Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

DDX53

Uniprot No.

Q86TM3

Research Area

Cancer

Alternative Names

CAGE; Cancer associated gene protein; Cancer-associated gene protein; Cancer/testis antigen 26; CT26; DDX53; DDX53_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 53; DEAD box protein 53; DEAD box protein CAGE; Probable ATP dependent RNA helicase DDX53; Probable ATP-dependent RNA helicase DDX53

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-631aa

Target Protein Sequence

MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSRKPGQRRKEFYFLS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

76.2 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human DDX53 protein is a fusion protein consists of the human DDX53 protein (1-631aa) partnered with the N-terminal 10xHis tag and C-terminal Myc tag. It was produced in the E.coli. This recombinant DDX53 protein's purity is greater than 85% determined by SDS-PAGE. After electrophoresis, there is a 78 kDa protein band presented on the gel.

Human Probable ATP-dependent RNA helicase DDX53 functions as an oncogene and increases the level of cyclins. Aberrant expression of DDX53 has been documented in various cancer tissues and cell lines. Helicases are implicated in most stages of the gene expression pathway, ranging from DNA replication, RNA transcription, splicing, RNA transport, ribosome biogenesis, mRNA translation, RNA storage and decay. Besides, methylation has an important role for the expression regulation of DDX53. It has been found that DDX53 confers resistance to various anti-cancer drugs. Furthermore, a study noted that DDX53 promotes cancer stem cell-like properties and autophagy. DDX53 showed a co-expression with CD133, and binding to SOX-2, a marker of cancer stemness. Threrfore, DDX53 has been regared as a novel role of DDX53 in regulating cancer stem cell-like properties. Additionally, Activation of DDX53 was observed in a variety of tumors, including gastric and endometrial cancers, as well as in hematological cancers.

Target Background

Gene References into Functions

A novel role of DDX53 in regulating cancer stem cell-like properties by binding to SOX-2.DDX53 directly regulates the SOX-2 expression in drug-resistant melanoma cells. PMID: 28535666
DDX53 promotes stem cell-like properties, autophagy, and confers resistance to anti-cancer drugs in breast cancer cells. PMID: 28152297
Results provide evidence that miR-30a forms a positive feedback loop with CAGE to regulate the expression of p53 confering resistance to anti-cancer drugs. PMID: 26912082
miR-217-CAGE feedback loop serves as a target for overcoming resistance to various anti-cancer drugs, including EGFR and HER2 inhibitors. PMID: 26863629
Results show the direct regulation of CAGE expression by HDAC3 and that HDAC3-CAGE axis regulates the activation of EGFR. HDAC3 targets CAGE to regulate the tumorigenic potential and angiogenic potential of cancer cells. PMID: 26883907
CAGE induced the interaction between histone deacetylase 2 (HDAC2) and Snail, which exerted a negative effect on p53 expression in neoplastic cells; CAGE confers drug resistance by regulating expression of p53 through HDAC2. PMID: 20534591
CAGE is expressed in a variety of cancers but not in normal tissues except testis, and might play a role in cellular proliferation. PMID: 11922625
results suggest that the methylation status of the CpG sites of CAGE determines its expression, and that hypomethylation of CAGE precedes the development of gastric cancer and hepatocellular carcinoma PMID: 12849980
CAGE promotes motility of cancer cells through activation of focal adhesion kinase (FAK). PMID: 17028776
Hypomethylated CAGE promoter CpG islands are frequently involved in uterine cervical carcinogenesis. PMID: 17341616
The CAGE gene is widely expressed in various cancer tissues and cancer cell lines. PMID: 18388483

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Subcellular Location

Nucleus.

Protein Families

DEAD box helicase family

Tissue Specificity

Expressed in testis. Wide expression in various cancer tissues and cancer cell lines.

Database Links

HGNC: 20083

KEGG: hsa:168400

STRING: 9606.ENSP00000368667

UniGene: Hs.434416

Code	CSB-EP773024HU
Abbreviation	Recombinant Human DDX53 protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP773024HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DDX53. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP773024HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DDX53.
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Recombinant Human Probable ATP-dependent RNA helicase DDX53 (DDX53)

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