PMID: 24747641
acute abrogation of HCN1-FLNa interaction in neurons, with the use of decoy peptides that mimic the FLNa-binding domain of HCN1, abolishes the punctate distribution of HCN1 channels in neuronal cell bodies PMID: 24403084
Studies suggest that HCN1 channels may be therapeutic targets for treatment of depressive disorders. PMID: 23033536
Wild-type presynaptic HCN1 channel function is persistently decreased following seizures. PMID: 23077068
HCN1 channels make an important contribution to the maintenance of spontaneous burst activity in embryonic cortical neuron cultures. PMID: 22094222
Hyperpolarization-activated currents are smaller and slower, input resistances are higher, and membrane time constants are longer in HCN1-deficient than in HCN1-expressing neurons of the ventral cochlear nucleus. PMID: 21562186
Genetic analysis in 48 Sudden unexpected death in epilepsy cases identified six novel and three previously reported nonsynonymous (amino acid changing) variants in HCN1 , HCN2, HCN3 and HCN4. PMID: 21615589
increasing cAMP levels in cells antagonized the up-regulation of HCN1 channels mediated by a TRIP8b construct binding the CNBD exclusively. PMID: 21504900
Human HCN1 hyperpolarization activated current (Ih) amplitude is rapidly enhanced after establishment of the whole-cell configuration in HEK293 cells. PMID: 20806410
Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20145138
Helical secondary structure of the external S3-S4 linker of this pacemaker channel is examined and affects its activation PMID: 12668666
HCN1 and HCN2 expression were measured using in situ hybridization and immunocytochemistry in hippocampi; the expression of HCN isoforms is dynamically regulated in human as well as in experimental hippocampal epilepsy PMID: 12890777
In HCN1, the amino acid substitution A881T was identified in one idiopathic generalized epilepsy patient. PMID: 17931874
The polymorphism rs10941679 near HCN1/MRPS30 was also associated with percent dense area in breast cancer. PMID: 19232126
Observational study of gene-disease association. (HuGE Navigator) PMID: 19232126
Observational study of gene-disease association. (HuGE Navigator) PMID: 18454440
Observational study of gene-disease association. (HuGE Navigator) PMID: 17931874
Observational study of gene-disease association. (HuGE Navigator) PMID: 18081024

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Involvement in disease

Epileptic encephalopathy, early infantile, 24 (EIEE24)

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Potassium channel HCN family

Tissue Specificity

Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.

Database Links

HGNC: 4845

OMIM: 602780

KEGG: hsa:348980

STRING: 9606.ENSP00000307342

UniGene: Hs.353176

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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