Recombinant Human Prefoldin subunit 3 (VBP1)

Code CSB-YP025808HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP025808HU
MSDS
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Source E.coli
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Code CSB-EP025808HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025808HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP025808HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VBP1
Uniprot No.
Alternative Names
HIBBJ 46; HIBBJ46; PFD 3; PFD3; PFD3_HUMAN; PFDN 3; PFDN3; Prefoldin 3; Prefoldin subunit 3; Prefoldin3; VBP 1; VBP-1; VBP1; VHL binding protein 1; VHL-binding protein 1; Von Hippel Lindau binding protein 1; Von Hippel-Lindau-binding protein 1
Species
Homo sapiens (Human)
Expression Region
2-197
Target Protein Sequence
AAVKDSCGK GEMATGNGRR LHLGIPEAVF VEDVDSFMKQ PGNETADTVL KKLDEQYQKY KFMELNLAQK KRRLKGQIPE IKQTLEILKY MQKKKESTNS METRFLLADN LYCKASVPPT DKMCLWLGAN VMLEYDIDEA QALLEKNLST ATKNLDSLEE DLDFLRDQFT TTEVNMARVY NWDVKRRNKD DSTKNKA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Gene References into Functions
  1. Data show that von Hippel-Lindau-binding protein 1 (VBP1) enhances the stability of von Hippel-Lindau tumor suppressor protein (pVHL) and facilitates pVHL-mediated ubiquitination of hypoxia-inducible factor 1, alpha subunit (HIF-1alpha). PMID: 29121446
  2. VBP1 acts together with p97 to regulate hMSH4 degradation. PMID: 23964080
  3. binds to HIF-1 alpha and regulates the function of HIF-1 alpha PMID: 12613020
  4. VBP1 and cullin2/von Hippel-Lindau are required for proper HIV-1 expression at a step between integrase-dependent proviral integration into the host genome and transcription of viral genes PMID: 17698809
Subcellular Location
Cytoplasm. Nucleus. Note=In complex with VHL can translocate to the nucleus.
Protein Families
Prefoldin subunit alpha family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 12662

OMIM: 300133

KEGG: hsa:7411

STRING: 9606.ENSP00000286428

UniGene: Hs.436803

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